Background
Methods
Study design
Detection of Total-P16M and P16H
Definition of true-P16M and P16H status
Follow-up examination and histopathology
Statistical analysis
Results
Patients’ basic information
Status of P16 methylation and hydroxymethylation | Total-P16M- negative | Total-P16M-positive | Total | ||
---|---|---|---|---|---|
Subgroup | (P16U) | (All) | True-P16M | P16H | |
Case number |
174
|
73
|
50
|
23
|
247
|
Age (yrs, mean±SD) |
55.9±10.3
|
58.1±9.7
|
59.3±9.4
|
55.4±10.1
|
56.6±10.2
|
Sex ratio (male, %) |
48.3
|
38.4
|
36.0
|
43.5
|
45.3
|
Cigarette smoking (yes, %) |
33.3
|
17.8
a
|
22.0
|
8.7
|
28.7
|
Alcohol drinking (yes, %) |
24.1
|
19.2
|
18.0
|
21.7
|
22.7
|
Baseline grade (mild, %) |
69.5
|
58.9
|
58.0
|
60.9
|
59.9
|
Lesion site (tongue, %) |
39.1
|
56.2
b
|
54.0
|
60.9
|
44.1
|
Oral Leukoplakia (%) |
69.1
|
30.9
|
21.7
|
9.2
| (n = 217) |
Oral Lichen Planus (%) |
79.3
|
20.7
|
10.3
|
10.3
| (n = 29) |
Discoid lupus erythematous |
1
|
0
|
0
|
0
| (n = 1) |
A similar effect of true-P16M and P16H on predicting malignant progression of oral epithelial dysplasia
Item | All cases | Total-P16M -negative (P16U) | Total-P16M -positive | Odd ratio (95% CI) in univariate analysis | |||||
---|---|---|---|---|---|---|---|---|---|
n
| Total-P16M-positive rate (%) | Cancer rate (%) |
n
| Cancer cases (%) |
n
| Cancer cases %) | |||
Sex | |||||||||
Male | 112 | 25.0 | 8.9 | 84 | 5 (6.0) | 28 | 5 (17.9) | ||
Female | 135 | 33.3 | 16.3 | 90 | 10 (11.1) | 45 | 12 (26.7) | 2.91 (1.15–7.39) | |
Age (yrs) | |||||||||
< 60 | 153 | 26.8 | 11.8 | 112 | 9 (8.0) | 41 | 9 (22.0) | 3.22 (1.18–8.80) | |
≥ 60 | 94 | 34.0 | 14.9 | 62 | 6 (9.7) | 32 | 8 (25.0) | ||
Cigarette smoking | |||||||||
Yes | 71 | 18.3 | 8.5 | 58 | 4 (6.9) | 13 | 2 (15.4) | ||
No | 176 | 34.1 | 14.8 | 116 | 11 (9.5) | 60 | 15 (25.0) | 3.18 (1.36–7.47) | |
Alcohol drinking | |||||||||
Yes | 56 | 25.0 | 8.9 | 42 | 2 (4.8) | 14 | 3 (21.4) | ||
No | 191 | 30.9 | 14.1 | 132 | 13 (9.8) | 59 | 14(23.7) | 2.85 (1.24–6.53) | |
Lesion grade | |||||||||
Mild | 164 | 26.2 | 11.6 | 121 | 10 (8.3) | 43 | 9 (20.9) | 2.94 (1.10–7.82) | |
Mod. | 83 | 36.1 | 15.7 | 53 | 5 (9.4) | 30 | 8 (26.7) | 3.49 (1.02–11.90) | |
Lesion site | |||||||||
Tongue | 109 | 37.6 |
22.0
a,c
| 68 | 9 (13.2) | 41 | 15 (36.6) | 3.78 (1.47–9.75) | |
Others | 138 | 23.2 |
5.8
| 106 | 6 (5.7) | 32 | 2 (6.3) | ||
Center | |||||||||
A | 112 | 33.0 | 11.6 | 75 | 6 (8.0) | 37 | 7 (18.9) | ||
B | 135 | 26.7 | 14.1 | 99 | 9 (9.1) | 36 | 10 (27.8) | 3.85 (1.41–10.46) | |
Sample storage | |||||||||
Frozen | 87 | 32.2 | 12.6 | 59 | 5 (8.5) | 28 | 6 (21.4) | ||
Paraffin | 160 | 28.1 | 13.1 | 115 | 10 (8.7) | 45 | 11 (24.4) | 3.40 (1.33–8.69) | |
Total | 247 | 29.6 | 13.0 | 174 | 15 (8.6) | 73 | 17 (23.3) | 3.22 (1.51–6.87) b,d |