Background
Methods
Cell lines and culture conditions
Recombinant Adenovirus and Infection
RT - PCR
Hybridization and analysis of microarrays data
Quantitative Reverse Transcription PCR (RT-qPCR)
Results
E2 expression in AdE2 infected C-33A cells
Differences in the transcriptional profiles
Gene ID | Symbol | Name | Fold Change |
---|---|---|---|
NM_017839 | LPCAT2 | Lysophosphatidylcholine acyltransferase 2 | 5.3 |
NM_002071 | GNAL | Guanine nucleotide binding protein (G protein) | 4.9 |
NM_018667 | SMPD3 | Sphingomyelin phosphodiesterase 3 | 4.7 |
NM_004283 | RAB3D | RAB3D, member RAS oncogene family | 4.5 |
NM_006327 | TIMM23 | Translocase of inner mitochondrial membrane 23 homolog (yeast) | 4.5 |
NM_017902 | HIF1AN | Hypoxia inducible factor 1 | 4.2 |
NM_001421 | ELF4 | E74-like factor 4 | 3.9 |
NM_023919 | TAS2R7 | Taste receptor, type 2, member 7 | 3.9 |
NM_004289 | NFE2L3 | Nuclear factor (erythroid-derived 2)-like 3 | 3.9 |
NM_003630 | PEX3 | Peroxisomal biogenesis factor 3 | 3.8 |
NM_025087 | CWH43 | Cell wall biogenesis 43 C-terminal homolog (S. cerevisiae) | 3.8 |
NM_013417 | IARS | Isoleucyl-tRNA synthetase | 3.8 |
NM_012338 | TSPAN12 | Tetraspanin 12 | 3.7 |
NM_004131 | GZMB | Granzyme B (granzyme 2) | 3.7 |
NM_002427 | MMP13 | Matrix metallopeptidase 13 (collagenase 3) | 3.6 |
NM_017849 | TMEM127 | Transmembrane protein 127 | 3.6 |
NM_003821 | RIPK2 | Receptor-interacting serine-threonine kinase 2 | 3.6 |
NM_003958 | RNF8 | Ring finger protein 8 | 3.5 |
NM_004224 | GPR50 | G protein-coupled receptor 50 | 3.5 |
NM_001244 | TNFSF8 | Tumor necrosis factor (ligand) superfamily, member 8 | 3.4 |
NM_020375 | C12orf5 | Chromosome 12 open reading frame 5 | 3.4 |
NM_004219 | PTTG1 | Pituitary tumor-transforming 1 | 3.4 |
NM_005925 | MEP1B | Meprin A, beta | 3.4 |
NM_024730 | RERGL | RERG/RAS-like | 3.3 |
NM_014942 | ANKRD6 | Ankyrin repeat domain 6 | 3.3 |
NM_032946 | NXF5 | Nuclear RNA export factor 5 | 3.3 |
NM_004528 | MGST3 | Microsomal glutathione S-transferase 3 | 3.3 |
NM_012280 | FTSJ1 | FtsJ homolog 1 (E. coli) | 3.3 |
NM_001372 | DNAH9 | Dynein, axonemal, heavy chain 9 | 3.2 |
NM_013256 | ZNF180 | Zinc finger protein 180 | 3.2 |
NM_003569 | STX7 | Syntaxin 7 | 3.2 |
NM_018370 | DRAM1 | DNA-damage regulated autophagy modulator 1 | 3.2 |
NM_017817 | RAB20 | RAB20, member RAS oncogene family | 3.2 |
NM_020377 | CYSLTR2 | Cysteinyl leukotriene receptor 2 | 3.2 |
NM_022087 | GALNT11 | N-acetylgalactosaminyltransferase 11 | 3.2 |
NM_004426 | PHC1 | Polyhomeotic homolog 1 (Drosophila) | 3.2 |
NM_031857 | PCDHA9 | Protocadherin alpha 9 | 3.2 |
NM_019863 | F8 | Coagulation factor VIII, procoagulant component | 3.1 |
NM_018840 | C20orf24 | Chromosome 20 open reading frame 24 | 3.1 |
NM_006243 | PPP2R5A | Protein phosphatase 2, regulatory subunit B', alpha isoform | 3.1 |
NM_017946 | FKBP14 | FK506 binding protein 14, 22 kDa | 3.1 |
NM_025212 | CXXC4 | CXXC finger 4 | 3.1 |
NM_012214 | MGAT4A | Mannosyl (alpha-1,3-)-glycoprotein beta-1,4-N-acetylglucosaminyltransferase, isozyme A | 3.1 |
NM_012087 | GTF3C5 | General transcription factor IIIC, polypeptide 5, 63 kDa | 3.1 |
NM_017952 | PTCD3 | Pentatricopeptide repeat domain 3 | 3.1 |
NM_005967 | NAB2 | NGFI-A binding protein 2 (EGR1 binding protein 2) | 3.1 |
NM_016021 | UBE2J1 | Ubiquitin-conjugating enzyme E2, J1 (UBC6 homolog, yeast) | 3.1 |
NM_014945 | ABLIM3 | Actin binding LIM protein family, member 3 | 3.1 |
NM_001006 | RPS3A | Ribosomal protein S3A | 3.1 |
NM_012200 | B3GAT3 | Beta-1,3-glucuronyltransferase 3 (glucuronosyltransferase I) | 3.1 |
Gene ID | Symbol | Name | Fold Change |
---|---|---|---|
NM_001872 | CPB2 | Carboxypeptidase B2 | -8.5 |
NM_024610 | HSPBAP1 | HSPB (heat shock 27 kDa) associated protein 1 | -8.2 |
NM_022118 | RBM26 | RNA binding motif protein 26 | -8.2 |
NM_017819 | RG9MTD1 | RNA (guanine-9-) methyltransferase domain containing 1 | -8.1 |
NM_005319 | HIST1H1C | histone cluster 1, H1c | -7.9 |
NM_000859 | HMGCR | 3-hydroxy-3-methylglutaryl-Coenzyme A reductase | -7.9 |
NM_003829 | MPDZ | Multiple PDZ domain protein | -7.8 |
NM_017806 | LIME1 | Lck interacting transmembrane adaptor 1 | -7.5 |
NM_005166 | APLP1 | Amyloid beta (A4) precursor-like protein 1 | -7.5 |
NM_015985 | ANGPT4 | Angiopoietin 4 | -7.3 |
NM_016283 | TAF9 | TAF9 RNA polymerase II, TATA box binding protein (TBP)-associated factor | -7.0 |
NM_000808 | GABRA3 | Gamma-aminobutyric acid (GABA) A receptor, alpha 3 | -7.0 |
NM_005264 | GFRA1 | GDNF family receptor alpha 1 | -7.0 |
NM_003420 | ZNF35 | Zinc finger protein 35 | -7.0 |
NM_016179 | TRPC4 | Transient receptor potential cation channel, subfamily C, member 4 | -6.9 |
NM_020200 | PRTFDC1 | Phosphoribosyl transferase domain containing 1 | -6.9 |
NM_014736 | KIAA0101 | KIAA0101 | -6.9 |
NM_020651 | PELI1 | Pellino homolog 1 (Drosophila) | -6.8 |
NM_006061 | CRISP3 | Cysteine-rich secretory protein 3 | -6.8 |
NM_014626 | TAAR2 | Trace amine associated receptor 2 | -6.8 |
NM_007048 | BTN3A1 | Butyrophilin, subfamily 3, member A1 | -6.7 |
NM_007213 | PRAF2 | PRA1 domain family, member 2 | -6.7 |
NM_024838 | THNSL1 | Threonine synthase-like 1 (S. cerevisiae) | -6.7 |
NM_012198 | GCA | Grancalcin, EF-hand calcium binding protein | -6.6 |
NM_018938 | PCDHB4 | Protocadherin beta 4 | -6.6 |
NM_006530 | YEATS4 | YEATS domain containing 4 | -6.4 |
NM_000500 | CYP21A2 | Cytochrome P450, family 21, subfamily A, polypeptide 2 | -6.4 |
NM_014547 | TMOD3 | Tropomodulin 3 (ubiquitous) | -6.4 |
NM_000228 | LAMB3 | Laminin, beta 3 | -6.3 |
NM_002029 | FPR1 | Formyl peptide receptor 1 | -6.3 |
NM_016508 | CDKL3 | Cyclin-dependent kinase-like 3 | -6.3 |
NM_017779 | DEPDC1 | DEP domain containing 1 | -6.3 |
NM_024612 | DHX40 | DEAH (Asp-Glu-Ala-His) box polypeptide 40 | -6.3 |
NM_020666 | CLK4 | CDC-like kinase 4 | -6.3 |
NM_007029 | STMN2 | Stathmin-like 2 | -6.2 |
NM_012095 | AP3M1 | Adaptor-related protein complex 3, mu 1 subunit | -6.2 |
NM_018319 | TDP1 | Tyrosyl-DNA phosphodiesterase 1 | -6.2 |
NM_024665 | TBL1XR1 | Transducin (beta)-like 1 X-linked receptor 1 | -6.2 |
NM_012123 | MTO1 | Mitochondrial translation optimization 1 homolog (S. cerevisiae) | -6.2 |
NM_001285 | CLCA1 | Chloride channel accessory 1 | -6.2 |
NM_025074 | FRAS1 | Fraser syndrome 1 | -6.2 |
NM_017424 | CECR1 | Cat eye syndrome chromosome region, candidate 1 | -6.1 |
NM_024756 | MMRN2 | Multimerin 2 | -6.1 |
NM_002492 | NDUFB5 | NADH dehydrogenase (ubiquinone) 1 beta subcomplex, 5 | -6.1 |
NM_004549 | NDUFC2 | NADH dehydrogenase (ubiquinone) 1, subcomplex unknown, 2 | -6.1 |
NM_018128 | TSR1 | TSR1, 20S rRNA accumulation, homolog (S. cerevisiae) | -6.0 |
NM_000438 | PAX3 | Paired box 3 | -6.0 |
NM_018991 | STAG3L1 | Stromal antigen 3-like 1 | -6.0 |
NM_024576 | OGFRL1 | Opioid growth factor receptor-like 1 | -6.0 |
NM_004249 | RAB28 | RAB28, member RAS oncogene family | -6.0 |
Cellular Pathway | No. genes altered |
---|---|
Wnt signaling pathway
| 15 |
Inflammation mediated by chemokine and cytokine signaling pathway
| 10 |
Angiogenesis
| 8 |
Integrin signalling pathway
| 8 |
Cadherin signaling pathway
| 8 |
B cell activation
| 8 |
Apoptosis signaling pathway
| 7 |
PDGF signaling pathway
| 7 |
EGF receptor signaling pathway
| 7 |
Oxidative stress Response
| 6 |
Cellular Pathway | No. genes altered |
---|---|
Wnt signaling pathway
| 28 |
PDGF signaling pathway
| 22 |
Angiogenesis
| 20 |
Inflammation mediated by chemokine and cytokine signaling pathway
| 20 |
Integrin signalling pathway
| 16 |
TGF-beta signaling pathway
| 13 |
p53 pathway
| 12 |
FGF signaling pathway
| 11 |
Apoptosis signaling pathway
| 10 |
PI3 kinase pathway
| 10 |