Noninvasive Measurement of Cerebral Blood Flow and Glucose Metabolic Rate in the Rat with High-Resolution Animal Positron Emission Tomography (PET) : A Novel in Vivo Approach for Assessing Drug Action in the Brains of Small Animals

Yasuhiro MAGATA; Hideo SAJI; SeokRye CHOI; Ken TAJIMA; Takeshi TAKAGAKI; Satoshi SASAYAMA; Yoshiharu YONEKURA; Haruhiro KITANO; Mitsuo WATANABE; Hiroyuki OKADA; Etsuji YOSHIKAWA; Takaji YAMASHITA; Akira YOKOYAMA; Junji KONISHI

doi:10.1248/bpb.18.753

Yasuhiro MAGATA, Hideo SAJI, SeokRye CHOI, Ken TAJIMA, Takeshi TAKAGAKI, Satoshi SASAYAMA, Yoshiharu YONEKURA, Haruhiro KITANO, Mitsuo WATANABE, Hiroyuki OKADA, Etsuji YOSHIKAWA, Takaji YAMASHITA, Akira YOKOYAMA, Junji KONISHI

Author information

Yasuhiro MAGATA
Hideo SAJI
SeokRye CHOI
Ken TAJIMA
Takeshi TAKAGAKI
Satoshi SASAYAMA
Yoshiharu YONEKURA
Haruhiro KITANO
Mitsuo WATANABE
Hiroyuki OKADA
Etsuji YOSHIKAWA
Takaji YAMASHITA
Akira YOKOYAMA
Junji KONISHI
Author's Organization：
Department of Nuclear Medicine, School of Medicine, Kyoto University
Department of Radiopharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University
Department of Radiopharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University
Department of Radiopharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University
Department of Radiopharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University
Department of Nuclear Medicine, School of Medicine, Kyoto University
Department of Brain Pathophysiology, School of Medicine, Kyoto University
Department of Nuclear Medicine, School of Medicine, Kyoto University
Hamamatsu Photonics K. K.
Hamamatsu Photonics K. K.
Hamamatsu Photonics K. K.
Hamamatsu Photonics K. K.
Department of Radiopharmaceutical Chemistry, Faculty of Pharmaceutical Sciences, Kyoto University
Department of Nuclear Medicine, School of Medicine, Kyoto University

Keywords: animal positron emission computed tomography, ^<18>F-2-fluoro-2-deoxy-glucose, ^<15>O-water, glucose cerebral metabolic rate, cerebral blood flow, rat brain

JOURNAL FREE ACCESS

1995 Volume 18 Issue 5 Pages 753-756

DOI https://doi.org/10.1248/bpb.18.753

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Abstract

The cerebral metabolic rate of glucose (CMRglc) and cerebral blood flow (CBF) in rats were estimated noninvasively with a high-resolution animal positron emission tomography (PET) system that we recently developed. Quantifications of CMRglc and CBF were attempted with radiotracers, ¹⁸F-2-fluoro-2-deoxyglucose and ¹⁵O-water, respectively. From the data obtained with PET, the CMRglc and CBF values under pretreatment conditions were respectively calculated as 51±14μmol/min/100g and 61±7ml/min/100g under pentobarbital-anesthesia. These values were in close agreement with published data obtained with the autoradiographic technique. Moreover, and increase of 69% (mean) in CBF was detected after i.v. acetazolamide administration. The results indicate that this high-resolution animal PET scanner holds great potential for the assessment of drug-related metabolic and circulatory effects in intact and experimental animals.

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