Skip to main content
Erschienen in: Arthritis Research & Therapy 1/2015

Open Access 01.12.2015 | Research article

Serum progranulin levels are elevated in dermatomyositis patients with acute interstitial lung disease, predicting prognosis

verfasst von: Atsushi Tanaka, Hiroshi Tsukamoto, Hiroki Mitoma, Chikako Kiyohara, Naoyasu Ueda, Masahiro Ayano, Shun-ichiro Ohta, Yasutaka Kimoto, Mitsuteru Akahoshi, Yojiro Arinobu, Hiroaki Niiro, Yoshifumi Tada, Takahiko Horiuchi, Koichi Akashi

Erschienen in: Arthritis Research & Therapy | Ausgabe 1/2015

Abstract

Introduction

Progranulin (PGRN), a pleiotropic growth factor, has emerged as an immunoregulatory molecule. Because the roles of PGRN in dermatomyositis (DM) are still unknown, we investigated whether serum PGRN levels are associated with disease activity and prognosis in DM patients, particularly in those with DM complicated with interstitial lung disease (ILD).

Methods

The serum levels of PGRN were measured by enzyme-linked immunosorbent assay in patients with DM (n =57; acute/subacute interstitial pneumonia (A/SIP): n =17, chronic interstitial pneumonia (CIP): n =24, without ILD: n =16), polymyositis (PM, n =21; including 6 with ILD) and normal healthy controls (NHCs, n =60). We assessed the correlation between the serum PGRN levels and the activity indexes of ILD or prognosis in DM patients with ILD.

Results

Serum PGRN levels were significantly higher in DM patients than in PM patients (P =0.0025) and in NHCs (P <0.0001). In DM patients, the levels were significantly higher in patients with A/SIP than in those with CIP (P <0.0001) or without ILD (P =0.0003). The serum PGRN levels in DM patients with ILD significantly correlated with serum ferritin (r S =0.77, P <0.0001), lactate dehydrogenase (r S =0.54, P =0.0003) and C-reactive protein (r S =0.48, P =0.0015) levels. Moreover, in DM patients with ILD, the cumulative survival rate for 6 months was significantly lower in the group with serum PGRN levels ≥200 ng/ml (67%) than in the group with serum PGRN levels <200 ng/ml (100%) (P =0.0009).

Conclusions

Serum PGRN is associated with disease activity and prognosis of DM with ILD. PGRN may play a role in the pathogenesis of DM and could be a useful biomarker.
Hinweise

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

AT performed the ELISA experiments, the statistical analysis and drafted the manuscript. HT, HM, TH and KA designed the study and helped to draft and revise the manuscript. CK performed the important statistical analysis and helped to revise the manuscript. MAy and SO assisted in conducting the ELISA experiments, contributed to data analysis and helped to draft the manuscript. NU, YK, MAk, YA, HN and YT contributed to the acquisition, analysis and interpretation of clinical data and drafted the manuscript. All authors read and approved the final manuscript.
Abkürzungen
A/SIP
Acute/subacute interstitial pneumonia
ALT
Alanine aminotransferase
ANA
Anti-nuclear antibody
ARDS
Acute respiratory distress syndrome
AST
Aspartate aminotransferase
AZA
Azathioprine
CADM
Clinically amyopathic dermatomyositis
CIP
Chronic interstitial pneumonia
CK
Creatine kinase
CNI
Calcineurin inhibitor
CRP
C-reactive protein
CsA
Cyclosporine A
DM
Dermatomyositis
ELISA
Enzyme-linked immunosorbent assay
γ-GTP
γ-glutamyl transpeptidase
GRN
Granulin
Hb
Hemoglobin
HSCT
Hematopoietic stem cell transplantation
IL
Interleukin
ILD
Interstitial lung disease
IVCY
Intravenous pulse cyclophosphamide
KL-6
Krebs von den Lungen-6
LDH
Lactate dehydrogenase
MTX
Methotrexate
MZB
Mizoribine
NHC
Normal healthy control
NSIP
Nonspecific interstitial pneumonia
PGRN
Progranulin
Plt
Platelet
PM
Polymyositis
PR3
Proteinase 3
PSL
Prednisolone
ROC
Receiver operating characteristic
SLE
Systemic lupus erythematosus
TLR
Toll-like receptor
WBC
White blood cell

Introduction

Dermatomyositis (DM) is a systemic autoimmune and inflammatory disease that involves not only the muscle and skin but also several other organs, such as the lungs, heart and joints [1]. Interstitial lung disease (ILD) is the most common internal organ manifestation, and it affects the prognosis of DM patients. ILD is classified into two subsets: acute/subacute interstitial pneumonia (A/SIP) and chronic interstitial pneumonia (CIP) [2,3]. A/SIP is often complicated with clinically amyopathic DM (CADM) [4,5], which shows the typical skin manifestations of DM but has no or little evidence of clinical myositis [6]. A/SIP complicated with CADM is life-threatening and shows a rapidly progressive pattern, with a 6-month survival rate of 40.8%, irrespective of intensive therapy [5]. Combination therapy with prednisolone (PSL), cyclosporine A (CsA) and intravenous pulse cyclophosphamide (IVCY) is more effective for A/SIP with DM than PSL plus one immunosuppressive agent [7]. Therefore, diagnosis and evaluation of ILD is very important to determine the treatment strategy when DM is diagnosed [8-10]. Although several biomarkers that may reflect inflammatory activity of lung have been utilized, such as ferritin [11-14] and Krebs von den Lungen-6 (KL-6) [15-19], there are no established serum biomarkers for DM-associated ILD.
Progranulin (PGRN) [GenBank:NC_000017] is an extracellular glycoprotein containing seven and one-half repeats of cysteine-rich motifs. PGRN is proteolytically cleaved by extracellular proteases, such as proteinase 3 (PR3) and elastase, into granulin (GRN) [20] that range from 6 to 25 kDa. PGRN is abundantly expressed in rapidly cycling epithelial cells, leukocytes, chondrocytes and neurons [21], and its expression level is at steady state [22]. PGRN plays a critical role in early embryogenesis [22], wound healing [23], maintenance of neuronal survival [24] and tumorigenesis [21]. Particularly, macrophage-derived PGRN is a key regulatory factor in the processes of inflammation and wound healing [23,25]. Recent mouse studies show that mice unable to convert PGRN into GRN due to lack of both elastase and PR3 cannot show inflammation in response to injection of immune complexes [26]. These data indicate that PGRN is synthesized by macrophages and is cleaved into GRNs by elastase in tissues to enhance inflammation. Moreover, PGRN and/or GRN act as a soluble cofactor for Toll-like receptor 9 (TLR9) signaling and enhance it [27]. We previously reported that serum PGRN levels are significantly elevated in systemic lupus erythematosus (SLE) patients in parallel with disease activities and that PGRN may have a role in the pathogenesis of SLE, partly by enhancing the TLR9 signaling and interleukin (IL)-6 production [28]. Although macrophage activation is considered to underlie the pathogenesis of A/SIP with DM [11-14] and TLR9 and IL-6 is associated with DM [29-35], the roles of PGRN in DM are still unknown.
Here, we show that serum PGRN levels are significantly elevated in DM patients, particularly those with A/SIP, and are associated with disease activity and prognosis of DM patients with ILD. PGRN may have a role in the pathogenesis of DM.

Methods

Patients

We performed a cross-sectional study of patients who were treated for DM or polymyositis (PM) at Kyushu University Hospital, Saga University Hospital and Japanese Red Cross Fukuoka Hospital between 2002 and 2013. Fifty-seven Japanese patients with DM and twenty-one patients with PM were enrolled, and sera were obtained from these patients. All of the patients fulfilled the criteria of Bohan and Peter [36] for DM or PM or criteria for CADM [37]. We defined active DM and PM patients who required the initiation or reinforcement of treatment (overall disease activity). We excluded patients with other autoimmune and infectious diseases. Each patient completed a standardized medical history, including drug use, and was given a physical examination. The information obtained from the medical records of the patients included demographic data, such as age, sex, clinical manifestations of DM and laboratory values. Serological profiling of each patient, including white blood cells (WBCs), lymphocytes, hemoglobin (Hb), platelets (Plt), liver enzymes (aspartate aminotransferase (AST), alanine aminotransferase (ALT), γ-glutamyl transpeptidase (γ-GTP)), creatine kinase (CK), lactate dehydrogenase (LDH), ferritin, KL-6, C-reactive protein (CRP), anti-nuclear antibody (ANA) and anti-Jo-1 antibody, was performed using the standard methods. The serum samples from the patients with active DM were acquired before the initiation or reinforcement of treatment, and the samples from the patients with inactive DM were acquired during regular hospital visits. The samples were stored at −20°C. The active DM patients were treated with corticosteroids or immunosuppressive drugs after the completion of these evaluations. The sera obtained from five DM patients with ILD were reevaluated after ILD was ameliorated by treatment. Control sera were obtained from healthy staff members (n =60) at Kyushu University Hospital. This study was approved by the ethics committees of Kyushu University Hospital, Saga University Hospital and Japanese Red Cross Fukuoka Hospital, and the principles of the Declaration of Helsinki were followed throughout the study. Informed consent was obtained from all participants.

Measurement of PGRN

The serum PGRN levels were determined using enzyme-linked immunosorbent assay (ELISA) kits (R&D Systems, Minneapolis, MN, USA) according to the manufacturer’s protocol. Briefly, calibrators, control sera and patients’ sera (stored samples) were diluted and incubated with a mouse monoclonal antibody against PGRN adsorbed onto the microtiter plate wells. After washing, a mouse monoclonal antibody against PGRN conjugated to horseradish peroxidase was added, followed by a second washing step and the addition of tetramethylbenzidine substrate. The intensity of the blue color developed was in proportion to the amount of PGRN bound in the initial step. The reaction was terminated by the addition of 2 N sulfuric acid. The absorbance was measured in a microtiter plate reader (Thermo Fisher Scientific, Waltham, MA, USA) and converted into nanograms per milliliter by plotting the values against the PGRN titer of the calibrators/standards given by the manufacturer. The assay range was 1.56 to 100 ng/ml.

Classification of ILD

ILD was assessed by radiography and high-resolution computed tomography of the chest. ILD with DM was divided into two forms: A/SIP and CIP. A/SIP is defined as a rapidly progressive ILD within 3 months from the onset of symptoms and radiographically diffuse alveolar damage pattern or nonspecific interstitial pneumonias (NSIP). CIP is defined as an asymptomatic, non–rapidly progressive ILD or slowly progressive ILD over the course of 3 months and radiographically confirmed NSIP or bronchiolitis obliterans with organizing pneumonia pattern, according to the guidelines of the International Consensus Statement of idiopathic Pulmonary Fibrosis of the American Thoracic Society and the European Respiratory Society [38]. The designation of A/SIP and CIP was made blindly without knowledge of the laboratory results.

Statistical analysis

The differences between two groups were analyzed using the Mann–Whitney U test. Fisher’s exact test was used for comparison of frequencies. The Steel-Dwass test was used for multiple comparisons among all groups. The relationships between PGRN levels and other continuous variables were analyzed using Spearman’s rank correlation. Receiver operating characteristic (ROC) analyses were performed for a mathematical expression of different serum PGRN levels as cutoff points. The cumulative survival rate was calculated using the Kaplan-Meier test. The log-rank test was also used to compare survival. P-values less than 0.05 were considered significant. All tests were two-tailed. All analyses were performed using JMP statistical software (SAS Institute, Cary, NC, USA).

Results

Serum PGRN levels were markedly elevated in patients with DM

Of the 57 patients with DM enrolled in the present study, 49 were women, and 8 were men (active: n =40, inactive: n =17). These patients ranged in age from 30 to 87 years (median age: 53 years). Of the 21 patients with PM, 16 were women, and 5 were men (active: n =11, inactive: n =10). These patients ranged in age from 15 to 85 years (median age: 53 years). Among the NHCs, 51 were women, and 9 were men. They ranged in age from 20 to 59 years (median age: 32 years). There were significant differences between patients with DM or PM and NHCs in terms of age. However, there was not a significant correlation between serum PGRN levels and age in NHCs.
To investigate the association of PGRN with DM, we first compared serum PGRN levels among 57 patients with DM, 21 patients with PM and 60 NHCs by using ELISA (Figure 1). Serum PGRN levels in NHCs were always within the range of 35 to 70 ng/ml and distributed normally. Serum PGRN levels in patients with PM (median: 60 ng/ml) were significantly higher than in healthy controls (48 ng/ml) (P <0.0001). Strikingly, serum PGRN levels in patients with DM (median: 109 ng/ml) were significantly and markedly higher than in PM patients (P =0.0025) and in healthy controls (P <0.0001).

Serum PGRN levels were elevated in DM patients with A/SIP

Because DM is occasionally complicated with ILD [8-10], we next compared serum PGRN levels among DM patients with A/SIP (n =17), those with CIP (n =24) and those without ILD (n =16) (Figure 2). Serum PGRN levels in DM patients with A/SIP (median: 228 ng/ml) were significantly higher than in DM patients with CIP (75 ng/ml, P <0.0001) or without ILD (79 ng/ml, P =0.0003). There was no significant difference between serum PGRN levels in DM patients with CIP and those without ILD (P =0.8382). However, serum PGRN levels in DM patients with CIP or without ILD were significantly higher than in NHCs (P <0.0001 and P =0.005, respectively; data not shown).

Comparison of clinical manifestations between DM patients with A/SIP and without A/SIP

To evaluate clinical manifestations other than serum PGRN levels, we compared those between DM patients with A/SIP and without A/SIP (Table 1). There were no significant differences among all the subsets in terms of age and sex. The duration between the onset and evaluation in DM patients with A/SIP was significantly shorter than in those without A/SIP. The frequency of muscle weakness was significantly lower in DM patients with A/SIP than in those without it, indicating that the ratio of CADM patients was significantly higher in DM patients with A/SIP than in those without it. WBCs and Hb tended to be lower in DM patients with A/SIP than in those without it. Although CK levels showed no significant difference among all the subsets, AST and ALT levels were significantly higher in DM patients with A/SIP than in those with CIP and without ILD. The levels of LDH, ferritin, KL-6 and CRP, which are used as serologic parameters for disease activity of ILD, showed significant difference between DM patients with A/SIP and those without A/SIP. The frequency of ANA or anti-Jo-1 positivity was not significantly different among all the subsets.
Table 1
Comparison of clinical manifestations between dermatomyositis patients with acute/subacute interstitial pneumonia and without acute/subacute interstitial pneumonia a
Variables
DM with A/SIP
DM with CIP
DM without ILD
Number of patients
17
24
16
Age, yr
54.0 (36 to 65)
54.5 (19 to 78)
36 (15 to 85)
Female, n (%)
14 (82.4)
20 (83.3)
15 (93.8)
Duration, mo
2 (1 to 19)
33 (1 to 148)***
11 (1 to 237)**
Muscle weakness, n (%)
4 (23.5)
15 (62.5)*
15 (94.1)***
WBCs, count/ml
5,200 (2,900 to 12,300)
8,165 (2,900 to 14,990)
7,590 (3,540 to 11,580)
Hb, g/dl
11.6 (7.9 to 13.9)
12.8 (10 to 15.4)
12.5 (9.8 to 15.3)
Plt, ×105/μl
22.2 (14.9 to 34.5)
23.0 (12 to 38.8)
20.6 (6.2 to 37.7)
CK, IU/L
209 (33 to 866)
1,289 (16 to 12,300)
568 (51 to 2,646)
AST, IU/L
74 (31 to 219)
30 (14 to 657)**
44 (12 to 132)*
ALT, IU/L
42 (19 to 111)
20 (11 to 301)*
31 (10 to 150)
γ-GTP, IU/L
43 (16 to 346)
24 (10 to 131)
26 (10 to 165)
LDH, IU/L
392 (225 to 957)
273 (186 to 1,975)*
328 (157 to 489)*
Ferritin, ng/ml
875 (251 to 2,171)
88 (16 to 4,853)***
105 (39 to 400)***
KL-6, U/ml
959 (338 to 2,048)
525 (188 to 1,802)*
224 (125 to 459)***
PGRN, ng/ml
228 (70 to 586)
75 (43 to 221)***
79 (31 to 207)***
CRP, mg/dl
0.86 (0.03 to 2.97)
0.24 (0.03 to 3.66)**
0.08 (0.01 to 0.9)***
ANA >160, n (%)
1 (5.9)
2 (8.3)
5 (31.3)
Anti-Jo-1, n (%)
0 (0)
4 (16.7)
0 (0)
aANA, Anti-nuclear antibody; A/SIP, Acute/subacute interstitial pneumonia; ALT, Alanine aminotransferase; AST, Aspartate aminotransferase; CIP, Chronic interstitial pneumonia; CK, Creatine kinase; CRP, C-reactive protein; DM, Dermatomyositis; γ-GTP, γ-glutamyl transpeptidase; Hb, Hemoglobin; ILD, Interstitial lung disease; KL-6, Krebs von den Lungen-6; LDH, Lactate dehydrogenase; Plt, Platelets; WBC, White blood cell. The values of age, duration, CK, AST, ALT, γ-GTP, LDH, ferritin, KL-6, PGRN and CRP indicate median (range). *P <0.05, **P <0.01, ***P <0.001 compared with DM with A/SIP using the Mann–Whitney U test or Fisher’s exact test.

Serum PGRN levels in DM patients with ILD correlated with disease activities of ILD

We next tested whether serum PGRN levels in DM patients with ILD correlate with conventional serologic parameters for disease activity of ILD (Table 2). Serum PGRN levels showed a significantly positive correlation with serum levels of LDH (r S =0.54, P =0.0003), ferritin (r S =0.77, P <0.0001) and CRP (r S =0.48, P =0.0015). However, there was not a significant correlation between serum levels of PGRN and KL-6 (r S =0.26, P =0.1034). In five DM patients with active ILD that had subsided with treatment, serum PGRN levels decreased in all of them (Figure 3). These results indicate that serum PGRN levels reflect disease activity of ILD in DM patients.
Table 2
Serum progranulin levels in dermatomyositis patients with interstitial lung disease correlate with disease activity of interstitial lung disease a
Variables
r S
P -value
PGRN vs LDH
0.54
0.0003
PGRN vs ferritin
0.77
<0.0001
PGRN vs KL-6
0.26
0.1034
PGRN vs CRP
0.48
0.0015
aCRP, C-reactive protein; KL-6, Krebs von den Lungen-6; LDH, Lactate dehydrogenase; PGRN, Progranulin; r S, Correlation coefficient established employing Spearman’s correlation coefficients. Correlations between the serum PGRN levels and LDH, ferritin, KL-6 and CRP are shown, as measured in 41 DM patients with ILD.

Treatment

The frequency of treatment with PSL alone was fairly low in patients with DM with A/SIP, whereas the frequency of treatment with immunosuppressive agents in addition to PSL was significantly higher in DM patients with A/SIP than in DM patients without ILD (P =0.0004) (Table 3). Immunosuppressive agents used in patients with DM with A/SIP were calcineurin inhibitors (CNIs: CsA or tacrolimus) for nine patients and the combination therapy with CNI plus IVCY for three patients. We performed hematopoietic stem cell transplants (HSCTs) for two refractory DM patients with A/SIP, irrespective of the combination therapy with PSL, CNI plus IVCY, and could rescue them [39].
Table 3
Treatment in each group a
Treatments
DM with A/SIP
DM with CIP
DM without ILD
Number of patients
17
24
16
PSL or other agent alone, n (%)
3 (18)
8 (33)
12 (75)**
PSL
3
7
11
CNI
0
1
0
AZA
0
0
1
PSL + other agents, n (%)
14 (82)
16 (67)
3 (19)**
CNI
9
13
1
MTX
0
0
2
IVCY
0
1
0
CNI + MZB
0
1
0
CNI + IVCY
3
0
0
HSCT
2
1
0
aA/SIP, Acute/subacute interstitial pneumonia; AZA, Azathioprine; CIP, Chronic interstitial pneumonia; CNI, Calcineurin inhibitor (cyclosporine or tacrolimus); DM, Dermatomyositis; HSCT, Hematopoietic stem cell transplant; ILD, Interstitial lung disease; IVCY, Intravenous pulse cyclophosphamide; MTX, Methotrexate; MZB, Mizoribine; PSL, Prednisolone. **P <0.01 compared with DM with A/SIP using Fisher’s exact test.

Serum PGRN levels in DM patients with ILD were associated with prognosis

Because DM with ILD is occasionally life-threatening [4,5,7], in order to define the optimal cutoff point with the highest diagnostic accuracy in terms of survival, we performed ROC curve analysis for distinct serum PGRN levels. The highest area under the curve was calculated for a baseline serum PGRN level of 200 ng/ml. The two subsets were classified by serum PGRN level of 200 ng/ml. The cumulative survival rates for 6 months were 66.7% and 100% in the group with serum PGRN levels ≥200 ng/ml (n =12), including two patients treated with HSCT, and the group with serum PGRN levels <200 ng/ml (n =29) in DM with ILD, respectively (Figure 4). The cumulative survival rate was significantly lower in the group with serum PGRN levels ≥200 ng/ml than in the group with serum PGRN levels <200 ng/ml (P =0.0009). These results suggest that baseline serum PGRN levels predict the prognosis of DM patients with ILD.

Discussion

To our knowledge, this is the first study to show that PGRN levels were significantly elevated in sera of DM patients, in particular those with A/SIP, and these concentrations were associated with the disease activity and prognosis of DM patients with ILD.
Consistent with previous reports [11,12], the elevation of transaminases and hyperferritinemia were observed in DM with A/SIP in the present study. In addition, WBC and Hb tended to be lower in DM patients with A/SIP than in those without it. These facts suggest that macrophages are substantially activated and cause injury to the lungs and the liver in DM patients with A/SIP. Actually, the accumulation of ferritin-producing macrophages has been shown in a CADM-related acute interstitial pneumonia autopsy case [40]. Because PGRN is highly expressed in macrophages [22], elevation of serum PGRN levels in DM with A/SIP also reflects macrophage activation as that of ferritin.
In the present study, other than DM, we also measured concentrations of serum PGRN in patients with PM (n =21), including 6 with ILD (median: 59 ng/ml) and 15 with systemic sclerosis (median: 49 ng/ml) (same levels in NHCs), including 13 with ILD (median: 49 ng/ml). In our data, although sera from PM patients contained significantly elevated PGRN compared with NHCs, DM patients’ sera had much higher levels of PGRN, especially among DM patients who also had A/SIP. This difference may occur because activated macrophages appeared especially in DM patients with A/SIP. Although we could not recruit acute respiratory distress syndrome (ARDS) patients into this study, ARDS could be an additional control to determine whether PGRN is a more general marker of acute respiratory inflammation.
PGRN is reported to be important in the initiation of inflammation by recruiting fibroblasts, macrophages and neutrophils to the site of inflammation [23]. PGRN is also converted to GRN by the elastase that is produced by leukocytes and other cells [41], however, and recent mouse studies have shown that GRN possesses inflammatory functions [25,26,42]. Thus, PGRN may be converted to GRN in the lungs and may be associated with the early phase of pathogenesis in DM with A/SIP. However, it is difficult to investigate the function of GRN in human studies, because we cannot measure GRNs at this moment.
Serum PGRN levels showed a significantly positive correlation with serum levels of LDH (r S =0.54, P =0.0003) (Table 2) and ferritin (r S =0.77, P <0.0001). However, there was no significant correlation between serum levels of PGRN and those of KL-6, another biomarker for ILD. Recent studies suggest serum ferritin levels as a marker for severity of acute progressive ILD in DM and CADM patients [11-14]. The serum ferritin concentrations in CADM patients with A/SIP are already elevated in early stages of the disease, before progression of ILD [11]. LDH and surfactant protein D are released into the blood when pulmonary cells are damaged with inflammation [43]. KL-6 rather reflects the regeneration and proliferation of pneumocytes after the damage of the pulmonary tissue [44]. Thus, PGRN could also be an acute marker for ILD as well as ferritin and LDH.
When we compared clinical manifestations between DM patients with A/SIP and without A/SIP, the frequency of CADM was significantly higher in those with A/SIP (76.5%) than in those with CIP (37.5%) and in those without ILD (5.9%). CADM may be a useful indicator for the occurrence of A/SIP. The levels of CRP showed significant differences between DM patients with A/SIP and those without and correlated with serum PGRN levels (r S =0.48, P =0.0015) (Table 2). We previously reported that serum PGRN levels are elevated in SLE patients and that PGRN stimulated IL-6 production via TLR9 [28]. Upregulated IL-6 production stimulated by PGRN [27,28,45] may be related to elevation of CRP in DM patients with A/SIP. Serum IL-6 levels were significantly higher in dead than alive CADM patients with ILD [33] and were reported to be candidate biomarkers for disease activity in DM [32] and SLE [46]. Because TLR9 is highly expressed in DM [29-31] and SLE [47], upregulated IL-6 production stimulated by PGRN via TLR9 may be a common pathogenesis of DM and SLE. However, the proposed role of PGRN signaling via TLR9 in DM must be directly demonstrated in future studies. Elevated serum PGRN levels have been noted for a number of other pathological conditions [48]. So, PGRN is most likely not a disease-specific marker, but rather is probably a marker of immune activation.
Most of the DM patients with CIP were treated with PSL and immunosuppressive agents. HSCT was performed for one DM patient with CIP and a refractory skin ulcer. In contrast, several patients with A/SIP required combination therapy with PSL, CNI and IVCY (and HSCTs). This distinction might be responsible for cellular phenotypes affecting the pathogenesis of ILD.
The cumulative survival rate was significantly lower in the group with serum PGRN levels ≥200 ng/ml than in the group with serum PGRN levels <200 ng/ml (P =0.0009) (Figure 4). These data indicate that baseline serum PGRN levels can predict survival. The group with serum PGRN levels ≥200 ng/ml included two refractory patients with A/SIP treated with HSCTs, which saved their lives. If we had not been able to rescue them, the survival rate in the group with serum PGRN levels ≥200 ng/ml would have been lower. It is inferred from our findings that the intensive combination therapy with various immunosuppressive agents should be chosen for DM patients with ILD showing high serum PGRN levels, especially ≥200 ng/ml.
We must acknowledge some limitations of this study. The sample size of our study was small. Statistical tests usually require a larger sample size to justify that the effect did not happen by chance alone. Moreover, owing to the study’s cross-sectional design, it is difficult to establish the exact and definite causal relationships, except the association between PGRN and development of ILD with DM, on the basis of the collected data. There were potential confounding effects of medication use on serum PGRN levels in patients with inactive DM with CIP and without ILD. Last, to fully validate PGRN as a predictive biomarker, we need to show that elevated PGRN levels predate clinical respiratory decompensation.

Conclusions

In conclusion, this pilot study demonstrates that the serum PGRN levels were elevated in patients with DM, in particular those with A/SIP, and were correlated with disease activity and prognosis of DM patients with ILD. PGRN could be a useful biomarker for disease activity and a predictor of survival in DM patients with ILD. These findings of the study will provide new insights into the pathogenesis as well as the therapy of DM, and will shed new light on the dysregulation of the immune system in autoimmune diseases. Further studies are required to reveal more precisely the mechanisms of PGRN in human autoimmune diseases.

Acknowledgements

This work was supported in part by grants from the Ministry of Health, Labor and Welfare (H24-Jitsuyoka-Kokusai-004) and from the Ministry of Education, Science, Sports, and Culture of Japan (24591465). We are grateful to the patients and volunteers for their participation in this study and thank all the rheumatologists at the Kyushu University Hospital, Saga University Hospital and Japanese Red Cross Fukuoka Hospital who participated in this study.

Competing interests

The authors declare that they have no competing interests.

Authors’ contributions

AT performed the ELISA experiments, the statistical analysis and drafted the manuscript. HT, HM, TH and KA designed the study and helped to draft and revise the manuscript. CK performed the important statistical analysis and helped to revise the manuscript. MAy and SO assisted in conducting the ELISA experiments, contributed to data analysis and helped to draft the manuscript. NU, YK, MAk, YA, HN and YT contributed to the acquisition, analysis and interpretation of clinical data and drafted the manuscript. All authors read and approved the final manuscript.
Literatur
2.
Zurück zum Zitat Ito M, Kaise S, Suzuki S, Kazuta Y, Sato Y, Miyata M, et al. Clinico-laboratory characteristics of patients with dermatomyositis accompanied by rapidly progressive interstitial lung disease. Clin Rheumatol. 1999;18:462–7.CrossRefPubMed Ito M, Kaise S, Suzuki S, Kazuta Y, Sato Y, Miyata M, et al. Clinico-laboratory characteristics of patients with dermatomyositis accompanied by rapidly progressive interstitial lung disease. Clin Rheumatol. 1999;18:462–7.CrossRefPubMed
3.
Zurück zum Zitat Schnabel A, Reuter M, Biederer J, Richter C, Gross W. Interstitial lung disease in polymyositis and dermatomyositis: clinical course and response to treatment. Semin Arthritis Rheum. 2003;32:273–84.CrossRefPubMed Schnabel A, Reuter M, Biederer J, Richter C, Gross W. Interstitial lung disease in polymyositis and dermatomyositis: clinical course and response to treatment. Semin Arthritis Rheum. 2003;32:273–84.CrossRefPubMed
4.
Zurück zum Zitat Sato S, Hirakata M, Kuwana M, Suwa A, Inada S, Mimori T, et al. Autoantibodies to a 140-kd polypeptide, CADM-140, in Japanese patients with clinically amyopathic dermatomyositis. Arthritis Rheum. 2005;52:1571–6.CrossRefPubMed Sato S, Hirakata M, Kuwana M, Suwa A, Inada S, Mimori T, et al. Autoantibodies to a 140-kd polypeptide, CADM-140, in Japanese patients with clinically amyopathic dermatomyositis. Arthritis Rheum. 2005;52:1571–6.CrossRefPubMed
5.
Zurück zum Zitat Ye S, Chen X, Lu X, Wu MF, Deng Y, Huang WQ, et al. Adult clinically amyopathic dermatomyositis with rapid progressive interstitial lung disease: a retrospective cohort study. Clin Rheumatol. 2007;26:1647–54.CrossRefPubMed Ye S, Chen X, Lu X, Wu MF, Deng Y, Huang WQ, et al. Adult clinically amyopathic dermatomyositis with rapid progressive interstitial lung disease: a retrospective cohort study. Clin Rheumatol. 2007;26:1647–54.CrossRefPubMed
6.
Zurück zum Zitat Euwer RL, Sontheimer RD. Amyopathic dermatomyositis: a review. J Invest Dermatol. 1993;100:124S–7.CrossRefPubMed Euwer RL, Sontheimer RD. Amyopathic dermatomyositis: a review. J Invest Dermatol. 1993;100:124S–7.CrossRefPubMed
7.
Zurück zum Zitat Kameda H, Nagasawa H, Ogawa H, Sekiguchi N, Takei H, Tokuhira M, et al. Combination therapy with corticosteroids, cyclosporin A, and intravenous pulse cyclophosphamide for acute/subacute interstitial pneumonia in patients with dermatomyositis. J Rheumatol. 2005;32:1719–26.PubMed Kameda H, Nagasawa H, Ogawa H, Sekiguchi N, Takei H, Tokuhira M, et al. Combination therapy with corticosteroids, cyclosporin A, and intravenous pulse cyclophosphamide for acute/subacute interstitial pneumonia in patients with dermatomyositis. J Rheumatol. 2005;32:1719–26.PubMed
8.
Zurück zum Zitat Saketkoo LA, Ascherman DP, Cottin V, Christopher-Stine L, Danoff SK, Oddis CV. Interstitial lung disease in idiopathic inflammatory myopathy. Curr Rheumatol Rev. 2010;6:108–19.CrossRefPubMedCentralPubMed Saketkoo LA, Ascherman DP, Cottin V, Christopher-Stine L, Danoff SK, Oddis CV. Interstitial lung disease in idiopathic inflammatory myopathy. Curr Rheumatol Rev. 2010;6:108–19.CrossRefPubMedCentralPubMed
9.
Zurück zum Zitat Connors GR, Christopher-Stine L, Oddis CV, Danoff SK. Interstitial lung disease associated with the idiopathic inflammatory myopathies: what progress has been made in the past 35 years? Chest. 2010;138:1464–74.CrossRefPubMed Connors GR, Christopher-Stine L, Oddis CV, Danoff SK. Interstitial lung disease associated with the idiopathic inflammatory myopathies: what progress has been made in the past 35 years? Chest. 2010;138:1464–74.CrossRefPubMed
10.
Zurück zum Zitat Labirua A, Lundberg IE. Interstitial lung disease and idiopathic inflammatory myopathies: progress and pitfalls. Curr Opin Rheumatol. 2010;22:633–8.CrossRefPubMed Labirua A, Lundberg IE. Interstitial lung disease and idiopathic inflammatory myopathies: progress and pitfalls. Curr Opin Rheumatol. 2010;22:633–8.CrossRefPubMed
11.
Zurück zum Zitat Nakashima R, Imura Y, Kobayashi S, Yukawa N, Yoshifuji H, Nojima T, et al. The RIG-I-like receptor IFIH1/MDA5 is a dermatomyositis-specific autoantigen identified by the anti-CADM-140 antibody. Rheumatology (Oxford). 2010;49:433–40.CrossRef Nakashima R, Imura Y, Kobayashi S, Yukawa N, Yoshifuji H, Nojima T, et al. The RIG-I-like receptor IFIH1/MDA5 is a dermatomyositis-specific autoantigen identified by the anti-CADM-140 antibody. Rheumatology (Oxford). 2010;49:433–40.CrossRef
12.
Zurück zum Zitat Gono T, Kawaguchi Y, Hara M, Masuda I, Katsumata Y, Shinozaki M, et al. Increased ferritin predicts development and severity of acute interstitial lung disease as a complication of dermatomyositis. Rheumatology (Oxford). 2010;49:1354–60.CrossRef Gono T, Kawaguchi Y, Hara M, Masuda I, Katsumata Y, Shinozaki M, et al. Increased ferritin predicts development and severity of acute interstitial lung disease as a complication of dermatomyositis. Rheumatology (Oxford). 2010;49:1354–60.CrossRef
13.
Zurück zum Zitat Gono T, Kawaguchi Y, Satoh H, Kuwana M, Katsumata Y, Takagi K, et al. Clinical manifestation and prognostic factor in anti-melanoma differentiation-associated gene 5 antibody-associated interstitial lung disease as a complication of dermatomyositis. Rheumatology (Oxford). 2010;49:1713–9.CrossRef Gono T, Kawaguchi Y, Satoh H, Kuwana M, Katsumata Y, Takagi K, et al. Clinical manifestation and prognostic factor in anti-melanoma differentiation-associated gene 5 antibody-associated interstitial lung disease as a complication of dermatomyositis. Rheumatology (Oxford). 2010;49:1713–9.CrossRef
14.
Zurück zum Zitat Gono T, Kawaguchi Y, Ozeki E, Ota Y, Satoh T, Kuwana M, et al. Serum ferritin correlates with activity of anti-MDA5 antibody-associated acute interstitial lung disease as a complication of dermatomyositis. Mod Rheumatol. 2011;21:223–7.CrossRefPubMed Gono T, Kawaguchi Y, Ozeki E, Ota Y, Satoh T, Kuwana M, et al. Serum ferritin correlates with activity of anti-MDA5 antibody-associated acute interstitial lung disease as a complication of dermatomyositis. Mod Rheumatol. 2011;21:223–7.CrossRefPubMed
15.
Zurück zum Zitat Bandoh S, Fujita J, Ohtsuki Y, Ueda Y, Hojo S, Tokuda M, et al. Sequential changes of KL-6 in sera of patients with interstitial pneumonia associated with polymyositis/dermatomyositis. Ann Rheum Dis. 2000;59:257–62.CrossRefPubMedCentralPubMed Bandoh S, Fujita J, Ohtsuki Y, Ueda Y, Hojo S, Tokuda M, et al. Sequential changes of KL-6 in sera of patients with interstitial pneumonia associated with polymyositis/dermatomyositis. Ann Rheum Dis. 2000;59:257–62.CrossRefPubMedCentralPubMed
16.
Zurück zum Zitat Kubo M, Ihn H, Yamane K, Kikuchi K, Yazawa N, Soma Y, et al. Serum KL-6 in adult patients with polymyositis and dermatomyositis. Rheumatology (Oxford). 2000;39:632–6.CrossRef Kubo M, Ihn H, Yamane K, Kikuchi K, Yazawa N, Soma Y, et al. Serum KL-6 in adult patients with polymyositis and dermatomyositis. Rheumatology (Oxford). 2000;39:632–6.CrossRef
17.
Zurück zum Zitat Satoh H, Kurishima K, Ishikawa H, Ohtsuka M. Increased levels of KL-6 and subsequent mortality in patients with interstitial lung disease. J Intern Med. 2006;260:429–34.CrossRefPubMed Satoh H, Kurishima K, Ishikawa H, Ohtsuka M. Increased levels of KL-6 and subsequent mortality in patients with interstitial lung disease. J Intern Med. 2006;260:429–34.CrossRefPubMed
18.
Zurück zum Zitat Kumánovics G, Minier T, Radics J, Pálinkás L, Berki T, Czirják L. Comprehensive investigation of novel serum markers of pulmonary fibrosis associated with systemic sclerosis and dermato/polymyositis. Clin Exp Rheumatol. 2008;26:414–20.PubMed Kumánovics G, Minier T, Radics J, Pálinkás L, Berki T, Czirják L. Comprehensive investigation of novel serum markers of pulmonary fibrosis associated with systemic sclerosis and dermato/polymyositis. Clin Exp Rheumatol. 2008;26:414–20.PubMed
19.
Zurück zum Zitat Fathi M, Barbasso Helmers S, Lundberg IE. KL-6: a serological biomarker for interstitial lung disease in patients with polymyositis and dermatomyositis. J Intern Med. 2012;271:589–97.CrossRefPubMed Fathi M, Barbasso Helmers S, Lundberg IE. KL-6: a serological biomarker for interstitial lung disease in patients with polymyositis and dermatomyositis. J Intern Med. 2012;271:589–97.CrossRefPubMed
20.
Zurück zum Zitat He Z, Bateman A. Progranulin (granulin-epithelin precursor, PC-cell-derived growth factor, acrogranin) mediates tissue repair and tumorigenesis. J Mol Med. 2003;81:600–12.CrossRefPubMed He Z, Bateman A. Progranulin (granulin-epithelin precursor, PC-cell-derived growth factor, acrogranin) mediates tissue repair and tumorigenesis. J Mol Med. 2003;81:600–12.CrossRefPubMed
21.
Zurück zum Zitat Bateman A, Bennett HP. The granulin gene family: from cancer to dementia. Bioessays. 2009;31:1245–54.CrossRefPubMed Bateman A, Bennett HP. The granulin gene family: from cancer to dementia. Bioessays. 2009;31:1245–54.CrossRefPubMed
22.
Zurück zum Zitat Daniel R, He Z, Carmichael KP, Halper J, Bateman A. Cellular localization of gene expression for progranulin. J Histochem Cytochem. 2000;48:999–1009.CrossRefPubMed Daniel R, He Z, Carmichael KP, Halper J, Bateman A. Cellular localization of gene expression for progranulin. J Histochem Cytochem. 2000;48:999–1009.CrossRefPubMed
23.
Zurück zum Zitat He Z, Ong CH, Halper J, Bateman A. Progranulin is a mediator of the wound response. Nat Med. 2003;9:225–9.CrossRefPubMed He Z, Ong CH, Halper J, Bateman A. Progranulin is a mediator of the wound response. Nat Med. 2003;9:225–9.CrossRefPubMed
24.
Zurück zum Zitat Van Damme P, Van Hoecke A, Lambrechts D, Vanacker P, Bogaert E, van Swieten J, et al. Progranulin functions as a neurotrophic factor to regulate neurite outgrowth and enhance neuronal survival. J Cell Biol. 2008;181:37–41.CrossRefPubMedCentralPubMed Van Damme P, Van Hoecke A, Lambrechts D, Vanacker P, Bogaert E, van Swieten J, et al. Progranulin functions as a neurotrophic factor to regulate neurite outgrowth and enhance neuronal survival. J Cell Biol. 2008;181:37–41.CrossRefPubMedCentralPubMed
25.
Zurück zum Zitat Yin F, Banerjee R, Thomas B, Zhou P, Qian L, Jia T, et al. Exaggerated inflammation, impaired host defense, and neuropathology in progranulin-deficient mice. J Exp Med. 2010;207:117–28.CrossRefPubMedCentralPubMed Yin F, Banerjee R, Thomas B, Zhou P, Qian L, Jia T, et al. Exaggerated inflammation, impaired host defense, and neuropathology in progranulin-deficient mice. J Exp Med. 2010;207:117–28.CrossRefPubMedCentralPubMed
26.
Zurück zum Zitat Kessenbrock K, Fröhlich L, Sixt M, Lämmermann T, Pfister H, Bateman A, et al. Proteinase 3 and neutrophil elastase enhance inflammation in mice by inactivating antiinflammatory progranulin. J Clin Invest. 2008;118:2438–47.PubMedCentralPubMed Kessenbrock K, Fröhlich L, Sixt M, Lämmermann T, Pfister H, Bateman A, et al. Proteinase 3 and neutrophil elastase enhance inflammation in mice by inactivating antiinflammatory progranulin. J Clin Invest. 2008;118:2438–47.PubMedCentralPubMed
27.
Zurück zum Zitat Park B, Buti L, Lee S, Matsuwaki T, Spooner E, Brinkmann MM, et al. Granulin is a soluble cofactor for Toll-like receptor 9 signaling. Immunity. 2011;34:505–13.CrossRefPubMed Park B, Buti L, Lee S, Matsuwaki T, Spooner E, Brinkmann MM, et al. Granulin is a soluble cofactor for Toll-like receptor 9 signaling. Immunity. 2011;34:505–13.CrossRefPubMed
28.
Zurück zum Zitat Tanaka A, Tsukamoto H, Mitoma H, Kiyohara C, Ueda N, Ayano M, et al. Serum progranulin levels are elevated in patients with systemic lupus erythematosus, reflecting disease activity. Arthritis Res Ther. 2012;14:R244.CrossRefPubMedCentralPubMed Tanaka A, Tsukamoto H, Mitoma H, Kiyohara C, Ueda N, Ayano M, et al. Serum progranulin levels are elevated in patients with systemic lupus erythematosus, reflecting disease activity. Arthritis Res Ther. 2012;14:R244.CrossRefPubMedCentralPubMed
29.
Zurück zum Zitat Kim GT, Cho ML, Park YE, Yoo WH, Kim JH, Oh HJ, et al. Expression of TLR2, TLR4, and TLR9 in dermatomyositis and polymyositis. Clin Rheumatol. 2010;29:273–9.CrossRefPubMedCentralPubMed Kim GT, Cho ML, Park YE, Yoo WH, Kim JH, Oh HJ, et al. Expression of TLR2, TLR4, and TLR9 in dermatomyositis and polymyositis. Clin Rheumatol. 2010;29:273–9.CrossRefPubMedCentralPubMed
30.
Zurück zum Zitat Brunn A, Zornbach K, Hans VH, Haypt WF, Deckert M. Toll-like receptors promote inflammation in idiopathic inflammatory myopathies. J Neuropathol Exp Neurol. 2012;71:855–67.CrossRefPubMed Brunn A, Zornbach K, Hans VH, Haypt WF, Deckert M. Toll-like receptors promote inflammation in idiopathic inflammatory myopathies. J Neuropathol Exp Neurol. 2012;71:855–67.CrossRefPubMed
31.
Zurück zum Zitat Sun WC, Sun YC, Lin H, Yan B, Shi GX. Dysregulation of the type I interferon system in adult-onset clinically amyopathic dermatomyositis has a potential contribution to the development of interstitial lung disease. Br J Dermatol. 2012;167:1236–44.CrossRefPubMed Sun WC, Sun YC, Lin H, Yan B, Shi GX. Dysregulation of the type I interferon system in adult-onset clinically amyopathic dermatomyositis has a potential contribution to the development of interstitial lung disease. Br J Dermatol. 2012;167:1236–44.CrossRefPubMed
32.
Zurück zum Zitat Bilgic H, Ytterberg S, Amin S, McNallan KT, Wilson JC, Koeuth T, et al. Interleukin-6 and type I interferon-regulated genes and chemokines mark disease activity in dermatomyositis. Arthritis Rheum. 2009;60:3436–46.33. Nara M, Komatsuda A, Omokawa A, Togashi M, Okuyama S, Sawada K, et al. Serum interleukin 6 levels as a useful prognostic predictor of clinically amyopathic dermatomyositis with rapidly progressive interstitial lung disease. Mod Rheumatol. 2014;24:633–6.CrossRef Bilgic H, Ytterberg S, Amin S, McNallan KT, Wilson JC, Koeuth T, et al. Interleukin-6 and type I interferon-regulated genes and chemokines mark disease activity in dermatomyositis. Arthritis Rheum. 2009;60:3436–46.33. Nara M, Komatsuda A, Omokawa A, Togashi M, Okuyama S, Sawada K, et al. Serum interleukin 6 levels as a useful prognostic predictor of clinically amyopathic dermatomyositis with rapidly progressive interstitial lung disease. Mod Rheumatol. 2014;24:633–6.CrossRef
33.
Zurück zum Zitat Haddad F, Zaldivar F, Cooper DM, Adams GR. IL-6-induced skeletal muscle atrophy. J Appl Physiol. 2005;98:911–7.CrossRefPubMed Haddad F, Zaldivar F, Cooper DM, Adams GR. IL-6-induced skeletal muscle atrophy. J Appl Physiol. 2005;98:911–7.CrossRefPubMed
34.
Zurück zum Zitat Okiyama N, Sugihara T, Iwakura Y, Yokozeki H, Miyasaka N, Kohsaka H. Therapeutic effects of interleukin-6 blockade in a murine model of polymyositis that does not require interleukin-17A. Arthritis Rheum. 2009;60:2505–12.CrossRefPubMed Okiyama N, Sugihara T, Iwakura Y, Yokozeki H, Miyasaka N, Kohsaka H. Therapeutic effects of interleukin-6 blockade in a murine model of polymyositis that does not require interleukin-17A. Arthritis Rheum. 2009;60:2505–12.CrossRefPubMed
35.
Zurück zum Zitat Bohan A, Peter J. Polymyositis and dermatomyositis (first of two parts). N Engl J Med. 1975;292:344–7.CrossRefPubMed Bohan A, Peter J. Polymyositis and dermatomyositis (first of two parts). N Engl J Med. 1975;292:344–7.CrossRefPubMed
36.
Zurück zum Zitat Sontheimer RD. Would a new name hasten the acceptance of amyopathic dermatomyositis (dermatomyositis siné myositis) as a distinctive subset within the idiopathic inflammatory dermatomyositis spectrum of clinical illness? J Am Acad Dermatol. 2002;46:626–36. A published erratum appears in J Am Acad Dermatol. 2002;46:699.CrossRefPubMed Sontheimer RD. Would a new name hasten the acceptance of amyopathic dermatomyositis (dermatomyositis siné myositis) as a distinctive subset within the idiopathic inflammatory dermatomyositis spectrum of clinical illness? J Am Acad Dermatol. 2002;46:626–36. A published erratum appears in J Am Acad Dermatol. 2002;46:699.CrossRefPubMed
37.
Zurück zum Zitat American Thoracic Society, European Respiratory Society. Idiopathic pulmonary fibrosis: diagnosis and treatment. International Consensus Statement. Am J Respir Crit Care Med. 2000;161:646–64. American Thoracic Society, European Respiratory Society. Idiopathic pulmonary fibrosis: diagnosis and treatment. International Consensus Statement. Am J Respir Crit Care Med. 2000;161:646–64.
38.
Zurück zum Zitat Oryoji K, Himeji D, Nagafuji K, Horiuchi T, Tsukamoto H, Nondo H, et al. Successful treatment of rapidly progressive interstitial pneumonia with autologous peripheral blood stem cell transplantation in a patient with dermatomyositis. Clin Rheumatol. 2005;24:637–40.CrossRefPubMed Oryoji K, Himeji D, Nagafuji K, Horiuchi T, Tsukamoto H, Nondo H, et al. Successful treatment of rapidly progressive interstitial pneumonia with autologous peripheral blood stem cell transplantation in a patient with dermatomyositis. Clin Rheumatol. 2005;24:637–40.CrossRefPubMed
39.
Zurück zum Zitat Gono T, Miyake K, Kawaguchi Y, Kaneko H, Shinozaki M, Yamanaka H. Hyperferritinemia and macrophage activation in a patient with interstitial lung disease with clinically amyopathic DM. Rheumatology (Oxford). 2012;51:1336–8.CrossRef Gono T, Miyake K, Kawaguchi Y, Kaneko H, Shinozaki M, Yamanaka H. Hyperferritinemia and macrophage activation in a patient with interstitial lung disease with clinically amyopathic DM. Rheumatology (Oxford). 2012;51:1336–8.CrossRef
40.
Zurück zum Zitat Kessenbrock K, Dau T, Jenne DE. Tailor-made inflammation: how neutrophil serine proteases modulate the inflammatory response. J Mol Med. 2011;89:23–8.CrossRefPubMedCentralPubMed Kessenbrock K, Dau T, Jenne DE. Tailor-made inflammation: how neutrophil serine proteases modulate the inflammatory response. J Mol Med. 2011;89:23–8.CrossRefPubMedCentralPubMed
41.
Zurück zum Zitat Tang W, Lu Y, Tian QY, Zhang Y, Guo FJ, Liu GY, et al. The growth factor progranulin binds to TNF receptors and is therapeutic against inflammatory arthritis in mice. Science. 2011;332:478–84.CrossRefPubMedCentralPubMed Tang W, Lu Y, Tian QY, Zhang Y, Guo FJ, Liu GY, et al. The growth factor progranulin binds to TNF receptors and is therapeutic against inflammatory arthritis in mice. Science. 2011;332:478–84.CrossRefPubMedCentralPubMed
42.
Zurück zum Zitat Ihn H, Asano Y, Kubo M, Yamane K, Jinnin M, Yazawa N, et al. Clinical significance of serum surfactant protein D (SP-D) in patients with polymyositis/dermatomyositis: correlation with interstitial lung disease. Rheumatology (Oxford). 2002;41:1268–72.CrossRef Ihn H, Asano Y, Kubo M, Yamane K, Jinnin M, Yazawa N, et al. Clinical significance of serum surfactant protein D (SP-D) in patients with polymyositis/dermatomyositis: correlation with interstitial lung disease. Rheumatology (Oxford). 2002;41:1268–72.CrossRef
43.
Zurück zum Zitat Nakajima H, Harigai M, Hara M, Hakoda M, Tokuda H, Sakai F, et al. KL-6 as a novel serum marker for interstitial pneumonia associated with collagen disease. J Rheumatol. 2000;27:1164–70.PubMed Nakajima H, Harigai M, Hara M, Hakoda M, Tokuda H, Sakai F, et al. KL-6 as a novel serum marker for interstitial pneumonia associated with collagen disease. J Rheumatol. 2000;27:1164–70.PubMed
44.
Zurück zum Zitat Matsubara T, Mita A, Minami K, Hosooka T, Kitazawa S, Takahashi K, et al. PGRN is a key adipokine mediating high fat diet-induced insulin resistance and obesity through IL-6 in adipose tissue. Cell Metab. 2012;15:38–50.CrossRefPubMed Matsubara T, Mita A, Minami K, Hosooka T, Kitazawa S, Takahashi K, et al. PGRN is a key adipokine mediating high fat diet-induced insulin resistance and obesity through IL-6 in adipose tissue. Cell Metab. 2012;15:38–50.CrossRefPubMed
46.
Zurück zum Zitat Papadimitraki ED, Choulaki C, Koutala E, Bertsias G, Tsatsanis C, Gergianaki I, et al. Expansion of Toll-like receptor 9-expressing B cells in active systemic lupus erythematosus: implications for the induction and maintenance of the autoimmune process. Arthritis Rheum. 2006;54:3601–11.CrossRefPubMed Papadimitraki ED, Choulaki C, Koutala E, Bertsias G, Tsatsanis C, Gergianaki I, et al. Expansion of Toll-like receptor 9-expressing B cells in active systemic lupus erythematosus: implications for the induction and maintenance of the autoimmune process. Arthritis Rheum. 2006;54:3601–11.CrossRefPubMed
47.
Metadaten
Titel
Serum progranulin levels are elevated in dermatomyositis patients with acute interstitial lung disease, predicting prognosis
verfasst von
Atsushi Tanaka
Hiroshi Tsukamoto
Hiroki Mitoma
Chikako Kiyohara
Naoyasu Ueda
Masahiro Ayano
Shun-ichiro Ohta
Yasutaka Kimoto
Mitsuteru Akahoshi
Yojiro Arinobu
Hiroaki Niiro
Yoshifumi Tada
Takahiko Horiuchi
Koichi Akashi
Publikationsdatum
01.12.2015
Verlag
BioMed Central
Erschienen in
Arthritis Research & Therapy / Ausgabe 1/2015
Elektronische ISSN: 1478-6362
DOI
https://doi.org/10.1186/s13075-015-0547-z

Weitere Artikel der Ausgabe 1/2015

Arthritis Research & Therapy 1/2015 Zur Ausgabe

Leitlinien kompakt für die Innere Medizin

Mit medbee Pocketcards sicher entscheiden.

Seit 2022 gehört die medbee GmbH zum Springer Medizin Verlag

Update Innere Medizin

Bestellen Sie unseren Fach-Newsletter und bleiben Sie gut informiert.