Background
Results
Macrophages infiltrate into the DRG after peripheral nerve injury
TLR2 contributes to macrophage infiltration in the DRG after peripheral nerve injury
Peripheral nerve injury-induced expressions of CCL2/MCP-1 and CCL3/MIP-1α in the DRG are reduced in TLR2 knock-out mice
DRG | CCL2 | CCL3 | CCL5 | CXCL1 | CXCL10 |
---|---|---|---|---|---|
6 h
|
20.9 ± 3.6* |
8.0 ± 1.6* |
0.7 ± 0.3 |
5.8 ± 0.5** |
0.6 ± 0.2 |
24 h
|
7.0 ± 0.9* |
4.2 ± 0.2** |
0.8 ± 0.0 |
2.2 ± 0.4* |
1.1 ± 0.3 |
48 h
|
4.1 ± 0.2** |
4.1 ± 0.5* |
2.5 ± 0.2* |
2.7 ± 0.1** |
2.9 ± 0.6 |
TLR2 contributes to peripheral nerve injury-induced TNF-α and IL-1β expression in the DRG and to the development of spontaneous pain behavior
Discussion
Conclusions
Methods
Animals and Surgery
Behavioral testing
Real-time RT-PCR
Genes | Forward primers | Reverse primers | GenBank no. |
---|---|---|---|
mouse GAPDH | 5'-AGG TCA TCC CAG AGC TGA ACG-3' | 5'-CAC CCT GTT GCT GTA GCC GTA T-3' | NM_008084 |
mouse CD11b | 5'-TAA TGA CTC TGC GTT TGC CCT G-3' | 5'-ATT GGA GCT GCC CAC AAT GAG-3' | NM_008401 |
mouse TNF-α | 5'-AGC AAA CCA CCA AGT GGA GGA-3' | 5'-GCT GGC ACC ACT AGT TGG TTG T-3' | NM_013693 |
mouse IL-1β | 5'-TTG TGG CTG TGG AGA AGC TGT-3' | 5'-AAC GTC ACA CAC CAG CAG GTT-3' | NM_008361 |
mouse CCL2 | 5'-TCA GCC AGA TGC AGT TAA CG-3' | 5'-GAT CCT CTT GTA GCT CTC CAG C-3' | BC05507 |
mouse CCL3 | 5'-ACT GCC TGC TGC TTC TCC TAC A-3' | 5'-AGG AAA ATG ACA CCT GGC TGG-3' | BC111443 |
mouse CCL5 | 5'-CTC ACC ATC ATC CTC ACT GCA-3' | 5'-GCA CTT GCT GCT GGT GTA GAA A-3' | AY722103 |
mouse CXCL1 | 5'-CAC ACT CAA GAA TGG TCG CGA-3' | 5'-TTG TCA GAA GCC AGC GTT CAC-3' | BC132502 |
mouse CXCL10 | 5'-TGC CGT CAT TTT CTG CCT CA-3' | 5'-TCA CTG GCC CGT CAT CGA TAT-3' | BC030067 |