Background
Methods
Origin of faecal samples
Sites
|
Abbreviations
|
Coordinates
| |
---|---|---|---|
(Degree, minute, second)
| |||
Lope |
LP
|
S0°13′21.5″
|
E11°36′ 37.5″
|
Lope-Mikongo |
LPM
|
S0°18′27.2″
|
E11°40′10.3″
|
Tsouba |
TB
|
S1°09′54.2″
|
E14°26′46.8″
|
Makande |
MD
|
S0°40′53.7″
|
E11°55′34.4″
|
Langoue |
LG
|
N0°00′05.8″
|
E12°27′25.9″
|
Parc Ivindo-Langoue |
PIL
|
S0°11′23.0″
|
E12°34′58.4″
|
Parc Ivindo-Iret-Kongou-Djidji |
PIKD
|
N0°30′05.2″
|
E12°48′ 03.4″
|
Makatamangoye 1 |
MAK1
|
S0°08′39.3″
|
E13°36′47.6″
|
Monts De Cristal |
MC
|
N0°40′15.4″
|
E10°24′54.2″
|
Parc Ivindo |
PI
|
N0°23′24.8″
|
E12°41′33.1″
|
Djidji |
DJ
|
N0°10′41.2″
|
E12°43′51.8″
|
Mwagna |
MG
|
N0°38′53.5″
|
E13°52′08.2″
|
Boumango |
BG
|
S1°43′36.0″
|
E14°03′10.0″
|
Malouma |
ML
|
N0°39′01.6″
|
E13°52′17.2″
|
Lope 3 |
LP3
|
S0°19′32.4″
|
E11°37′23.6″
|
Gabonville |
GV
|
N1°46′55.7″
|
E11°56′58.4″
|
Tomassi |
TM
|
N1° 06′37.0″
|
E11°42′42.4″
|
Iyokomilieu |
IYO
|
N0°02′54.1″
|
E13°36′05.6″
|
Boue |
BE
|
S0°11′52. 7″
|
E12°02′01.8″
|
Parc de Loango |
PL
|
S1°59′54.8″
|
E9°27′10.5″
|
Waka |
WK
|
S1°07′57.3″
|
E11°08′30.8″
|
Bakoumba |
BKB
|
S1°45′47.8″
|
E12°57′06.2″
|
Lope-Nord |
LPN
|
N0°18′52.1″
|
E12°34′37.7″
|
Konossaville |
KSV
|
N1°40′23.9″
|
E12°04′ 09.7″
|
Extraction of DNA and PCR
Species identification in mixed infections
Host
|
Percentage (%) of mixed infections
|
Associated species (n)
|
---|---|---|
P. reichenowi + P. gaboni(6) | ||
P. billcollinsi + P. gaboni(1) | ||
Chimpanzees | 20% (8/40) |
P. reichenowi + P. billcollinsi(1) |
Gorillas | 28% (8/29) |
P. adleri + P. praefalciparum(7) |
P. adleri + P. blacklocky(1) |
Phylogenetic analyses
Accession number
|
Isolates
|
Species
|
Host species
|
References
|
---|---|---|---|---|
HM235178
| C1 BBptt238 |
P. reichenowi
| Chimpanzee | Liu et al. [12] |
HM235317
| C2 LBptt176 |
P. gaboni
| Chimpanzee | Liu et al. [12] |
HM234979
| C2 BBptt93 |
P. gaboni
| Chimpanzee | Liu et al. [12] |
HM234980
| C1 BBptt93 |
P. reichenowi
| Chimpanzee | Liu et al. [12] |
HM234976
| C3 BApts1413 |
P. billcollinsi
| Chimpanzee | Liu et al. [12] |
KC203544
| EC4014_SGA500.11 |
P. falciparum
| Human | Sundararaman et al. [25] |
FJ895308
| Isolate B |
P. gaboni
| Chimpanzee | Ollomo et al. [11] |
GU045315
| BQ642 |
P. reichenowi
| Chimpanzee | Prugnolle et al. [2] |
GQ355486
| DRCJ |
P. malariae
| Bonobo | Krief et al. [26] |
FJ409564
| CPZcam91 |
P. ovale
| Chimpanzee | Duval et al. [27] |
KF591814
| MRL49_FD_SGA1k. |
P. vivax
| Human | Liu et al. [14] |
JQ345521
| KN013 |
P. knowlesi
| Human | Neoh Wan Fen et al. [28] |
GU045317
| BQ668 |
P. blacklocki
| Gorilla | Prugnolle et al. [2] |
GU045322
| BQ638 |
P. adleri
| Gorilla | Prugnolle et al. [2] |
HM235386
| G1 DDgor27 |
P. praefalciparum
| Gorilla | Liu et al. [12] |
HM235295
| G3 DSgor24 |
P. blacklocki
| Gorilla | Liu et al. [12] |
HM235203
| G1 DSgor86 |
P. praefalciparum
| Gorilla | Liu et al. [12] |
HM235059
| G2 KKgor2638 |
P. adleri
| Gorilla | Liu et al. [12] |
JF923762
| MO454 |
P. praefalciparum
|
C. nictitans
| Prugnolle et al. [2] |
GU815512
| Louise |
P. billcollinsi
| Chimpanzee | Kaiser et al. [8] |
GQ355478
| UGF |
P. billcollinsi
| Chimpanzee | Krief et al. [26] |
GQ355477
| UGD |
P. billcollinsi
| Chimpanzee | Krief et al. [26] |
AJ251941
| - |
P. reichenowi
| Chimpanzee | Conway et al. [29] |
JX893151
| Clone39C |
P. gaboni
| Chimpanzee | Pacheco et al. [30] |
JX893154
| Clone20A |
P. reichnowi
| Chimpanzee | Pacheco et al. [30] |
Statistical analyses
GeneBank accession numbers published in this study
Results
Plasmodium species infecting great apes in Gabon
Variable
|
P-value
|
Odds ratio [CI
95%
]
|
---|---|---|
Host species | 0.051390 | 0.67 [0.503 to 0. 905] |
Freshness of the faeces | 0.006684 | 2.038[1.458 to 2. 849] |
Pluviometry | 0.581011 | 0.576 [0.429 to 0. 775] |