Background
Materials and methods
Patients and tissue samples
Clinical parameters | Cases | RIG-I expression level |
χ
2
|
P
| |
---|---|---|---|---|---|
High (H-score ≥ 45) | Low (H-score < 45) | ||||
Gender | 0.243 | 0.622 | |||
Male | 53 | 38 | 15 | ||
Female | 30 | 23 | 7 | ||
Age (years) | 0.377 | 0.539 | |||
≥ 60 | 56 | 40 | 16 | ||
< 60 | 27 | 21 | 6 | ||
Tumor size (cm) | 0.683 | 0.409 | |||
≥ 5 | 41 | 31 | 10 | ||
< 5 | 41 | 34 | 7 | ||
Pathological stage | 4.668 |
0.031
| |||
I + II | 17 | 16 | 1 | ||
III + IV | 66 | 45 | 21 | ||
Tumor stage | 0.5901 | 0.442 | |||
T1 + T2 | 12 | 10 | 2 | ||
T3 + T4 | 70 | 51 | 19 | ||
Lymph node metastasis | 0.326 | 0.568 | |||
Yes | 65 | 49 | 16 | ||
No | 19 | 17 | 2 | ||
Distant metastasis | 0.881 | 0.348 | |||
Yes | 10 | 9 | 1 | ||
No | 74 | 57 | 17 | ||
TNM stage | 0.561 | 0.454 | |||
I + II | 33 | 26 | 7 | ||
III + IV | 49 | 35 | 14 |
Antibodies and other reagents
Immunohistochemistry and the evaluation of immunostaining
shRNA
Cell culture and treatment
Real-time polymerase chain reaction
Western blotting analysis
Agilent lncRNA microrray analysis
Statistical analyses
Results
Expression of RIG-I in adjacent normal tissues and gastric cancer tissues
Correlation between RIG-I expression in gastric cancer tissues and clinical parameters as well as patients’ prognoses
Clinical parameters | Uni-variate | Multi-variate | ||
---|---|---|---|---|
HR (95% CI) |
P
| HR (95% CI) |
P
| |
Gender (M/F) | 0.924 (0.528–1.616) | 0.781 | 0.733 (0.399–1.350) | 0.319 |
Age (years) (≥ 60/< 60) | 1.391 (0.736–2.536) | 0.281 | 1.916 (1.010–3.636) |
0.047
|
Tumor size (≥ 5 cm/< 5 cm) | 1.726 (0.999–2.984) | 0.051 | 1.077 (0.575–2.017) | 0.816 |
Pathological stage (III + IV/I + II) | 1.961 (0.883–4.355) | 0.098 | 0.860 (0.358–2.067) | 0.860 |
Tumor stage (T3 + T4/T1 + T2) | 2.564 (0.923–7.127) | 0.071 | 1.705 (0.588–4.941) | 0.326 |
Lymph node metastasis (yes/no) | 2.494 (1.170–5.314) |
0.018
| 1.837 (0.621–5.437) | 0.272 |
Distant metastasis (yes/no) | 2.547 (1.187–5.466) |
0.016
| 2.117 (0.907–4.940) | 0.083 |
TNM stage (III + IV/I + II) | 3.017 (1.620–5.617) |
0.000
| 2.503 (1.047–5.980) |
0.039
|
RIG-I expression (High/low) | 0.400 (0.226–0.708) |
0.023
| 0.404 (0.210–0.777) |
0.007
|