Ocular melanoma is the most common primary intraocular malignancy in adults.
1 It most frequently arises from melanocytes in the uveal tract, which is subdivided in an anterior part containing the iris (~ 5%) and a posterior part containing the choroid and ciliary corpus (~ 80%).
1,
2 The rest of ocular melanomas develop in the conjunctiva (~ 5%) or elsewhere in the orbit (~ 10%). The incidence of uveal melanoma in Europe varies with latitude, being higher in Northern (≥ 8 per million) than Southern Europe (< 2 per million), due to a positive association with Caucasian ethnicity, fair skin, and light eye colour.
4 Most patients are diagnosed after age 50 years, with a peak range of 65–75 years.
1–5 Despite successful treatment of the primary tumor, up to 50% of patients will eventually develop metastatic disease with predominant liver involvement.
1–3
Metastatic ocular melanoma carries a poor prognosis, because there are no effective systemic treatments. Reported median overall survival (OS) following systemic treatment, including immunotherapy and kinase inhibitors, ranges from 4.4 to 12.7 months with a 1-year OS rate ranging from 29 to 53%.
6,
7 Meta-analyses have demonstrated that patients treated with liver-directed therapies had a significantly longer progression-free survival (PFS) and OS compared with patients receiving systemic therapy.
6,
7 Liver-directed therapies used to treat ocular melanoma liver metastases include chemoembolization, immunoembolization, radioembolization, isolated hepatic perfusion (IHP), and percutaneous hepatic perfusion with melphalan (M-PHP) (Table
1).
8–30Table 1
Summary of progression-free survival and overall survival following chemoembolization, immunoembolization, radioembolization, isolated hepatic perfusion, and percutaneous hepatic perfusion
| Phase I/II, dose-esc. | 19 | Chemoembolization (cisplatin) | N/A | 8.5 |
| Phase II | 30 | Chemoembolization (BCNU) | N/A | 5.2 |
| PS, pilot | 12 | Chemoembolization (mitomycin C) | N/A | 21 |
| RS | 25 | Chemoembolization (fotemustine/cisplatin) | 3 | 6 |
| RS | 125 | Chemoembolization (mostly cisplatina) | 3.8 | 6.7 |
| PS, pilot | 14 | Chemoembolization (cisplatin/carboplatin) | 8.5 | 11.5 |
| RS | 21 | Chemoembolization (fotemustine) | 7.3 | 28.7 |
| RS | 58 | Chemoembolization (irinotecan) | N/A | 16.5 |
| RS | 29 | Chemoembolization (cisplatin) | 6 | 23 |
| RS | 53 | Immunoembolization vs. chemoembolization (BCNU) | 12.4 vs. 4.8 | 20.4 vs. 9.8 |
| Phase II | 52 | Immunoembolization vs. bland embolization | 3.9 vs. 5.9 | 21.5 vs. 17.2 |
| RS | 32 | Radioembolization (Y-90) | 4.7 | 10 |
| RS | 13 | Radioembolization (Y-90) | N/A | 7 |
| RS | 71 | Radioembolization (Y-90) | 5.9 | 12.3 |
| RS | 16 | Radioembolization (Y-90) | 5.6 | 13.5 |
| PS | 24 | Radioembolization (Y-90) | 8.1 | 18.5 |
| Phase I/II | 22 | Isolated hepatic perfusion (melphalan) ± TNFb | 9c | 11d |
| Phase II | 29 | Isolated hepatic perfusion (melphalan) | 8 | 12.1 |
| Phase II | 8 | Isolated hepatic perfusion (melphalan) | 6.7 | 9.9 |
| Phase I/II | 8 | Isolated hypoxic hepatic perfusion (melphalan) | 6 | 11 |
| RS | 18 | Percutaneous hepatic perfusion (melphalan) | 12.4 | 9.6 |
| RS | 51 | Percutaneous hepatic perfusion (melphalan) | 8.1 | 15.3 |
| RS | 16 | Percutaneous hepatic perfusion (melphalan) | 11.1 | 27.4 |
M-PHP is a minimally invasive, repeatable technique in which the liver is isolated from the systemic circulation and subsequently perfused with high-dose chemotherapy. M-PHP is the only liver-directed therapy that has been investigated in a multicenter, randomized, controlled trial (RCT).
31 A significant improvement in hepatic and overall PFS was demonstrated in patients treated with M-PHP compared with best alternative care, but the median OS after M-PHP was only 10.6 months. Approximately 40% of patients in this study had extrahepatic metastases, and M-PHP may have had a limited effect on their OS. Additionally, 11% of patients in the study had metastases from cutaneous melanoma.
The purpose of this study was to prospectively investigate the efficacy and safety of M-PHP using the GEN 2 filter in well-selected patients with unresectable metastases from ocular melanoma confined to the liver.
Methods
This prospective, single-arm, single-center, phase II study was conducted in accordance with the Declaration of Helsinki, approved by the local ethics committee and registered on
www.trialregister.nl (NTR4112). All participants provided written informed consent.
Patients
Eligible patients were those with histologically proven, unresectable ocular melanoma metastases confined to the liver. All patients were discussed at a multidisciplinary meeting before inclusion. Exclusion criteria are listed in Table
2.
Table 2
Exclusion criteria
APTT > 1.5 × ULN | Age < 18 or > 75 yr |
PT > 1.5 × ULN | Extrahepatic disease (on CECT or FDG-PET/CT) |
Leukocytes < 3.0 × 109/L | WHO performance status ≥ 2 |
Thrombocytes < 100 × 109/L | Severe comorbidity precluding general anesthesia |
Creatinine clearance < 40 ml/min | Diabetes with nephropathy |
AST > 2.5 × ULN | Active infections |
ALT > 2.5 × ULN | < 40% healthy liver tissue |
Serum bilirubin > 1.5 × ULN | Other liver disease |
ALP > 2.5 × ULN | Vascular anatomy impeding M-PHP |
LDH > 2 × ULNa | Intracranial lesions with propensity to bleed (on CT/MRI) |
| Pregnancy |
Study Protocol
Pretreatment angiography was routinely performed approximately 1 week before the first M-PHP to evaluate hepatic arterial vasculature. If deemed necessary, hepatico-enteric shunts (e.g., right gastric and gastroduodenal artery) were embolized to prevent inadvertent leakage of melphalan.
Treatment consisted of two M-PHP procedures with hepatic artery infusion of melphalan 3 mg/kg (maximum dose 220 mg) at 6–8 weeks interval. Patients demonstrating progressive disease (PD) or unacceptable adverse events after the first M-PHP received only one procedure. If grade 3/4 hematologic toxicity occurred after the first procedure, melphalan dose was reduced by 20–25%. Patients routinely received a subcutaneous injection of granulocyte-colony stimulating factor (pegfilgrastim 6 mg) within 72 h after each M-PHP.
Contrast-enhanced CT of chest and abdomen was performed at baseline, 4–8 weeks after each M-PHP, every 3 months in the first year and every 6 months thereafter until PD occurred. MRI of the liver was performed if lesions were not or poorly visible on CT.
Quality of life (QoL) was assessed using the European Organization for Research and Treatment of Cancer Quality of Life Questionnaire version 3.0 (EORTC QLQ-C30 v3.0). Questionnaires were filled out at baseline, 6 weeks after the first and second M-PHP, and 6 months after the first M-PHP.
All adverse events were monitored continuously throughout the entire study and reported according to the Common Terminology Criteria for Adverse Events version 4.03 (CTCAE v4.03).
Procedure
All M-PHP procedures were performed using the CHEMOSAT (GEN 2) system (Delcath Systems Inc, New York). General anesthesia was performed with continuous monitoring of the central venous and arterial pressure. Access to the right internal jugular vein (IJV, 10-F sheath), right common femoral vein (CFV, 18-F sheath), and left common femoral artery (5-F sheath) was created. Heparin was administered at an initial dose of 300 U/kg and an activated clotting time of ≥ 450 s was maintained throughout the procedure. A 2.4-F or 2.7-F microcatheter was placed into the hepatic artery at the intended location of infusion. A 16-F double-balloon catheter (Isofuse Isolation Aspiration Catheter, Delcath Systems Inc, New York, NY) was placed in the inferior vena cava (IVC) via the right CFV. The cranial and caudal balloons were inflated at the atriocaval junction and infrahepatic IVC, respectively, to prohibit leakage of melphalan into the systemic circulation. The entire dose of melphalan was infused into the proper hepatic artery or split and infused in the right and left hepatic artery. Melphalan-rich blood was aspirated through catheter fenestrations in a segment between the two balloons, pumped through an extracorporeal hemofiltration system and returned to the patient via the sheath in the right IJV. Once all melphalan was administered, filtration was continued for 30 min to allow complete clearance of melphalan from the liver. The anticoagulant effects of heparin were reversed by protamine sulphate 3 mg/kg, the arterial sheath was removed and hemostasis was achieved using a closure device.
37
Endpoints
All imaging was reviewed by independent radiologists using the Response Evaluation Criteria in Solid Tumors (RECIST) 1.1 criteria.
38 Primary endpoints were overall response rate (ORR) and best overall response (BOR) according to RECIST 1.1. Secondary endpoints were best hepatic response according to RECIST 1.1, OS, PFS, hepatic progression-free survival (hPFS), safety, and QoL.
OS was defined as time of first M-PHP until death or censoring. PFS and hPFS were defined as time of first M-PHP until PD, death, or censoring.
Statistical Analysis
Kaplan–Meier estimations were used to assess OS, PFS, and hPFS. OS data were censored at the date of last follow-up if patients were still alive. The log-rank test was used to compare curves.
Cox regression analyses were performed to determine possible independent predictors for OS. The Wilcoxon signed-rank test was used to compare scores from questionnaires filled in at baseline and after treatment. P < 0.05 was considered statistically significant. Analyses were performed using SPSS 23.0 (SPSS Inc., Chicago, IL).
Discussion
This study was designed to prospectively investigate the efficacy of M-PHP with the GEN 2 filter in patients with unresectable ocular melanoma metastases confined to the liver. The ORR of 72% and survival rate (median OS 19.1 months; 1- and 2-year OS of 77% and 43%, respectively) appeared to be much longer compared to published data on other treatment modalities and provide convincing evidence for the efficacy of M-PHP.
The prognosis of patients with metastatic ocular melanoma is very poor, and there is a lack of effective systemic therapies. A meta-analysis that included 29 prospective trials that reported patients with metastatic ocular melanoma who were treated with immunotherapy, kinase inhibitors, chemotherapy, or liver-directed therapy, reported a median OS of 10.2 months, 1-year OS of 43%, and median PFS of 3.3 months.
6 Another recent meta-analysis, which included 78 peer-reviewed articles, reported similar outcomes in patients with metastatic ocular melanoma receiving either surgical, interventional radiology, or systemic treatment.
7 Median OS across all treatment modalities was 1.07 years and 1-year OS was 52%. In both meta-analyses, patients treated with liver-directed therapies had a significantly longer OS but given the paucity of RCTs the evidence is not compelling. Many studies included in the meta-analyses were retrospective cohort studies with a small sample size and differences in OS between various therapies therefore may be attributable to lead-time, selection, and publication bias.
M-PHP is the only liver-directed therapy for which efficacy was shown in an RCT by Hughes et al.
31 This trial included 93 patients with unresectable liver metastases from either ocular (
n = 83) or cutaneous (
n = 10) melanoma. Patients were randomized to M-PHP (
n = 44) or best alternative care (BAC) (
n = 49). Approximately 82% of patients in the BAC group received active treatment such as systemic chemotherapy, chemoembolization, radioembolization, and surgery. A significant improvement in hepatic and overall PFS was demonstrated in patients treated with M-PHP: 7.0 versus 1.7 months (
p < 0.0001) and 5.4 versus 1.6 months (
p < 0.0001), respectively. The gain in PFS did not result in OS benefit though. The failure to demonstrate OS benefit was most likely caused by the substantial number of patients (40%) with extrahepatic metastases, thereby limiting the optimal effect of a liver-directed therapy. Additionally, almost 60% of patients crossed over to the M-PHP group, receiving M-PHP once disease progression occurred.
The median OS of 19.1 months in the current study compares favorably to the median OS reported in the aforementioned systematic reviews and RCT. It is also longer than the median OS of 15.3 months reported in the largest retrospective study on M-PHP in patients (
n = 51) with metastatic ocular melanoma.
29 This study included patients with extrahepatic metastases if these were nonprogressive following previous treatments or amenable to ablative treatment modalities.
Clearly, our favorable survival outcomes can (partly) be attributed to the exclusion of patients with extrahepatic disease. Additionally, we excluded patients with elevated LDH levels (> 2 × ULN) at baseline, and it has been demonstrated that an elevated LDH is associated with a poor OS in patients with metastatic ocular melanoma.
6,
39,
40 Median baseline LDH level was 196 IU/L in our study versus a mean baseline LDH of 524 IU/L in the RCT by Hughes et al.
31
The hepatic response rate in our study (81%) is much higher than in the study by Hughes et al. (36%) and Karydis et al. (49%).
29,
31 The median number of M-PHP procedures that patients received under study protocol was comparable between all these three studies.
The majority of patients received some form of subsequent treatment (i.e., liver-directed therapy and/or systemic therapy) after showing PD. Although this might have influenced survival, all of these therapies were also available and used at the time of the retrospective studies by Karydis et al. (median OS 15.3 months).
29 This does not apply for the RCT by Hughes et al., which was conducted before checkpoint- and kinase inhibitors were used for metastatic ocular melanoma.
31 It is unlikely though that subsequent systemic therapies had a large impact on OS as the efficacy of systemic treatments has been limited so far.
3,
41–44
We found that the median OS in patients with a relatively long hPFS (≥ median hPFS) was significantly longer than in patients with a shorter hPFS (< median hPFS). This, together with the finding that the median OS was significantly longer in responders than nonresponders, suggests that controlling liver disease with M-PHP in patients with liver-only disease improves OS. Ideally, this should be confirmed in a phase III RCT with OS as primary endpoint and no permission for crossover. This, however, has already been proven to be difficult as the FOCUS trial (M-PHP versus best available care, NCT02678572) was recently modified into a single-arm study due to a slow inclusion rate.
We found the presence of a liver metastasis with diameter ≥ 3 cm and elevated LDH level to be poor prognostic factors for OS, as was already reported by Khoja et al.
6 We were unable to confirm their findings that an age ≥ 65 years, male sex, and elevated ALP are also poor prognostic factors for OS.
Concerns have been raised about the safety of M-PHP as prior studies reported high rates of hematologic toxicity. In previous publications, it was demonstrated that the GEN 2 filter has an improved filter extraction rate and improved safety profile.
34,
35 We now also provide evidence that M-PHP is well-tolerated with maintenance of QoL. The QoL was only mildly affected with a temporary impaired physical functioning at 6 weeks after the second M-PHP.
The majority of patients (74%) developed extrahepatic metastatic disease during follow-up. These may have been new metastases that developed after M-PHP or metastases that were radiologically occult at baseline. This indicates that many patients with ocular melanoma will suffer from systemic spread for which liver-directed therapy is only a temporarily treatment solution. We recently started a phase I/II study investigating combination therapy of M-PHP with ipilimumab/nivolumab in order to better control both hepatic and extrahepatic disease (CHOPIN trial, NCT04283890). Results of trials investigating the efficacy of check-point inhibitors alone have been disappointing in patients with ocular melanoma metastases. Ocular melanoma cancer cells carry a low tumor mutational burden, which is thought to decrease the likelihood of neoantigen presentation necessary to evoke antitumoral response by T-cells.
45 Tumor lysis and necrosis induced by M-PHP could potentially provoke antigen release that may stimulate cancer-specific immune response and increase the efficacy of check-point inhibitors.
Our study had several limitations. First, this was a single-arm study with a relatively small sample size. Second, we studied a selected group of patients by applying multiple specific exclusion criteria such as the presence of extrahepatic disease, elevated LDH level, and patient age. The relatively high median OS could therefore partly be attributed to selection.
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