Background
Methods
Sample and clinical data collection
RT-PCR preparation
Risk assignment
Class call concordance determination
Results
RNA input quantity and tumor content
Repeatability- assay robustness
Reproducibility- inter- and intra-assay reliability, inter-operator precision
Avg Class Call for Replicates | |||
---|---|---|---|
Sample | 1&2 | 3&4 | Concordant |
1 | Class 1 | Class 1 | Yes |
2 | Class 1 | Class 1 | Yes |
3 | Class 2A | Class 2A | Yes |
4 | Class 1 | Class 1 | Yes |
5 | Class 1 | Class 1 | Yes |
6 | Class 1 | Class 1 | Yes |
7 | Class 1 | Class 1 | Yes |
8 | Class 2A | Class 2A | Yes |
9 | Class 1 | Class 1 | Yes |
10 | Class 1 | Class 1 | Yes |
11 | Class 1 | Class 1 | Yes |
12 | Class 1 | Class 1 | Yes |
13 | Class 1 | Class 1 | Yes |
14 | Class 1 | Class 1 | Yes |
15 | Class 2A | Class 1 | No* |
16 | Class 1 | Class 1 | Yes |
17 | Class 1 | Class 1 | Yes |
18 | Class 1 | Class 1 | Yes |
19 | Class 2A | Class 2A | Yes |
20 | Class 1 | Class 1 | Yes |
21 | Class 2A | Class 2A | Yes |
22 | Class 1 | Class 1 | Yes |
23 | Class2B | Class2B | Yes |
24 | Class 2A | Class2B | No* |
25 | Class 2A | Class 2A | Yes |
26 | Class2B | Class2B | Yes |
27 | Class 1 | Class 1 | Yes |
28 | Class 2A | Class 2A | Yes |
29 | Class2B | Class2B | Yes |
30 | Class2B | Class 2A | No** |
Avg Class Call for Replicates | |||
---|---|---|---|
Sample | 1&2 | 3&4 | Concordant |
1 | Class 1 | Class 1 | Yes |
2 | Class 1 | Class 1 | Yes |
3 | Class 1 | Class 1 | Yes |
4 | Class 1 | Class 1 | Yes |
5 | Class 2A | Class 2A | Yes |
6 | Class 1 | Class 1 | Yes |
7 | Class 1 | Class 1 | Yes |
8 | Class 2A | Class 2A | Yes |
9 | Class 1 | Class 2A | No* |
10 | Class 1 | Class 1 | Yes |
11 | Class 1 | Class 1 | Yes |
12 | Class 1 | Class 1 | Yes |
13 | Class 1 | Class 1 | Yes |
14 | Class 1 | Class 1 | Yes |
15 | Class 1 | Class 1 | Yes |
16 | Class 2A | Class 2A | Yes |
17 | Class 1 | Class 1 | Yes |
18 | Class 1 | Class 1 | Yes |
19 | Class 2A | Class 2A | Yes |
20 | Class 2A | Class 2A | Yes |
21 | Class 1 | Class 1 | Yes |
22 | Class 1 | Class 1 | Yes |
23 | Class 2A | Class 2A | Yes |
24 | Class 1 | Class 1 | Yes |
25 | Class 1 | Class 1 | Yes |
26 | Class 1 | Class 1 | Yes |
27 | Class 1 | Class 1 | Yes |
28 | Class 2A | Class 2A | Yes |
29 | Class 1 | Class 1 | Yes |
30 | Class 1 | Class 1 | Yes |
31 | Class 1 | Class 1 | Yes |
32 | Class 1 | Class 1 | Yes |
33 | Class 1 | Class 1 | Yes |
34 | Class 1 | Class 1 | Yes |
35 | Class 1 | Class 1 | Yes |
36 | Class 1 | Class 1 | Yes |
37 | Class 2A | Class 2A | Yes |
38 | Class 1 | Class 1 | Yes |
39 | Class2B | Class2B | Yes |
40 | Class2B | Class2B | Yes |
41 | Class 2A | Class 2A | Yes |
42 | Class 2A | Class 2A | Yes |
43 | Class2B | Class2B | Yes |
44 | Class 1 | Class 1 | Yes |
45 | Class 2A | Class 2A | Yes |
46 | Class2B | Class2B | Yes |
Specimen and reagent stability
Additional Characteristics | Sample | Overall Class Call Concordance | Acceptable |
---|---|---|---|
Longevity, RNA Stability | n = 23 | 96% (22/23) | ≥ 90% |
cDNA stability | n = 15 RT hold | 100% (15/15) | ≥ 90% |
Lot to Lot Reagents Reproducibility | n = 10 Extraction | 90% (9/10) | ≥ 90% |
n = 11 RT Kit & RNase | 91% (10/11) | ≥ 90% | |
n = 5 Pooled Assay & Pre-amplification MM | 100% (5/5) | ≥ 90% | |
n = 8 OA & OA MM | 100% (8/8) | ≥ 90% |