Background
Methods
The Cancer Genome Atlas (TCGA) data
Patient samples
Tissue Microarrays (TMAs) and IHC
PPP2R2A (B55α) scoring
Statistical analysis
Results
PPP2R2A CNA status is associated with mRNA expression levels and OS in the TCGA dataset
PPP2R2A (B55α)-/low expression is associated with higher grade and ER positive carcinomas
PPP2R2A (B55α) expression defines luminal-like BC groups with distinctive outcomes
PPP2R2A (B55α) -/low /Cyclin D1high expression defines a group of luminal BC with worse outcomes
Parameter | Status | Total n=227 | PPP2R2A (B55α)-/lowCyclin D1highn=33 (%) | Others n=194 (%) |
p
|
---|---|---|---|---|---|
Age | ≤ 50 | 91 | 14 (15.38) | 77 (84.62) | .767 |
> 50 | 136 | 19 (13.97) | 117 (86.03) | ||
n/a | - | ||||
Size | ≤ 2 cm | 120 | 18 (15.00) | 102 (85.00) | .834 |
> 2 cm | 107 | 15 (14.02) | 92 (85.98) | ||
n/a | - | ||||
Grade | I | 63 | 3 (4.76) | 60 (95.24) | .014 |
II | 108 | 17 (15.74) | 91 (84.26) | ||
III | 55 | 13 (23.64) | 42 (76.36) | ||
n/a | 1 | 1 | |||
Lymph node status | LNN | 105 | 14 (13.33) | 91 (86.67) | .306 |
Node pos. | 103 | 18 (17.48) | 85 (82.52) | ||
n/a | 19 | 1 | 18 | ||
PgR | Positive | 90 | 11 (12.22) | 79 (87.78) | .615* |
Negative | 10 | 2 (20.00) | 8 (80.00) | ||
n/a | 127 | 78 | 51 | ||
HER2 | Positive | 29 | 9 (31.03) | 20 (68.97) | .020* |
Negative | 198 | 24 (12.12) | 174 (87.88) | ||
n/a | - | ||||
KI-67 Index | <14 | 152 | 18 (11.84) | 134 (88.16) | .125 |
≥14 | 60 | 12 (20.00) | 48 (80.00) | ||
n/a | 15 | 3 | 12 | ||
Luminal subtype | A-like | 152 | 17 (11.18) | 135 (88.82) | .041 |
B-like | 75 | 16 (21.33) | 59 (78.67) | ||
n/a | - | ||||
DFS | Num. of events | 41 | 15 | 26 | .002# |
Mean (months) | 91 | 92 | 89 | ||
OS | Num. of events | 30 | 9 | 21 | .001# |
Mean (months) | 95 | 95 | 95 |
OS | DFS | ||||||||
---|---|---|---|---|---|---|---|---|---|
Univariate | Multivariate | Univariate | Multivariate | ||||||
Variable | HR (95% CI) |
p*
| HR (95% CI) |
p*
| HR (95% CI) |
p*
| HR (95% CI) |
p*
| |
PPP2R2A (B55α)-/low Cyclin D1high | Present vs. Absent | 2.71(1.17-6.28) | .020 | 2.09(.89-4.96) | .091 | 2.63(1.26-5.11) | .010 | 2.18(1.02-4.66) | .043 |
HER2 | Positive vs. Negative | 3.37(1.41-8.08) | .006 | 3.94(1.27-7.55) | .013 | 3.02(1.37-6.68) | .006 | 2.92(1.29-6.55) | .010 |
Size | >2cm vs.<2cm | 5.19(1.95-13.85) | .001 | 3.64(1.39-11.18) | .010 | 6.00(2.48-14.51) | <.001 | 4.47(1.91-2.14) | .001 |
Lymph node status# | LNN vs. Positive | 4.13(1.47-11.58) | .007 | 2.49(0.84-7.34) | .099 | 4.08(1.71-9.72) | .002 | 2.36(.99-5.64) | .052 |
Ki-67 index | >14% vs.≥14% | 1.88(.84-4.19) | .12 | - | - | 2.46(1.25-4.83) | .009 | - | - |