Background
Gene-symbol
| Aliases | mRNA ID |
---|---|---|
ABC transporters
| ||
Abcb1b
| Mdr1, Pgy1, Abcb1 | NM_012623 |
Abcc1
| Mrp1 | NM_022281 |
Abcc3
| Mlp2, Mrp3 | NM_080581 |
Abcc4
| Mrp4 | NM_133411 |
Abcc5
| Mrp5, Abcc5a | NM_053924 |
Abcc9
| Sur2 | NM_013040 |
Abcc10
| Mrp7 | NM_001108201 |
Abcg2
| BCRP1 | NM_181381 |
Solute carriers: organic anion transporting polypeptides
| ||
Slco2a1
| Slc21a2, Matr1 | NM_022667 |
Slco1a3
| Slc21a4 | NM_030837 |
Slco1a4
| Slc21a5, Oatp2 | NM_131906 |
Slco1a5
| Slc21a7, Oatp3 | NM_030838 |
Slco2b1
| Slc21a9, moat1 | NM_080786 |
Slco3a1
| Slc21a11, Oatp3a1 | NM_177481 |
Slco1c1
| Bsat1, Slc21a14, Oatp14 | NM_053441 |
Solute carriers: organic anion /cation/zwitterion transporters
| ||
Slc22a2
| OCT2 | NM_031584 |
Slc22a4
| Octn1 | NM_022270 |
Slc22a5
| Octn2, CT1, UST2r | NM_019269 |
Slc22a6
| Oat1, Paht, Roat, Orctl1 | NM_017224 |
Slc22a7
| Oat2 | NM_053537 |
Slc22a8
| Oat3, Roct | NM_031332 |
Slc22a12
| Rst | NM_001034943 |
Slc22a15
| NM_001107707 | |
Slc22a17
| Boct, 24p3R | NM_177421 |
Slc22a25
| Ust1, Slc22a9 | NM_138908 |
Solute carriers: nucleoside and peptide transporters
| ||
Slc15a2
| Pept2 | NM_031672 |
Slc28a2
| Cnt2 | NM_031664 |
Slc28a3
| Cnt3 | NM_080908 |
Slc29a1
| Ent1 | NM_031684 |
Slc29a2
| Ent2 | NM_031738 |
Slc29a3
| Ent3 | NM_181639 |
Slc29a4
| NM_001105911 | |
Cytochrome P450s, epoxide hydrolases, flavin-containing monooxygenases
| ||
Cyp1b1
| NM_012940 | |
Cyp2d4
| Cyp2d6, Cyp2d18 Cyp2d22 | NM_138515 |
Cyp2j4
| CYP2J2 | NM_023025 |
Cyp2u1
| NM_001024779 | |
Ephx1, transcript variant 1 | MEH8 | NM_001034090 |
Ephx1, transcript variant 2 | NM_012844 | |
Ephx2
| CEH, SEH | NM_022936 |
Fmo1
| RFMO1A | NM_012792 |
Fmo2
| NM_144737 | |
Fmo3
| NM_053433 | |
Fmo4, transcript variant 1 | NM_144562 | |
Fmo4, transcript variant 2 | NM_144561 | |
Fmo5
| NM_144739 | |
UDP-glucuronosyltransferases and sulfotransferases transferases
| ||
Ugt1a1
| UDPGT 1-1 | NM_012683 |
Ugt1a2
| UDPGT 1-2 | NM_201423 |
Ugt1a3
| UDPGT 1-3 | NM_201424 |
Ugt1a5
| UDPGT 1-5 | NM_001039549 |
Ugt1a6
| UDPGT 1-6 | NM_057105 |
Ugt1a7c
| UDPGT 1-7 | NM_130407 |
Ugt1a8
| UDPGT 1-8 | NM_175846 |
Ugt1a9
| NM_201425 | |
Ugt2a1
| Ugt2a1p | NM_022228 |
Sult1a1
| Stm, Stp1, ASTIV, Mx-ST, PST-1 | NM_031834 |
Sut1d1
| Sultn, Sult-n | NM_021769 |
Sult5a1
| NM_001106194 | |
Glutathione S-transferases (GSTs) and glutathione-synthesis related genes
| ||
Gsta1
| NM_017013 | |
Gsta2
| LOC494499 | |
Gsta3
| Yc1 | NM_031509 |
Gsta4
| NM_001106840 | |
Gstm1
| NM_017014 | |
Gstm2
| NM_177426 | |
Gstm3
| GstYb4 | NM_020540 |
Gstm4
| NM_001024304 | |
Gstm5
| NM_172038 | |
Gstm7
| NM_031154 | |
Gstp1
| Gst3, Gstp, Gstp2 | NM_012577 |
Mgst1
| NM_134349 | |
Mgst2
| NM_001106430 | |
Mgst3
| NM_001191594 | |
Gss
| NM_012962 | |
Gclc
| Glclc | NM_012815 |
Gclm
| Glclr | NM_017305 |
Heme oxygenases, biliverdin reductase
| ||
Hmox1
| Ho1, Heox, Hmox, Ho-1, HEOXG | NM_012580 |
Hmox2
| Ho-2 | NM_024387 |
Hmox3
| HO-3 | AF058787 |
Blvra
| NM_053850 | |
Blvrb
| NM_001106236 | |
Glutathione peroxidases and –reductase
| ||
Gpx1
| GSHPx, GSHPx-1 | NM_030826 |
Gpx3
| Gpxp, GPx-P, GSHPx-3, GSHPx-P | NM_022525 |
Gpx4
| Phgpx, gpx-4, snGpx | NM_017165 |
Gpx7
| NM_001106673 | |
Gpx8
| NM_001106411 | |
Gsr
| NM_053906 | |
Sod1
| CuZnSOD | NM_017050 |
Sod2
| NM_017051 | |
Sod3
| NM_012880 | |
Cat
| CS1, Cas1,Catl, Cs-1, Cat01 | NM_012520 |
Regulatory transcription factors
| ||
Nr1i2
| PXR | NM_052980 |
Nr1i3
| CAR | NM_022941 |
Rxra
| NM_012805 | |
Ppara
| Nr1c1, Ppar | NM_013196 |
Nr3c1
| GR, Grc, Grl | NM_012576 |
Cebpb
| LAP, TCF5, Il6dbp, NF-IL6 | NM_024125 |
Ahr
| AhR | NM_013149 |
Arnt
| Arnt1 | NM_012780 |
Nfe2l2
| Nrf2 | NM_031789 |
Hif1a
| MOP1 | NM_024359 |
Tp53
| p53, Trp53 | NM_030989 |
Monoamine oxidases, dopa decarboxylase, catechol-O-methyltransferase
| ||
Maoa
| Mao | NM_033653 |
Maob
| NM_013198 | |
Ddc
| NM_012545 | |
Comt
| NM_012531 | |
Tight junction-associated genes
| ||
Cldn1
| NM_031699 | |
Cldn2
| NM_001106846 | |
Cldn3
| NM_031700 | |
Cldn6
| NM_001102364 | |
Cldn9
| NM_001011889 | |
Cldn10
| NM_001106058 | |
Cldn11
| NM_053457 | |
Cldn12
| NM_001100813 | |
Cldn19
| NM_001008514 | |
Cldn22
| NM_001110143 | |
Marveld1
| Mrvldc1 | NM_001107590 |
Marveld2
| Mrvldc2, Tric | NM_001108936 |
Marveld3
| NM_001109132 | |
Ocln
| NM_031329 | |
Lsr
| Lisch7 | NM_032616 |
Tjp1
| ZO-1 | NM_001106266 |
Tjp2
| ZO-2 | NM_053773 |
Tjp3
| ZO-3 | NM_001108073 |
F11r
| Jam1 | NM_053796 |
Jam2
| NM_001034004 |
Materials and methods
Tissue collection and RNA extraction
Affymetrix microarray
Illumina RNA-Seq
Quantitative real-time PCR
Data presentation
Results and discussion
Combined analysis of gene expression data by Affymetrix microarray, Illumina RNA-sequencing, and qRT-PCR
Developmental profile of TJ-associated genes
Developmental profile of efflux transporters
ABC transporters
Slco transporters
Slc22 transporters
Slc15, Slc28 and Slc29 transporters
Developmental profile of drug-metabolizing and antioxidant enzymes
Phase I drug metabolizing enzymes
Phase II drug metabolizing enzymes
Antioxidant enzymes
Transcription factors involved in the regulation of detoxifying genes
Developmental profile of enzymes forming a barrier to neurotransmitters
General comments
TJ proteins | Transporters | Enzymes | |
---|---|---|---|
E15
| 5.9 ± 1.4 | 23.5 ± 9.2 | 5.1 ± 1.1 |
E19
| 2.5 ± 0.6 | 3.6 ± 0.9 | 2.1 ± 0.2 |
P2
| 1.9 ± 0.3 | 3.2 ± 0.7 | 1.9 ± 0.2 |
E15 vs. E19
| * | * | ** |
E15 vs. P2
| ** | ** | *** |
E19 vs. P2
| ns | ns | ns |