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Erschienen in: Annals of Surgical Oncology 5/2013

01.05.2013 | Translational Research and Biomarkers

Biobanking of Fresh-frozen Human Colon Tissues: Impact of Tissue Ex-vivo Ischemia Times and Storage Periods on RNA Quality

verfasst von: Wei-Guang Bao, MD, Xia Zhang, MD, Jian-Gang Zhang, MD, Wen-Jun Zhou, Tie-Nan Bi, MD, Jiao-Chen Wang, MD, PhD, Wei-Hua Yan, MD, PhD, Aifen Lin, MD, PhD

Erschienen in: Annals of Surgical Oncology | Ausgabe 5/2013

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Abstract

Background

Biobanking plays an important role in translational cancer research. The impact of tissue ex-vivo ischemia time and storage period on RNA integrity is not well documented.

Methods

Fresh-frozen colon tissues were collected in Taizhou Hospital of Zhejiang Province in China since 2004. Fifty-one colon cancer tissues with tumor cell content higher than 70 % and matched normal tissues during four storage periods (less than 15 months, 16–20 months, 21–25 months, and 26–40 months) were chosen to detect RNA quality. Fresh colon cancer tissues from 5 patients were cut into pieces and kept at room temperature or on ice for 0.5, 1, 2, and 4 h before snap freezing. RNA integrity was determined by microcapillary electrophoresis by the RNA integrity number (RIN) algorithm.

Results

Sixty-seven percent of normal colon tissues and 94 % of colon cancer specimens yielded RNA with a RIN of ≥7. Matched colon cancer and normal tissues showed significant difference in RNA quality. RNA remained stable in colon cancer tissues kept at room temperature and on ice for up to 4 h, and long-term storage of banked colon specimens did not negatively influence RNA quality (RNA with RIN of ≥7 banked less than 15 months, 83 %; 16–20 months, 78 %; 21–25 months, 77 %; 26–40 months, 90 %).

Conclusions

Frozen colon tissues yield high-quality RNA in approximately 80 % of specimens. Ex-vivo ischemia times and storage periods did not adversely affect RNA quality. This study showed that standard operation protocols and the maintenance of high-quality tissue repositories were the keys to translational medicine research.
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Metadaten
Titel
Biobanking of Fresh-frozen Human Colon Tissues: Impact of Tissue Ex-vivo Ischemia Times and Storage Periods on RNA Quality
verfasst von
Wei-Guang Bao, MD
Xia Zhang, MD
Jian-Gang Zhang, MD
Wen-Jun Zhou
Tie-Nan Bi, MD
Jiao-Chen Wang, MD, PhD
Wei-Hua Yan, MD, PhD
Aifen Lin, MD, PhD
Publikationsdatum
01.05.2013
Verlag
Springer-Verlag
Erschienen in
Annals of Surgical Oncology / Ausgabe 5/2013
Print ISSN: 1068-9265
Elektronische ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-012-2440-1

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