Increased cerebrospinal fluid soluble TREM2 concentration in Alzheimer’s disease
verfasst von:
Amanda Heslegrave, Wendy Heywood, Ross Paterson, Nadia Magdalinou, Johan Svensson, Per Johansson, Annika Öhrfelt, Kaj Blennow, John Hardy, Jonathan Schott, Kevin Mills, Henrik Zetterberg
The discovery that heterozygous missense mutations in the gene encoding triggering receptor expressed on myeloid cells 2 (TREM2) are risk factors for Alzheimer’s disease (AD), with only the apolipoprotein E (APOE) ε4 gene allele conferring a higher risk, has led to increased interest in immune biology in the brain. TREM2 is expressed on microglia, the resident immune cells of the brain and has been linked to phagocytotic clearance of amyloid β (Aβ) plaques. Soluble TREM2 (sTREM2) has previously been measured in cerebrospinal fluid (CSF) by ELISA but in our hands commercial kits have proved unreliable, suggesting that other methods may be required. We developed a mass spectrometry method using selected reaction monitoring for the presence of a TREM2 peptide, which can be used to quantify levels of sTREM2 in CSF.
Findings
We examined CSF samples from memory clinics in Sweden and the UK. For all samples the following were available: clinical diagnosis, age, sex, and measurements of the CSF AD biomarkers Aβ42, T-tau and P-tau181. AD patients (n = 37) all met biomarker (IWG2) criteria for AD. Control individuals (n = 22) were cognitively normal without evidence for AD in CSF. We found significantly higher sTREM2 concentration in AD compared to control CSF. There were significant correlations between CSF sTREM2 and T-tau as well as P-tau181. CSF sTREM2 increase in AD was replicated in a second, independent cohort consisting of 24 AD patients and 16 healthy volunteers.
Conclusion
CSF concentrations of sTREM2 are higher in AD than in controls, and correlate with markers of neurodegeneration. CSF sTREM2 may be used to quantify glial activation in AD.
The online version of this article (doi:10.1186/s13024-016-0071-x) contains supplementary material, which is available to authorized users.
Amanda Heslegrave and Wendy Heywood contributed equally to this work.
Competing interests
The authors declare that they have no competing interests.
Authors’ contributions
AJH drafted the manuscript, performed assays and analysed data, WH designed experiments, performed assays and analysed data, RP, NM, JS and PJ provided samples and clinical data, AO and KB performed biochemical assays and analysed data, JH conceived the study, JS provided samples and clinical data and advised on study design, KM designed experiments and analysed data, HZ advised on study design, drafted manuscript, provided samples and clinical data and analysed biochemical data. All authors edited and reviewed the manuscript.
Introduction
Homozygous mutations in the triggering receptor expressed on myeloid cells 2 (TREM2) are known to cause polycystic lipomembranous osteodysplasia with sclerosing leukoencephalopathy, also known as Nasu-Hakola disease (NHD) [1, 2]. The disease is initially characterised by ankle swellings and frequent bone fractures, however as the disease progresses a neurological syndrome develops that resembles the behavioural variant of frontotemporal dementia (bFTD) [3]. Recently, dementia-like symptoms without bone involvement have been reported in patients with homozygous missense mutations in the TREM2 gene [4]. Further to this, heterozygous mutations in the TREM2 gene have been identified as risk factors for Alzheimer’s disease (AD), Parkinson’s disease (PD), amyotrophic lateral sclerosis (ALS) and FTD [5‐9], although a recent study utilizing detailed clinical phenotyping and TREM2 sequencing found that the most disease-associated mutation (R47H) is a risk factor only for AD and not other neurodegenerative diseases [10]. However, these findings still suggest a crucial role for TREM2 in maintaining homeostasis and modulating inflammation in the brain.
TREM2 is a receptor glycoprotein of 230 amino acids which belongs to the immunoglobulin superfamily. In the brain, TREM2 is expressed exclusively by myeloid cells which include microglia, the brain’s resident immune cells [11]. In vitro, TREM2 promotes phagocytosis, suppresses toll like receptor-induced inflammatory cytokine production and enhances anti-inflammatory cytokine transcription [12, 13].
Anzeige
Given the role TREM2 plays in immune function and the increased risk of disease that variants cause, we set out to investigate TREM2 levels in the CSF of well characterised AD cases and compare them to controls using a novel selected reaction monitoring (SRM) technique.
Methods
Subjects
The study included 37 AD patients who fulfilled the revised proposed International Working Group (IWG2) criteria [14] in which, for research purposes, a clinical diagnosis of AD should be supported by a typical AD biomarker profile of concomitant tau and Aβ pathology. Neurodegeneration and neurofibrillary tangle pathology are reflected in the CSF by increased total tau (T-tau) and phospho-tau (P-tau) concentrations, respectively [15‐18] whereas cerebral Aβ plaque pathology is reflected by reduced CSF levels of the 42 amino acid-long, aggregation-prone Aβ protein (Aβ42) [19, 20]. To determine AD pathology, we used the cut-offs for CSF T-tau, P-tau and Aβ42 established by Duits et al., i.e., a CSF total tau/Αβ42 ratio of more than 0.52, and an Aβ42 concentration of less than 550 pg/ml [21]. Further, 22 control individuals who were cognitively normal and had a negative CSF AD biomarker profile were included. Controls from Sweden were all cognitively normal volunteers (n = 16). The remaining controls from the UK cohort were patients who sought treatment for non-neurodegenerative disorders including depression. One of the AD patients was considered an outlier due to excessively high T-tau and P-tau measurements (T-tau 5894 pg/ml, P-tau 363 pg/ml) and was removed from the analysis. This did not affect the results. In the same round of experiments as for the original AD-control analyses, 6 patients with other neurodegenerative diseases were analysed (2 patients with FTD, 2 patients with semantic dementia, 1 patient with corticobasal syndrome (CBS) and 1 patient with dementia with Lewy bodies (DLB). These were diagnosed according to standard clinical criteria as previously described [22, 23]. Twenty-four AD patients and 16 healthy volunteers, diagnosed according to the same criteria as for the discovery cohort, were used to test for replication of the findings. Neither patients nor controls were on cortisol treatment, but there was no information on the potential use of non-steroidal anti-inflammatory drugs.
The study was approved by the regional ethics committees at UCL and the University of Gothenburg.
Experimental protocols
CSF was collected prospectively in polypropylene tubes according to standard operating procedures, in the morning, at the two sites, then aliquoted into polypropylene tubes and frozen within 2 hours of being collected. After being stored at -80 °C CSF was thawed, then 100ul was pipetted off, freeze dried, and subjected to in-solution digestion. Twenty μl of digest buffer was added (100 mM Tris, pH 7.8 containing 6 M urea, 2 M thiourea and 2 % amidosulfobetaine-14) plus yeast enolase as an internal standard. Protein disulphide bridges were reduced by the addition of 1.5 μL of 100 mM Tris-HCl pH 7.8 containing 5 M dithiothreitol and free thiol groups were carboamidomethylated by incubation with 3 μL of 100 mM Tris-HCl pH 7.8 containing 5 M iodoacetamide. The solution was diluted to a final volume of 200 μl, vortexed and 1 μg of sequence grade trypsin was added. Samples were then incubated for 12-16 hr in a water bath at 37 °C.
Anzeige
In order to design a single reaction monitoring (SRM) assay for TREM2, a unique 13 amino acid-long TREM2 peptide was synthesized. This peptide was chosen so that it was not subject to post-translational modifications such as phosphorylation or glycosylation and not affected by known genetic polymorphisms or mutations (Fig. 1a). The assay was established on a Waters Acquity UPLC coupled to a Xevo TQ-S triple quadrupole mass spectrometer, as previously described [24]. The instrument was operated in positive ion mode. The capillary voltage was maintained at 3.7 kV, with source temperature held constant at 150 °C and nitrogen used as the nebulising gas at a flow rate of 30 L/h. For the TREM2 peptide, precursor ion masses were determined in scan mode with product ion masses determined following collision-induced dissociation with argon. Two transitions were selected for confirmatory purposes; clean transitions without interfering peaks were used. Custom synthesised peptides (Genscript, USA) were used to optimise the peptide detection and to determine the retention time and identify unequivocally the correct peaks/s in CSF. A single 10ul volume of each CSF digest was injected onto a Waters CORTECS UPLC C18 + Column, 90 Å, 3 mm × 100 mm column attached to a C18+ VanGuard pre-column. UPLC and MS tune conditions were performed as described previously [25]. Dynamic Multiple Reaction Monitoring was performed over a 10 minute gradient with a minimum of 0.01 sec dwell time for quantitative transitions and a minimum of 12 data points per peak on Waters Xevo TQ-S MS.
Fig. 1
Increased CSF sTREM2 concentration in AD. a shows a cartoon of the TREM2 peptide indicating in red the peptide sequence and location. b SRM-based analysis of sTREM2 levels in AD cases and controls shows sTREM2 present in all samples (n = 59) but a significant increase (p = 0.0457) is observed in AD cases (n = 37). c There is a significant correlation between CSF T-tau and sTREM2 (r = 0.3863 p = 0.0023). This is also seen (d) when comparing P-tau and sTREM2 (r = 0.5331 p = < 0.0001). However, there is no significant correlation between sTREM2 levels and Aβ42 (r = -0.2097 p = 0.1280) (e). Levels of YKL-40 correlate positively with sTREM2 levels (f) (r = 0.3271 p = 0.0204). g In a second cohort we replicate the finding that CSF sTREM2 levels are increased in AD cases (p = 0.0312)
×
QC runs of pooled CSF digests were run in triplicate at the start of the run and then every 10 injections. A CV within 10 % for each QC was considered acceptable. CSF was spiked with sTREM2 peptides to create a calibration curve with average concentrations of biomarker levels and analysed for intra- and inter-batch variations that were below 10 %. The curve was linear up to 40 pmols.
CSF T-tau, P-tau and Aβ42 were analysed using INNOTEST enzyme-linked immunosorbent assays (ELISAs) (Fujirebio Europe N.V., Gent, Belgium). Plasma C-reactive protein (CRP) was measured using a commercial immunoturbidimetry assay on a Cobas 6000 instrument according to the manufacturer’s instructions (Roche Diagnostics, Penzberg, Germany). CSF YKL-40 and CCL2 were measured with commercially available enzyme-linked immunosorbent assays (R&D Systems, Minneapolis, Minnesota, USA) according to the manufacturer’s instructions.
Data and statistical analysis
Mass spectrometry data were analysed using Mass Lynx version 4.1 and GraphPad Prism. D’Agostino-Pearson test for normality was performed on all data. As most of the parameters displayed skewed distributions, non-parametric tests were used for all comparisons (Mann Whitney U) and correlations (Spearman’s rank). Gender differences were assessed by χ2 test. Threshold for significance was set at p < 0.05.
Results
There were no significant differences in gender, age or plasma CRP breakdown between AD and control groups (Table 1). Despite there being a trend towards higher plasma CRP levels in controls, there was no correlation between plasma CRP levels and CSF sTREM2 levels in AD patients or controls (r = -0.004833 p = 0.9764).
Table 1
Characteristics of AD patients and controls
Subject details
Controls (n = 22)
AD patients (n = 37)
p value
Gender (F/M), no (%)
10(45)/12(55)
19(53)/18(47)
0.6
Age, years (mean ± SD)
69.2 ± 8.0
70.51 ± 7.5
0.54
MMSE score (median IQR)
29.00(26.50-29.00)
22.00(18.00-25.00
<0.0001
APOE ε4 positive (%)
33 %
67 %
0.0002
Plasma CRP (mg/L), median (IQR)
2.095 (0.8425 – 4.7535) n = 16
1.020 (0.6500 – 1.295) n = 25
0.0543
CSF Biomarkers
Aβ1-42 (pg/ml), median (IQR)
978.5(821.8-1045)
367.0(301.0-433.0)
<0.0001
T-tau (pg/ml), median (IQR)
239.5(196.3-279.0)
596.0(455.0-858.5)
<0.0001
P-tau181 (pg/ml), median (IQR)
45.0(36.8-58.3)
95.0(75.0-111.5)
<0.0001
T-tau/Aβ1-42 ratio, median (IQR)
0.25(0.19-0.33)
1.56(1.24-2.55)
<0.0001
sTREM2 (pg/ml), median (IQR)
195.6(131.0-240.7)
231.2(172.5-305.4)
0.0457
Data expressed as mean ± SD or median (IQR) as appropriate. Probability values (p) denote differences between control and AD. A χ2 test was used for gender and APOE genotype comparisons. CSF biomarkers and sTREM2 were evaluated using the Mann-Whitney U test
As expected, the AD subjects had significantly lower MMSE scores and Aβ42 levels, and significantly higher concentrations of T-tau, P-tau and T-tau/Aβ42 ratios compared with controls (Table 1). CSF sTREM2 concentrations were significantly higher in AD patients compared with controls (Table 1, Fig. 1b, p = 0.0457). Analysing the AD and control samples separately, P-tau was positively correlated with sTREM2 in the AD subjects (p = 0.0002) but not in controls. There were no significant correlations between sTREM2 and either T-tau or Aβ42; there was also no correlation between MMSE score and sTREM2 levels in either AD patients or controls. However, when analyzing AD and control individuals together, there was a positive correlation of sTREM2 levels with both T-tau (Fig. 1c, r = 0.3863 p = 0.0023) P-tau (Fig. 1d, r = 0.5331 p = < 0.0001) but not with Aβ42 (Fig. 1e). Cardiovascular risk factors such as stroke, hypertension and smoking were not associated with CSF sTREM2 concentration (p > 0.05 for all comparisons). In a subset (52 subjects), CSF sTREM2 correlated positively with YKL-40 (r =0.3271, p = 0.0204, Fig. 1f) and a similar trend was seen for CSF CCL2, although not significant (r = 0.28, p = 0.07). CSF sTREM2 concentrations were similar in APOE ε4-positive and APOE ε4-negative AD patients (p = 0.5917), as well as in APOE ε4-positive and APOE ε4-negative control individuals (p = 0.2735). In the same round of analyses, CSF sTREM2 was measured in 6 patients with other neurodegenerative diseases (2 patients with FTD, 2 patients with semantic dementia, 1 patient with CBS and 1 patient with DLB). The one CBS case was the only patient with a sTREM2 concentration above 200 pg/mL. The other patients all had control-like concentrations (Additional file 1: Table S1). We replicated the results in an independent study of 24 patients with AD and 16 healthy controls, again showing elevated CSF sTREM2 in AD (Fig. 1 g, p = 0.0312 and Additional file 2: Table S2).
Discussion
Since the publication of papers attributing a higher risk of AD in those carrying variants in the TREM2 gene [5, 26], an enormous amount of work has focused on this gene and its protein product. TREM2 variants have also been implicated in the risk for other neurodegenerative diseases such as PD [6, 7], FTD [7, 8] and ALS [9]. These findings implicate TREM2 in the pathogenesis of AD and likely other neurodegenerative diseases. Here, we show that CSF concentrations of sTREM2 are increased in AD and correlate with markers of neurodegeneration and glial activation, but not amyloid deposition.
In their recent paper, Kleinberger et al. [27] used an in-house ELISA to measure levels of sTREM2 in patients with AD and FTD, as well as in patients with homozygous TREM2 mutations that cause or are predicted to cause loss of function of TREM2 (p.T66M and p.Q33X). Whilst it was impossible to measure sTREM2 in the CSF or serum of the homozygous mutation patients, they did find reduction in CSF sTREM2 in the AD patients [27]. We found these results surprising for a number of reasons. First, TREM2 variants associated with AD and reduced TREM2 secretion are rare and should not influence CSF levels in a downward manner in sporadic AD. Second, a study on a number of different mouse models of AD, which measured mRNA expression in different parts of the brain, showed expression of the Trem2 gene to be increased in cortex, cerebellum, and hippocampus in all models by 18 months of age [28]. Finally, as inflammation has been linked to AD in many studies (for review see [29]), one would expect that CSF markers of neuroinflammation – and so perhaps CSF sTREM2 concentration – would be increased rather than decreased in AD. This is supported by the finding that CSF concentrations of sTREM2 in patients with multiple sclerosis (MS) and other inflammatory diseases were found to be higher than concentrations measured in CSF from non-inflammatory neurologic disease patients [30], corroborating that the marker reflects neuroinflammatory processes in the CNS. Prior to using the method we developed for this study, we attempted to use a commercial ELISA to measure sTREM2 but were unable to validate it.
Anzeige
To explore the relationship between CSF sTREM2 and AD in more detail, we aimed to combine detailed phenotyping of patients and controls with highly accurate measurement of sTREM2. For the former we included conventional CSF biomarkers to ensure as far as possible that individuals with AD had AD pathology; and conversely to ensure a pure control group who did not have evidence of asymptomatic AD pathology. For the latter, we used a very sensitive recently developed SRM assay [24] and adapted it to measure sTREM2. Using these methods, we found a significant increase in sTREM2 concentrations in the CSF of AD subjects and, additionally, we showed that sTREM2 concentrations correlated positively with T-tau and P-tau concentrations in the whole data set, whereas no such correlation was seen for CSF Aβ42. Whilst the correlation was lost for T-tau when considering AD patients separately, the correlation between sTREM2 and P-tau remained. Further, we showed that sTREM2 does not seem to depend on APOE genotype, as there was no difference in CSF sTREM2 concentrations between APOE ε4 carriers and non-carriers. Finally, a positive correlation of CSF sTREM2 with the astroglial marker CSF YKL-40 [31, 32] and a trend for a similar correlation with CSF CCL2, a putative microglial marker [33], support that CSF sTREM2 may reflect glial activation. It may be argued that YKL-40 is expressed in both astrocytes and microglia [31, 32] and that it therefore is hard to dissect the individual contribution of astrocytes and microglia to CSF sTREM2 elevation. However, so far TREM2 expression has only been observed in microglia and not in astrocytes or other cell types of the brain [34, 35]. We therefore find it likely that most CSF sTREM2 represents release from microglia, although we cannot rule out interplay between activated astrocytes and microglia in the release mechanism. In regards to disease specificity, our data prevent us from making a conclusive statement, but it is interesting to note that among the 6 cases with neurodegenerative diseases other than AD, only the one CBS patient (a condition that often displays AD pathology) had a high CSF sTREM2 concentration, whereas the other 5 cases had CSF sTREM2 concentrations in the lower control range. Cases for these other neurodegenerative diseases will be collected so a properly powered study can be conducted in the future.
There are many potential roles for inflammation in the pathogenesis of AD with one possibility being that Aβ deposition occurs independent of inflammatory processes, but that the type and extent of inflammatory response to Aβ deposition within the brain might trigger or influence subsequent neurodegeneration [36]. Our data provide support for this hypothesis: the absence of correlation of CSF sTREM2 and Aβ42 suggests that increased sTREM2 release from microglia is not primarily related to Aβ deposition; whilst the relationship between CSF sTREM2 and tau proteins in particular suggests that an increased inflammatory response is related to neurodegeneration. The mechanism for the release of sTREM2, and also T-tau and P-tau, to the CSF is not known in detail. CSF T-tau concentrations probably reflect the intensity of neuronal damage and degeneration, with higher levels correlating with amount of damaged tissue, rate of progression and mortality [37], i.e. higher amounts of tau are released to CSF with more intense neurodegeneration. For CSF P-tau, the situation is more unclear, since some studies report correlations between high P-tau levels and cortical tangle pathology, while others studies, similar to T-tau, report correlations between high CSF P-tau and higher rate of progression and cognitive decline [37]. These preliminary results do not suggest a mechanism for sTREM2 in the pathogenesis of AD, but do indicate that further studies are required to unravel the role that this protein plays in neuroinflammation and neurodegeneration.
Conclusions
The results of our pilot study suggest that CSF sTREM2 is higher in AD subjects and may be a novel marker of glial activation in AD primarily related to neurodegenerative aspects of the disease and not Aβ pathology. The overlap between cases and controls, however, suggests that the diagnostic usefulness may be limited. Nevertheless, if replicated in larger studies, this may provide important insights into the pathogenic process in AD, and provide a potential biomarker for assessing therapeutic strategies aiming at reducing the inflammatory component of AD and thereby influencing neurodegeneration.
Acknowledgements
We gratefully acknowledge the support of our patients and their families, Alzheimer’s Research UK and Iceland Foods Ltd. This work was supported by the NIHR UCL/UCLH Biomedical Research Centre, the NIHR Queen Square Dementia Biomedical Research Unit, and the Wolfson Foundation. The Dementia Research Centre is an Alzheimer’s Research UK Coordinating Centre. The study was also supported by grants from the Swedish Research Council, the Knut and Alice Wallenberg Foundation, the Torsten Söderberg Foundation, Frimurarestiftelsen and Alzheimerfonden. JMS acknowledges the support of the NIHR Queen Square Dementia BRU, the NIHR UCL/H Biomedical Research Centre, EPSRC (EP/J020990/1), MRC (CSUB19166) and ARUK (ARUK-Network 2012-6-ICE; ARUK-PG2014-1946).
Anzeige
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.
Competing interests
The authors declare that they have no competing interests.
Authors’ contributions
AJH drafted the manuscript, performed assays and analysed data, WH designed experiments, performed assays and analysed data, RP, NM, JS and PJ provided samples and clinical data, AO and KB performed biochemical assays and analysed data, JH conceived the study, JS provided samples and clinical data and advised on study design, KM designed experiments and analysed data, HZ advised on study design, drafted manuscript, provided samples and clinical data and analysed biochemical data. All authors edited and reviewed the manuscript.
Increased cerebrospinal fluid soluble TREM2 concentration in Alzheimer’s disease
verfasst von
Amanda Heslegrave Wendy Heywood Ross Paterson Nadia Magdalinou Johan Svensson Per Johansson Annika Öhrfelt Kaj Blennow John Hardy Jonathan Schott Kevin Mills Henrik Zetterberg
