Higher serum soluble receptor for advanced glycation end product levels and lower prevalence of metabolic syndrome among Japanese adult men: a cross-sectional study

The receptor for advanced glycation end products (RAGE) is a cell surface molecule belonging to the immunoglobulin superfamily that binds many ligands including advanced glycation end products (AGEs) [1]. Circulating forms of RAGE, arising from receptor ectodomain shedding [soluble RAGE (sRAGE)] and secretion of its splice variant [endogenous secretory RAGE (esRAGE)], may competitively inhibit binding of the ligand to membrane-bound RAGE by acting as an endogenous decoy [2]. Indeed, in animal models, recombinant sRAGE administration suppressed the development of atherosclerosis and stabilized established atherosclerosis [3, 4]. Thus, circulating RAGE (sRAGE and esRAGE) levels may inversely reflect ligand − RAGE interaction evoked pathogenesis.

Several studies have reported that decreased circulating RAGE concentrations are associated with metabolic syndrome (MetS) risk factors [5‐9]. Moreover, participants with MetS showed significantly lower plasma esRAGE concentration than those without MetS [7], and plasma sRAGE levels decreased with increasing number of MetS risk factors [10]. Proinflammatory state [e.g., high concentration of C-reactive protein (CRP)] is considered to have a significant impact on the pathogenesis of MetS and its components [11]. Under low grade chronic inflammation circumstances, higher sRAGE level may attenuate the deteriorating effect of inflammation on MetS and its components. However, such association has not been investigated.

Thus, the purpose of this study was to examine the association between circulating RAGE (sRAGE and esRAGE) concentrations and the prevalence of MetS in Japanese adult in a population-based cross-sectional study, with particular regard to systemic inflammatory level measured by circulating CRP.

Of the 712 men, 179 (25.1%) had ≥3 MetS components. The median (interquartile range) values were 1259.0 (946.4–1609.1) pg/mL for serum sRAGE, 319.0 (235.7–432.2) pg/mL for serum esRAGE, and 0.38 (0.22–0.77) mg/L for hsCRP. Among women, 24 (13.6%) had ≥3 MetS components. The median (interquartile range) values were 1547.9 (1139.6–1922.0) pg/mL for serum sRAGE, 358.1 (259.3–461.5) pg/mL for serum esRAGE, and 0.23 (0.12–0.46) mg/L for hsCRP. The concentration of serum sRAGE was strongly correlated with the concentration of serum esRAGE (Figure 2).

The characteristics according to the tertiles of serum sRAGE in men are presented in Table 1. Serum sRAGE concentration was negatively associated with age, BMI, WC, SBP, DBP, hsCRP (P for trend < 0.001), and TG (P for trend = 0.036). Moreover, higher tertiles of serum sRAGE had a lower percentage of participants with ≥23 METs × hours/week (P for trend = 0.011) and those who drank 7 days/week (P for trend < 0.001), and they also had a higher percentage of current smokers (P for trend = 0.020). No other significant associations were observed between the groups. Among women, serum sRAGE concentration was negatively associated with BMI, WC, SBP, and hsCRP (Additional file 1: Table S1).

The prevalence of MetS was 30.7% in the lowest tertile of serum sRAGE, 27.4% in the middle tertile, and 17.3% in highest tertile (P for trend = 0.001, Table 1) among men. Moreover, higher serum sRAGE concentration was negatively associated with the number of MetS components (P for trend < 0.001), and negatively associated with the prevalence of central obesity and elevated BP (P for trend < 0.001). Similar results were obtained for women (Additional file 1: Table S1).

Table 2 shows the relationship of tertiles of serum sRAGE with the prevalence of MetS and its components after adjustment for potential confounders in men. The adjusted odds ratio (95% confidence interval) for MetS in the middle and highest tertiles of serum sRAGE were 0.89 (0.59–1.35) and 0.45 (0.28–0.71) (P for trend = 0.001), respectively. Even after adjustment for serum hsCRP concentration, participants with higher serum sRAGE concentration had a lower prevalence of MetS [1.04 (0.67–1.61) for middle tertile, and 0.58 (0.36–0.95) for highest tertile, P for trend = 0.038]. Furthermore, participants with higher serum sRAGE concentration had a lower prevalence of central obesity [0.86 (0.58–1.27) for middle tertile, 0.33 (0.21–0.52) for highest tertile, P for trend < 0.001], elevated BP [0.61 (0.40–0.93) for middle tertile, 0.38 (0.25–0.57) for highest tertile, P for trend < 0.001], and elevated TG [1.00 (0.68–1.48) for middle tertile, and 0.62 (0.41–0.93) for highest tertile, P for trend = 0.024], after adjustment for potential confounders. With central obesity and elevated BP, even after adjustment for serum hsCRP concentration (model 2) and mutual MetS components (model 3), the association remained. Moreover, similar results were obtained for the relationships of serum esRAGE concentration with MetS and its components, except for reduced HDL-C in model 1 [0.98 (0.58–1.65) for middle tertile, and 0.44 (0.24–0.79) for highest tertile, P for trend = 0.006]. Among women, serum sRAGE level was associated with only elevated BP after adjustment for confounding factors (Additional file 1: Table S2).

We examined the interrelationship between sRAGE, MetS, and hsCRP. Table 3 shows the adjusted odds ratio (95% confidence intervals) for MetS in the higher hsCRP/higher sRAGE, lower hsCRP/lower sRAGE, and lower hsCRP/higher sRAGE categories in men. Participants in the higher hsCRP/higher sRAGE category had a 40% lower odds ratio for MetS than those in the higher hsCRP/lower sRAGE category (P = 0.031). Moreover, after adjustment for mutual MetS components (model 2), participants in the higher hsCRP/higher sRAGE category had a 46% lower odds ratio for central obesity (P = 0.020) and a 51% lower odds ratio for elevated BP (P = 0.007) than those in the higher hsCRP/lower sRAGE category. Among women, there was no difference between higher hsCRP/higher sRAGE and hsCRP/lower sRAGE category after adjustment for confounders (Additional file 1: Table S3).

To examine the influence of those excluded because of incomplete data, we examined the associations between circulating sRAGE or esRAGE levels and the prevalence of MetS including those who had incomplete data by complementing the incomplete data. For example, the educational level was categorized into < college, ≥college, or missing value group, and physical activity was categorized into <1.0, 1–22.9, ≥23.0 METs · hours/week, or missing value group. For continuous variables including eGFR and energy intake, missing values were assigned to their medians. In model 2, for men, the adjusted odds ratio (95% confidence interval) for MetS in the middle and highest tertiles of serum sRAGE were 0.83 (0.56–1.26) and 0.46 (0.29–0.72) (P for trend = 0.001), respectively. Among women, even inclusion of participants with incomplete data, there was no significant association between serum sRAGE and esRAGE, and MetS. Moreover, for the interrelationship between sRAGE, MetS, and hsCRP among men, participants in the higher hsCRP/higher sRAGE category had a 40% lower odds for MetS than those in the higher hsCRP/lower sRAGE category (0.59 [0.38–0.92], P = 0.021).

The present study examined the association between the levels of circulating RAGE (sRAGE and esRAGE) and prevalence of MetS and its component among adult with low grade inflammatory level, through a population-based cross-sectional study. Our results suggested that, among men, after adjustment for serum hsCRP concentration higher circulating RAGE levels were associated with lower prevalence of MetS, central obesity, and elevated BP. Moreover, participants in the higher hsCRP/higher sRAGE category had lower odds ratio of MetS than those in the higher hsCRP/lower sRAGE category. Thus, higher levels of circulating RAGE were associated with lower prevalence of MetS and its components including central obesity and elevated BP among adult men with low grade inflammation.

Previous studies have reported a relationship between circulating RAGE level and MetS [7, 10]. For example, Koyama et al. reported that participants with MetS had lower circulating esRAGE concentration than those without it, using a univariate model among type 2 diabetic patients and healthy controls [7]. Recently, in young to middle-aged medication-free non-diabetic subjects, plasma sRAGE concentration was found to decrease as the number of MetS risk factors increased [10]. In the present study, we focused on the relationship between circulating RAGE level and the prevalence of MetS because (i) a proinflammatory state, such as high concentration of circulating CRP, was reported to significantly influence the pathogenesis of MetS and its components [11], and (ii) circulating soluble forms of RAGE may attenuate inflammatory responses via competitive inhibition of ligand-RAGE interaction [2]. However, our finding showed that the significant negative association between serum sRAGE level and the prevalence of MetS remained after adjusting for serum hsCRP level. Therefore, circulating sRAGE as an anti-inflammatory decoy may not be sufficient to explain the contributions of circulating sRAGE to the lower prevalence of MetS. In addition, in the present study, the mean (SD) serum sRAGE concentration was 1344.8 (563.9) pg/ml. Although the concentrations of circulating ligands for RAGE were not measured in this study, a previous study of Japanese adult men reported that the concentration of circulating high mobility group box 1, a RAGE ligand, was 1.69 (0.04) ng/ml [21]. As RAGE is known to be a multi-ligand receptor, the potential ligand concentration likely exceeds the sRAGE concentration. Therefore, it remains unknown whether the concentration of circulating soluble forms of RAGE is sufficient to scavenge accumulating ligands [22].

One of the potential mechanisms explaining the contribution of sRAGE to the decreased incidence of MetS is polymorphisms in RAGE. Kankova et al. [23] demonstrated that subjects bearing the 1704 T allele of the RAGE gene had significantly lower plasma levels of antioxidants, including carotenoids, tocopherol, lutein, and lycopene, than those with the 1704G allele. These results suggest that G1704T is involved in oxidative stress. A recent meta-analysis indicated that the frequency of the 1704 T allele in East Asian populations was 7.76–20.6%, which is substantially higher than that in Caucasians (4.82–9.92%); moreover, the ORs associating the 1704 T allele with diabetes and its complications are higher in East Asian populations [24]. Moreover, RAGE single-nucleotide polymorphism rs2060700 (Gly82Ser) is strongly associated with circulating sRAGE levels [25]. Furthermore, the RAGE Gly82Ser polymorphism is associated with a risk of coronary artery disease [26]. In addition, another RAGE polymorphism might be linked with insulin resistance [27]. Given these results, further comprehensive studies including the evaluation of RAGE polymorphisms are required to clarify the association between circulating soluble forms of RAGE and MetS.

Recently, the role of circulating sRAGE is conflicting. Colhoun et al. investigated the relationship of sRAGE to incident coronary heart disease (CHD) in patients with type 2 diabetes [28]. The patients were followed for 3.9 years, and it was demonstrated that sRAGE concentration were positively associated with incident CHD in type 2 diabetes [28]. Similar results have been obtained among type 1 diabetes [29, 30] and elderly women [31]. These results suggest that serum sRAGE concentration has a bivalent role. In conditions of elevated inflammatory level, such as diabetes or aging, serum sRAGE may be a marker of inflammation rather than a decoy, because there is emerging evidence that proteolytic cleavage, through which the component is formed, is part of a regulatory process and may reflect ongoing inflammation [32]. One would, therefore, expect higher concentration to be associated with more vascular disease. In contrast, among our participants, higher sRAGE concentration was associated with a lower prevalence of MetS even if they were in the higher hsCRP category. Consistent with our results, Selvin et al. have demonstrated a negative relationship between low concentration of circulating sRAGE and the risks of diabetes, CHD, and mortality in a community-based prospective cohort of middle-aged adults [33]. Thus, these results suggested that serum sRAGE concentration may be a marker of inflammation in conditions of elevated CRP, whereas they may be negatively associated with a risk of cardiovascular diseases and its risk factors, such as MetS, in conditions of low grade inflammation.

Although the details of regulation of soluble forms of RAGE have not been revealed, it is possible that the risk factors of MetS or cardiovascular diseases might influence the amount of circulating soluble forms of RAGE. Previous studies have reported the relationships between higher serum sRAGE concentration and lower BMI and WC in general population [5] and non-diabetic Japanese populations [6], and consistent with this findings, there was a positive correlation with adiponectin [28]. We also showed that higher sRAGE concentration was associated with lower prevalence of central obesity in this study. Thus, although the mechanism of the association between lower level of circulating soluble forms of RAGE and obesity is not still clear, the impaired function of adipocyte might be contributing to lower level of circulating soluble forms of RAGE. An alternative possibility is an up-regulation of full-length RAGE shedding by treatment of statin. A recent in vitro study showed that reduction of cellular cholesterol by statins significantly increases the levels of soluble RAGE by enhancement of full-length RAGE shedding [34]. This result was supported by clinical studies with hypercholesterolemic [35]. Further experimental studies are necessary to elucidate the regulation of soluble forms of RAGE.

In addition to central obesity, there was a negative association between the prevalence of elevated BP and serum sRAGE levels. Geroldi et al. showed that the plasma concentration of sRAGE was lower in hypertensive subjects than in normotensive controls [9]. Because crosslinking between collagen molecules and AGE could be implicated in the pathogenesis of arterial stiffening and hypertension [36], the secreted form of RAGE could prevent hypertension by binding to circulating AGE, thus preventing them from forming protein–protein crosslinks. A significantly elevated AGE concentration was found in the vascular smooth muscle cells of spontaneously hypertensive rats [37]. Taken together, higher circulating sRAGE concentrations may be associated with lower the prevalence of MetS by lowering the prevalence of central obesity and elevated BP.

There are some limitations in this study. First, because the sample size of our female participants was relatively small, statistical power may not have been sufficient to obtain statistical significance. Whether the abovementioned relationship is present in women populations remains unknown. Second, because this study used a cross-sectional design, we cannot conclude the causal relationship between sRAGE and esRAGE and the prevalence of MetS and its components. A larger population-based prospective study needs to be performed to further confirm the causal relationship between sRAGE and esRAGE and the prevalence of MetS and its components.

In conclusion, higher concentrations of circulating RAGE were associated with lower prevalence of MetS and its components including central obesity and elevated BP among Japanese adult men with low grade inflammation.