There are two types of p53 genes: the wild type p53 gene and the mutant p53 gene. The wild type p53 gene, which is also known as the guardian of gene, is indispensable to regulate the normal cells circle [
13,
14]. On the one hand, as the important regulating factor in the process of apoptosis, the wild type p53 monitor the integrity of genes all the time, on the other hand, as the nuclear transcripton, it can respectively combines with DNA and RNA polymerase to regulate expression of gene, it can also inhibit the synthesis of DNA, take part in the repair of DNA, induce cell growth stop at the phase of G0. In addition, the wild type p53 gene can induce the Fas-mediated cell procedural death after the damage of DNA, so that the regular growth of cell is maintained. Wang JJ [
8] had found, while discussing the anticancer mechanism of KLT injection, that the labeling index of wild type p53 in treat group which received KLT injection is 16.8%, while the control group had no expression at all. Furthermore, in the experiment about KLT-induced apoptosis, Bao Y [
15] discovered that compared with the control group, the mRNA level of the wild type p53 gene significantly Increased in 20 μl/ml KLT experimental group after 48 hours. And in the experiment about apoptosis of multidrug resistance phenotypic human breast cancer cell line MCF7
adr and its cell cycle arrest that induced by KLT. According to the immunohistochemical detection, Guo JW [
16] found that the wild type p53 gene of MCF7
adr in the control group is negative, but the wild type p53 gene of MCF7
adr in the KLT experimental group is midrange positive, it can be assumed that KLT could up-regulate the expression of p53 and extend half life of p53 protein. In addition, Wei CY [
12] observed 34 cases' hepatoma carcinoma cell that cultivated in vitro and their changes after treated with KLT, the results indicated that, compared with the control group, the apoptosis of hepatoma carcinoma cell in KLT experimental group is very significant, there was significant difference between them, at the same time, the labeling index of wild type p53 in the treat group is (8.39 ± 1.42)%, but the labeling index of wild type p53 in the control group (2.11 ± 0.97)%, there was significant difference between these two groups. the phenomenon recorded here is the same as the studies of Wang JJ, Guo JW and Bao Y which had indicated [
8,
15,
16]. In conclusion, KLT injection's may induce the apoptosis of tumor cell by way of up-regulate the expression of p53 genes.