Background
Methods
Sample and serum information
Amplification of full-length pvmsa180
Recombinant PvMSA180 expression
SDS-PAGE and Western blotting
Protein microarray
Production of mice antibody
Immunofluorescence assays
Sequence diversity and haplotype analysis of pvmsa180 in isolates obtained worldwide
Statistical analysis
Results
Schematic structure of PvMSA180
Expression of recombinant PvMSA180 proteins
Recognition of recombinant PvMSA180 proteins
Humoral immune response to PvMSA180
Domains | No. of patient samples (n) | Normalized MFIb
| 95% CId
| No. of healthy samples (n) | Normalized MFIb
| 95% CId
|
p valuee
| ||||
---|---|---|---|---|---|---|---|---|---|---|---|
Positive | Negative | Total (%)a
| Positive | Negative | Total (%)c
| ||||||
I | 32 | 40 | 72 (44.4) | 1.02 | 33.5–55.9 | 1 | 23 | (22) 95.8 | 0.61 | 79.8–99.3 |
p < 0.0001 |
II | 12 | 60 | 72 (16.7) | 0.79 | 9.8–26.9 | 1 | 23 | (22) 95.8 | 0.64 | 79.8–99.3 |
p < 0.01 |
III | 29 | 43 | 72 (40.3) | 1.02 | 29.7–51.8 | 1 | 23 | (22) 95.8 | 0.70 | 79.8–99.3 |
p < 0.0001 |
IV | 30 | 42 | 72 (41.7) | 0.98 | 30.9–53.2 | 0 | 24 | (24) 100 | 0.64 | 86.2–100 |
p < 0.0001 |