Background
Glial cells have long been viewed as static constituents of the CNS, primarily serving support functions. This view was dramatically changed when Garrison et al. found that spinal cord astrocytes were activated following nerve injury in 1991 [
1]. Accumulating evidence implicates that spinal cord glia are key players in the development and maintenance of pathological pain [
2,
3]. There are three major groups of glial cells: astrocytes, microglia, and oligodendrocytes. Astrocytes represent the largest glial cell population in the CNS. Compared with microglia and oligodendrocytes, astrocytes are more closely associated with neurons and blood vessels. One single astrocyte can enwrap four to eight neuronal somata and make a connection with 300–600 neuronal dendrites [
4], which allow astrocytes to regulate the external chemical environment of neurons during synaptic transmission. Increasing evidence indicates that microglia play a role in the development of chronic pain conditions, while astrocytes are more important for the maintenance of chronic pain [
5]. Notably, several studies showed that bone cancer pain, oxaliplatin- and bortezomib-induced peripheral neuropathy, and collagen-induced arthritis pain models showed marked spinal astrocyte activation with limited microglial activation [
6‐
8]. Although targeting astrocytes is beneficial in treating chronic pain, the inhibition of astrocytes may also attenuate normal astrocyte functions, such as glutamate uptake. Therefore, therapeutic strategies should be directed at more specific astrocyte functions or at specific aspects of reactive astrogliosis, by targeting astrocyte-related molecular mechanisms. However, the molecular mechanisms that regulate specific aspects of reactive astrogliosis such as cytokine production remain elusive.
Store-operated calcium channels (SOCCs) are highly Ca
2+-selective cation channels that can be activated by depletion of endoplasmic reticulum (ER) calcium stores, as first postulated in 1986 [
9]. SOCCs are composed of ER calcium sensors STIM1/2 and pore-forming proteins Orai1/2/3 in most cell types. SOC entry (SOCE) is a major mechanism for triggering Ca
2+ influx in immune cells. Additionally, SOCE is involved in cytokine production in immune cells [
10‐
12]. Previous studies have demonstrated that YM-58483, a potent inhibitor of SOCCs, blocks SOCCs in immune cells and reduces cytokine production from these cells [
13‐
15]. Our previous study showed that systemic administration of YM-58483 produces strong analgesic and anti-inflammatory actions with peripheral mechanisms associated with reduction of pro-inflammatory cytokines [
16,
17]. Interestingly, intrathecal injection of YM-58483 also attenuates pain hypersensitivity [
17]. However, the mechanisms underlying its central action remain to be determined. It has also been shown that STIM1 and Orai1 mediate SOCE in cortical astrocytes [
18]. It is therefore reasonable to hypothesize that SOCE is an important Ca
2+ signal in spinal astrocytes. In the present study, we demonstrate that SOCCs are expressed in mouse spinal cord astrocytes. We also identified STIM1, STIM2, and Orai1 as the players of SOCE. Importantly, SOCE can induce TNF-α and IL-6 production, and STIM2 and Orai1 play an important role in lipopolysaccharide (LPS)-induced cytokine production from spinal astrocytes. These findings reveal that SOCE is an important component of Ca
2+ signaling in spinal astrocytes and regulates cytokine production, suggesting a potential role of SOCCs in central sensitization.
Methods
Animals
All experiments were done in accordance with the guidelines of the National Institutes of Health and the Committee for Research and Ethical Issues of IASP and were approved by the Drexel University Animal Care and Use Committee. Pregnant CD1 mice were purchased from Charles River (Wilmington, MA) and individually housed in standard cages in 12-h light/dark cycle.
Cell culture
Primary cultures of spinal cord astrocytes were prepared from neonatal (P2–P3) mice. Briefly, neonatal mice were decapitated after inducing hypothermia on ice. A laminectomy was performed and the spinal cord was carefully removed. The meninges were removed, and spinal cord strips were then incubated for 30 min at 37 °C in Hank’s balanced salt solution (HBSS) (Invitrogen, Carlsbad, CA) (in mM: 137 NaCl, 5.4 KCl, 0.4 KH
2PO
4, 1 CaCl
2, 0.5 MgCl
2, 0.4 MgSO
4, 4.2 NaHCO
3, 0.3 Na
2HPO
4, and 5.6 glucose) containing papain (15 U/ml; Worthington Biochemical, Lakewood, NJ), rinsed three times with HBSS, and placed in minimum essential media (MEM) (Invitrogen) containing 10 % fetal calf serum (FCS, Invitrogen). The strips were mechanically dissociated by gently triturating with a pipette. The resulting cells were plated in 75 cm
2 flask. Cells were maintained at 37 °C in a humidified atmosphere containing 5 % CO
2. Culture medium was changed to Dulbecco’s modified Eagle’s medium (DMEM, Invitrogen) containing 10 % FCS after 24 h and then allowed to grow for 6–8 days until 70 % confluent. Cells were shaken in an orbital shaker (320 rpm) for 2 to 3 h to detach cells sitting on top of the astrocyte monolayer (mainly microglia and precursor cells) [
19,
20] and then were trypsinized and re-plated to six-well plates for Western blotting analysis, 96-well plates for enzyme-linked immunosorbent assay, or 12-mm coverslips for calcium imaging in MEM culture media.
Real-time PCR analysis of mRNA expression
Real-time PCR was performed according to our previous study [
21]. Total RNA was extracted from astrocytes using TRIzol Reagent (Molecular Research Center, Inc., Cincinnati, OH). The RNA concentration was determined by optical density at 260 nm. Total RNA was reverse transcribed into cDNA for each sample using a Fermentas cDNA synthesis kit (Thermo Scientific, Rockford, IL) following the manufacturer’s instructions. Specific primers for mouse STIM1 (Mm00486423_m1), STIM2 (Mm01223103_m1), Orai1 (Mm00774349_m1), Orai2 (Mm04214089_s1), Orai3 (Mm01612888_m1), and GAPDH were purchased from Applied Biosystems (Foster City, CA). Real-time quantitative PCR was performed in a 7900HT fast real-time PCR System (Applied Biosystems) using the following amplification conditions: 5 min of initial denaturation at 96 °C, then 35 cycles of 96 °C for 30 s, 55 °C for 30 s, and 72 °C for 1.5 min. The threshold cycle for each gene was determined and analyzed using the relative quantitation software (Applied Biosystems). The relative expression of the target genes was calculated using the 2(−Delta Delta C
T, 2
−∆∆CT) method. The mRNA levels of STIM1, STIM2, Orai1, Orai2, and Orai3 were normalized to the housekeeping gene GAPDH.
Western blot analysis
Astrocytes were washed in PBS and lysed in an ice-cold radio immunoprecipitation assay (RIPA) buffer containing 50 mM Tris HCl, 150 mM NaCl, 0.2 mM EDTA, 1 % Triton X-100, 2 % sodium dodecyl sulfate, 1 % deoxycholate, 0.1 mM phenylmethanesulfonyl fluoride, and protease inhibitor cocktails (Thermo Fisher Scientific). The lysed astrocytes were then sonicated at a constant intensity of 2.5 for 10 s, and centrifuged at 12,000 RPM (4 °C) for 5 min. The concentration of total protein was determined using a Pierce bicinchoninic acid protein assay kit (Thermo Fisher Scientific) following the manufacturer’s instructions. Protein samples were heated at 95 °C for 5 min, electrophoresed in 10 % sodium dodecyl sulfate polyacrylamide gel, and transferred onto polyvinylidene fluoride membranes (Millipore, Billerica, MA). The blots were blocked with 5 % skimmed milk in Tris-buffered saline–Tween 0.1 % for 1 h at room temperature and probed with rabbit anti-STIM1 (1:8000, Cell Signaling, Danvers, MA), anti-STIM2 (1:8000, ProSci, Inc., Poway, CA), anti-Orai1 (1:500, ProSci), anti-Orai2 (1:500, ProSci), anti-Orai3 (1:500, ProSci), and anti-GAPDH (1:10,000, Cell Signaling) primary antibodies at 4 °C overnight. The blots were washed and incubated for 1 h at room temperature with the horseradish peroxidase–conjugated secondary antibody (1:10,000, Cell Signaling) and then developed with enhanced chemiluminescence (Millipore). The densitometry of protein bands was quantified using Image J software (NIH).
Calcium imaging
Calcium imaging was performed using fura-2-based microfluorimetry and imaging analysis as we previously described [
21]. Astrocytes were loaded with 4 μM of fura-2AM (Life Technologies, Grand Island, NY) for 30 min at room temperature in HBSS, washed, and further incubated in normal bath solution (Tyrode’s) containing (in mM) 140 NaCl, 5 KCl, 2 CaCl
2, 1 MgCl
2, 10 HEPES, and 5.6 glucose for 20 min. Coverslips were mounted in a small laminar-flow perfusion chamber (Model RC-25, Warner Instruments, Hamden, CT) and continuously perfused at 5 to 7 ml/min with Tyrode’s solution. Images were acquired at 3-s intervals at room temperature (20–22 °C) using an Olympus inverted microscope equipped with a CCD camera (Hamamatsu ORCA-03G, Japan). The fluorescence images were recorded and analyzed using the software MetaFluor 7.7.9 (Molecular Devices). The fluorescence ratio was determined as the fluorescence intensities excited at 340 and 380 nm with background subtraction. Only one recording was made from each coverslip.
Transfection
For transfection of siRNAs, astrocytes were cultured on glass coverslips (for calcium imaging) and plates (for PCR and Western blot analysis) for 24 h and then transfected with STIM1 siRNA (Dharmacon, Lafayette, CO, USA), STIM2 siRNA, or Scramble siRNA (Dharmacon, Lafayette, CO) using X-tremeGENE HP Transfection Reagent (Roche Applied Science, Indianapolis, USA) following the manufacturer’s instructions as described in our previous study [
21]. For transfection of Orai siRNAs, astrocytes were electroporated using a mouse Nucleofector kit according to the manufacturer’s instructions (Lonza Group Ltd, Basel, Switzerland) as described in our previous study [
21]. Briefly, astrocytes were transfected with 12 μg of Orai1 siRNA (Life Technologies, Grand Island, NY), Orai2 siRNA (Dharmacon), Orai3 siRNA (Dharmacon), or Scramble siRNA (Life Technologies for Orai1; Dharmacon for Orai2 and Orai3) per 1 × 10
6 cells. Calcium imaging and Western blot analysis were performed 48–72 h post transfection. For transfection of STIM1-YFP and Orai1-CFP (generous gifts from Dr. Gill, Temple University, PA), astrocytes were electroporated using mouse primary cell Nucleofector kit according to the manufacturer’s instructions (Lonza Group Ltd, Basel, Switzerland). Briefly, astrocytes were transfected with 1.5 μg STIM1-YFP alone or co-transfected with Orai1-CFP (2.5 μg) per 1 × 10
6 cells and were seeded on 15-mm glass coverslips. After 16 h, transfection medium was removed and astrocytes were fed with fresh culture medium.
Live-cell confocal imaging
All fluorescence images were captured 48–72 h post transfection using the Olympus FLUOVIEW FV1000 confocal microscope equipped with a ×60 oil-immersion objective. Time-lapse imaging was performed in STIM1-YFP transfected astrocytes. Images of YFP were then acquired at 20-s intervals using a 515-nm laser line for YFP excitation, and YFP emission through a 535- to 565-nm window. For STIM1-YFP and Orai1-CFP co-transfected astrocytes, images of YFP and CFP were acquired at 2-min intervals using 515- and 458-nm laser lines for excitation. All experiments were performed in Tyrode’s solution containing (mM) 140 NaCl, 5 KCl, 1 MgCl2, 2 CaCl2, 10 HEPES, and 5.6 glucose (pH 7.4) at room temperature. The number of STIM1 puncta was quantified using OLYMPUS FLUOVIEW Ver.3.1b. Regions of interest were randomly selected by drawing 10 by 10 μm square boxes in cell bodies. The same regions were used to count the number of puncta before and after TG or CPA treatment. The puncta were selected as spots of high fluorescence intensity ranging from 0.4 to 2.0 μm in diameter size.
Enzyme-linked immunosorbent assay
Astrocytes were re-plated in 96-well plates (2 × 104 cells per well). For basal cytokine level measurement, astrocytes were treated with 0.1 % DMSO in MEM culture medium. For measuring cytokine production, astrocyte were first incubated in 0 Ca2+ Tyrode’s solution containing (in mM) 140 NaCl, 5 KCl, 1 MgCl2, 10 HEPES, and 5.6 glucose for 2 min, then treated with 1 μM TG or 30 μM CPA for 5 min. The 0 Ca2+ Tyrode’s solution was replaced with MEM culture medium containing TG for 5 or 24 h or CPA for 24 h. For testing the effect of drugs on TG-induced cytokine production, astrocytes were pretreated with different concentrations of drugs for 15 min before application of TG. Astrocytes were centrifuged at 1000 rpm for 5 min, and IL-6 and TNF-α in the supernatant were measured by enzyme-linked immunosorbent assay (ELISA) according to the manufacturer’s instructions (R&D Systems, Minneapolis, MN, USA).
MTT cell viability assay
Astrocytes were cultured and re-plated in 96-well plates (1 × 104 cells/well). Astrocytes were first incubated in 0 Ca2+ Tyrode’s solution containing (in mM) 140 NaCl, 5 KCl, 1 MgCl2, 10 HEPES, and 5.6 glucose for 2 min and then treated with 1 μM TG for 5 min. The 0 Ca2+ Tyrode’s solution was replaced with MEM culture medium containing TG for 5 or 24 h. The cell viability was determined by an assay measuring dehydrogenase activity (Trevigen, Gaithersburg, MD) according to the manufacturer’s instructions. Ten microliters of MTT reagent was added to 100 μl of MEM and incubated for 2 h at 37 °C. Once the purple precipitate was clearly visible intracellularly under the microscope, MTT detergent was added to dissolve the formazan crystals. The absorbance was measured directly from 96-well plates at 570 nm using a microplate reader (Spectramax Plus, Molecular Devices, CA).
Drugs
YM-58483, KN-93, and PD98059 were purchased from Tocris (Minneapolis, MN). Cyclopiazonic acid (CPA), thapsigargin (TG), GdCl3, 2-aminoethyl diphenyl borate (2-APB), and LPS were purchased from Sigma (St. Louis, MO). They were dissolved in Milli-Q water or dimethyl sulfoxide (DMSO) as stock solutions and further diluted to final concentrations in 0.1 % DMSO.
Data analysis
Data are expressed as original traces or as mean ± SEM. Treatment effects were statistically analyzed with a one-way analysis of variance (ANOVA). When ANOVA showed a significant difference, pairwise comparisons between means were performed by the post hoc Bonferroni method. Paired or two-sample Student’s t tests were used when comparisons were restricted to two means. Error probabilities of P < 0.05 were considered statistically significant. The statistical software Origin 8.1 was used to perform all statistical analyses.
Discussion
In the present study, we demonstrated that SOCCs are expressed and functional in spinal astrocytes. We identified STIM1, STIM2, and Orai1 as players responsible for SOCE in spinal astrocytes. Our data revealed that STIMs and Orai1 play a role in TG- and LPS-induced cytokine production. These findings indicate the functional importance of STIM1, STIM2, and Orai1 in spinal astrocytes.
Astrocytes are the predominant cell type responsible for the maintenance of brain homeostasis in the CNS. SOCE is a widely recognized calcium signal in non-excitable cells including rat brain astrocytes [
26]. However, its molecular identity remains controversial. A recent study has shown that STIM1 and Orai1 mediate SOCE in rat cortical astrocytes [
18]. SOCC proteins and SOCE have not been reported in spinal cord astrocytes. In this study, we demonstrated that the members of the SOCC family are differentially expressed in spinal astrocytes. The calcium imaging data showed that depletion of ER calcium stores with TG or CPA-induced robust calcium entry in astrocytes, which is inhibited by SOCC inhibitors 2-APB, GdCl
3, and YM-58483. We further identified the components of SOCCs responsible for SOCE using specific siRNAs that target individual members of the SOCC family. We found that knockdown of STIM1 dramatically decreased TG-induced SOCE. Although STIM1 has been shown to play a crucial role in the store-operated coupling process, the role of STIM2 remained unclear. Some reports showed that STIM2 has no effect or an inhibitory effect on SOCC activation [
27,
28], but our current study, as well as others, have indicated that STIM2 also acts as a Ca
2+ sensor [
21,
23,
29,
30]. Here, we also demonstrated that STIM2 is a significant player of SOCE in spinal astrocytes, though it is not as strong as STIM1. Similar to other cell types [
31,
32], Orai2 and Orai3 are not responsible for the SOCE in spinal astrocytes. Knockdown of Orai1, however, reduced SOCE by 80 %, suggesting that Orai1 might be the only STIM1-activated channel involved in TG-induced SOCE. Thus, we revealed for the first time that STIM1, STIM2, and Orai1 are the molecular components responsible for SOCE in spinal astrocytes.
It is well documented that a decrease in ER Ca
2+ results in a profound intracellular redistribution of STIM1 from a uniform ER pattern to spatially discrete areas termed as puncta. This leads to the activation of the Orai1 channel at the plasma membrane, allowing Ca
2+ entry from the extracellular milieu [
33‐
35]. In this study, we also examined STIM1 redistribution and puncta formation with Orai1. Our live-cell confocal images clearly showed that reduction of ER Ca
2+ by TG or CPA-induced robust STIM1 puncta formation. Interestingly, we also observed that TG and CPA induced Orai1 puncta-like formation. Under the resting state, STIM1 formed a few puncta with Orai1. After TG and CPA treatments, STIM1 formed substantial puncta that co-localized with Orai1. Our result is consistent with the previous findings that Orai1 and STIM1 move in a coordinated fashion to form clusters in plasma membrane [
36].
Pro-inflammatory cytokine production is one of the biological markers of astrocyte activation. Numerous studies reported that pro-inflammatory cytokines like TNF-α and IL-6 are involved in pain hypersensitivity [
37,
38]. They are up-regulated in pain conditions [
39,
40], and intrathecal injection of these pro-inflammatory cytokines can directly induce pain responses [
41,
42]. Unlike the well-known critical role of SOCCs in cytokine production in immune cells [
10], the role of SOCCs in cytokine production in astrocytes has not been elucidated. In the present study, we discovered that activation of SOCCs by TG or CPA could induce TNF-α and IL-6 production in spinal astrocytes. Both Gd
3+ and YM-58483 significantly decreased the TNF-α and IL-6 production induced by TG. More importantly, we found that knockdown of STIM1 and Orai1 decreased TNF-α and IL-6 production in spinal astrocytes. Consistent with previous studies, we observed that TG reduced cell viability. However, this reduction was not mediated by SOCE since inhibition of SOCE or knockdown of STIM1 and Orai1 did not affect TG-induced reduction of cell viability.
Since inhibition of Ca
2+-ATPase is not a physiological or pathological approach, we stimulated astrocytes using LPS, a well-defined toll-like receptor (TLR) 2/4 agonist. TLR2/4 receptors are implicated in several pathological conditions such as rheumatoid arthritis [
43,
44]. LPS induced a large amount of TNF-α and IL-6 production which was blocked by SOC inhibitors and knockdown of STIM2 and Orai1 but not by knockdown of STIM1. These findings suggest that STIM2 and Orai1 contribute to LPS-induced cytokine production. LPS has been shown to induce Ca
2+ release from Ca
2+ stores and elicit Ca
2+ oscillations in murine macrophages [
45,
46]. Previous studies have demonstrated that STIM2 can activate Ca
2+ oscillations upon smaller decreases in ER Ca
2+ by low-level agonist concentrations [
30,
47]. We used a very low concentration (2 ng/ml) of LPS, which may only activate STIM2. This might explain why a decrease in STIM2 can reduce LPS-induced cytokine production. Another possibility is that STIM2 constitutively interacts with Orai1 to regulate basal Ca
2+ concentration, which enhances transcriptional activity [
48,
49]. However, the exact role of STIM2 and Orai1 in LPS-induced production of pro-inflammatory cytokines and the cross-talk between the STIM2/Orai1 and TLR signaling remain to be explored.
Abbreviations
2-ABP, 2-aminoethyldiphenyl borate; CNS, central nervous system; CPA, cyclopiazonic acid; DMEM, Dulbecco’s modified Eagle’s; ELISA, enzyme-linked immunosorbent assay; ER, endoplasmic reticulum; FCS, fetal calf serum; GFAP, glial fibrillary acidic protein; HBSS, Hank's Balanced Salt Solution; IL-6, Interleukin-6; LPS, lipopolysaccharide; MEM, medium minimum essential media; siRNA, small inhibitory RNA; SOCCs, store-operated calcium channels; SOCE, store-operated Ca2+ entry; STIM, stromal cell-interaction molecule; TG, thapsigargin; TLR, toll-like receptor; TNF-α, tumor necrosis factor-α; YM-58483, N-[4-[3,5-Bis(trifluoromethyl)-1H-pyrazol-1-yl]phenyl]-4-methyl-1,2,3-thiadiazole-5-carboxamide
Acknowledgements
The authors thank Dr. Donald Gill for providing the STIM1-YFP and ORAI1-CFP constructs.