Background
Methods
Patients
NIND | CIS | MS | LNB | OIND | |
---|---|---|---|---|---|
Demographic data
| |||||
Samples | 20 | 30 | 20 | 8 | 30 |
Samples for Immuno-phenotyping CSF/Blood | 19/13 | 30/28 | 20/15 | 8/3 | 30/24 |
Age (years) | 32 (18 to 77) | 31 (17 to 51) | 38 (18 to 69) | 57 (37 to 78) | 41 (18 to 73) |
Sex female/male | 10/10 | 15/15 | 11/9 | 6/2 | 11/19 |
Phase of disease (Number of patients) | Acutea symptoms (20) | Acutea relapse (27), remission (3) | Acutea relapse (15), remission (5) | Acutea (6), chronicb (2) | Acutea (22), remission (2), unknown (6) |
CSF parametes
| |||||
CSF cell count per μl | 2 (1 to 5) | 7 (1 to 29) | 7 (0 to 81) | 106 (9 to 280) | 33 (2 to 512) |
QAlbumin
| 5 (3.0 to 9.6) | 5 (3.1 to 10.1) | 5 (3.3 to 7.5) | 12 (6.0 to 25.9) | 8 (3.7 to 55.5) |
Intrathecal IgG production | 0/20 | 17/30 | 13/18 | 7/8 | 10/29 |
Intrathecal IgA production | 0/20 | 0/30 | 2/18 | 5/8 | 9/29 |
Intrathecal IgM production | 0/20 | 4/30 | 5/18 | 6/8 | 6/29 |
OCBs (+/-) | 0/20 | (25/0)c
| (19/1) | (6/2) | (13/17) |
Impairment blood-CSF barrier | 2/18 | 8/30 | 3/20 | 6/8 | 21/30 |
Specimen handling and routine CSF testing
ELISA
Immunophenotyping
Statistical analysis
Results
Cytokine levels in the CSF
Immune cell subsets within the CSF of different patient cohorts
Correlation among chemokine/cytokine levels, CSF parameters and immune cell subsets
APRIL | BAFF | CXCL12 | CXCL13 | CCL19 | |
---|---|---|---|---|---|
Cell count |
P <0.0001
|
P <0.0001
|
P <0.0001
|
P <0.0001
|
P <0.0001
|
r = 0.4350
|
r = 0.4967
|
r = 0.4954
|
r = 0.7583
|
r = 0.5461
| |
Qalb
|
P = 0.0067
|
P = 0.0003
|
P <0.0001
|
P <0.0001
|
P <0.0001
|
r = 0.2669
|
r = 0.3507
|
r = 0.5011
|
r = 0.5066
|
r = 0.4600
| |
Intrathecal IgG |
P = 0.9332 |
P = 0.2370 |
P = 0.8147 |
P = 0.0002
|
P = 0.0808 |
r = 0.008406 | r = 0.1175 | r = -0.02337 |
r = 0.3540
| r = 0.1737 | |
Intrathecal IgA |
P = 0.0305
|
P = 0.0034
|
P = 0.0060
|
P <0.0001
|
P = 0.2992 |
r = 0.2143
|
r = 0.2863
|
r = 0.2692
|
r = 0.3965
| r = 0.1038 | |
Intrathecal IgM |
P = 0.0018
|
P = 0.0330
|
P = 0.0540 |
P = 0.0002
|
P = 0.5006 |
r = 0.3049
|
r = 0.2103
| r = 0.1904 |
r = 0.3567
| r = 0.06745 | |
OCBs |
P = 0.5013 |
P = 0.8673 |
P = 0.0971 |
P = 0.0104
|
P = 0.8844 |
r = -0.06767 | r = 0.01684 | r = -0.1660 |
r = 0.2539
| r = 0.01465 | |
All B cells |
P = 0.4474 |
P = 0.0478
|
P = 0.0312
|
P <0.0001
|
P = 0.0855 |
(CD19+) | r = 0.07422 |
r = 0.1918
|
r = 0.2084
|
r = 0.6264
| r = 0.1670 |
B cells |
P = 0.8110 |
P = 0.0945 |
P = 0.0859 |
P <0.0001
|
P = 0.2317 |
(CD19+D138-) | r = 0.02339 | r = 0.1625 | r = 0.1668 |
r = 0.5756
| r = 0.1166 |
Plasmablasts |
P = 0.0317
|
P = 0.0100
|
P = 0.0426
|
P <0.0001
|
P = 0.0067
|
(CD19+CD138+) |
r = 0.2078
|
r = 0.2481
|
r = 0.1965
|
r = 0.5828
|
r = 0.2605
|
T cells |
P = 0.7699 |
P = 0.1733 |
P = 0.0036
|
P = 0.5173 |
P = 0.0337
|
(CD3+) | r = -0.02931 | r = -0.1359 |
r = -0.2861
| r = -0.06485 |
r = -0.2105
|
Monocytes |
P = 0.0050
|
P <0.0001
|
P = 0.0034
|
P <0.0001
|
P = 0.0030
|
(CD14+) |
r = -0.2706
|
r = -0.3866
|
r = -0.2819
|
r = -0.5834
|
r = -0.2861
|
Stepwise multiple regression
Stepwise multiple regression | Predictor variables | ||||||
---|---|---|---|---|---|---|---|
Model
|
Variable
|
Beta
|
P
| ||||
F
|
df
|
P
|
R
2
| ||||
B cells all
| 21.650 | 3 | 0.000 | 0.391 | CXCL13 | 0.745 | 0.000 |
CXCL12 | −0.490 | 0.001 | |||||
BAFF | 0.320 | 0.012 | |||||
B cells
| 18.098 | 2 | 0.000 | 0.262 | CXCL13 | 0.616 | 0.000 |
CXCL12 | −0.271 | 0.010 | |||||
Plasmablasts
| 20.265 | 2 | 0.000 | 0.287 | CXCL13 | 0.413 | 0.000 |
BAFF | 0.234 | 0.009 | |||||
T cells
| 6.001 | 2 | 0.003 | 0.110 | BAFF | −0.498 | 0.001 |
APRIL | 0.296 | 0.05 | |||||
Monocytes
| No variables included |