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Erschienen in: Annals of Surgical Oncology 3/2014

01.03.2014 | Translational Research and Biomarkers

Tumor-Associated Macrophages Promote Oral Cancer Progression Through Activation of the Axl Signaling Pathway

verfasst von: Chien-Hsing Lee, MD, PhD, Shyun-Yeu Liu, DDS, Kuo-Chou Chou, DDS, Chi-Tai Yeh, PhD, Shine-Gwo Shiah, PhD, Ren-Yeong Huang, DDS, PhD, Jen-Chan Cheng, DDS, Ching-Yu Yen, DDS, Yi-Shing Shieh, DDS, PhD

Erschienen in: Annals of Surgical Oncology | Ausgabe 3/2014

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Abstract

Background

Recent studies suggest that tumor-associated macrophages (TAMs) promote tumor growth and metastasis. Our previous report demonstrated that Axl signaling promotes carcinogenesis and progression of oral squamous cell carcinoma (OSCC). This study aims to test the potential involvement of growth arrest-specific gene 6 (Gas6)/Axl signaling in the protumoral effect of TAMs.

Methods

Co-culture experiments by incubation of OSCC cells (YD38 and OE) and macrophages (THP-1) were performed. The expression of Gas6/Axl and epithelial-mesenchymal transition (EMT) genes were examined in YD38 and OE cells. The effect of Gas6/Axl signaling on co-cultured cancer cells was further investigated by knocking down Axl expression and neutralizing Gas6. Axl and TAM distribution were analyzed by immunohistochemistry in OSCC tissues.

Results

Activation of Axl signaling and increased expression of mesenchymal markers, along with increased invasion/migration ability of OSCC cells, was noted upon co-culture with THP-1. Neutralization of Gas6 in the co-culture system or knockdown of Axl in YD38 caused the co-culture effects to be diminished. Co-culture with THP-1 increased nuclear factor (NF)-κB nuclear translocation and transcription activity in YD38 cells. A significant association between the TAM count and expression of phosphorylated Axl (P = 0.004) was found in vivo cancer tissues.

Conclusions

TAMs play a protumor role in OSCC and likely promote tumor progression through activation of the Gas6/Axl–NF-κB signaling pathway. Therefore, Gas6/Axl and NF-κB signaling in OSCC cells may be a putative target for therapeutic intervention.
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Metadaten
Titel
Tumor-Associated Macrophages Promote Oral Cancer Progression Through Activation of the Axl Signaling Pathway
verfasst von
Chien-Hsing Lee, MD, PhD
Shyun-Yeu Liu, DDS
Kuo-Chou Chou, DDS
Chi-Tai Yeh, PhD
Shine-Gwo Shiah, PhD
Ren-Yeong Huang, DDS, PhD
Jen-Chan Cheng, DDS
Ching-Yu Yen, DDS
Yi-Shing Shieh, DDS, PhD
Publikationsdatum
01.03.2014
Verlag
Springer US
Erschienen in
Annals of Surgical Oncology / Ausgabe 3/2014
Print ISSN: 1068-9265
Elektronische ISSN: 1534-4681
DOI
https://doi.org/10.1245/s10434-013-3400-0

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