Hypouricemic Effects of Acacetin and 4,5-O-Dicaffeoylquinic Acid Methyl Ester on Serum Uric Acid Levels in Potassium Oxonate-Pretreated Rats

Mai Thanh Thi Nguyen; Suresh Awale; Yasuhiro Tezuka; Liying Shi; Syed Faisal Haider Zaidi; Jun-ya Ueda; Quan Le Tran; Yukihisa Murakami; Kinzo Matsumoto; Shigetoshi Kadota

doi:10.1248/bpb.28.2231

Regular Articles

Hypouricemic Effects of Acacetin and 4,5-O-Dicaffeoylquinic Acid Methyl Ester on Serum Uric Acid Levels in Potassium Oxonate-Pretreated Rats

Mai Thanh Thi Nguyen, Suresh Awale, Yasuhiro Tezuka, Liying Shi, Syed Faisal Haider Zaidi, Jun-ya Ueda, Quan Le Tran, Yukihisa Murakami, Kinzo Matsumoto, Shigetoshi Kadota

Author information

Mai Thanh Thi Nguyen
Division of Natural Products Chemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
Suresh Awale
Division of Natural Products Chemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
Yasuhiro Tezuka
Division of Natural Products Chemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
Liying Shi
Division of Natural Products Chemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
Syed Faisal Haider Zaidi
Division of Natural Products Chemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
Jun-ya Ueda
Division of Natural Products Chemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University
Quan Le Tran
National University-Hochiminh City, Vietnam
Yukihisa Murakami
Division of Medicinal Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Universit
Kinzo Matsumoto
Division of Medicinal Pharmacology, Institute of Natural Medicine, Toyama Medical and Pharmaceutical Universit
Shigetoshi Kadota
Division of Natural Products Chemistry, Institute of Natural Medicine, Toyama Medical and Pharmaceutical University

Keywords: hypouricemia, uric acid, acacetin, 4,5-O-dicaffeoylquinic acid methyl ester, xanthine oxidase inhibition

JOURNAL FREE ACCESS

2005 Volume 28 Issue 12 Pages 2231-2234

DOI https://doi.org/10.1248/bpb.28.2231

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Abstract

The effects of acacetin (1) and 4,5-O-dicaffeoylquinic acid methyl ester (2), compounds contained in the flowers of Chrysanthemum sinense SABINE, on the serum uric acid level were investigated using the rats pretreated with the uricase inhibitor potassium oxonate as an animal model for hyperuricemia. When administered per orally at doses of 20 and 50 mg/kg, 1 reduced the serum uric acid level by 49.9 and 63.9%, respectively and 2 reduced the level by 31.2 and 44.4%, respectively. On the other hand, when the same doses were given intraperitoneally, both of compounds also exhibited a dose-dependent and more marked reduction of the serum uric acid level (% reduction at 20 and 50 mg/kg were 63.0 and 95.1% in 1, respectively and 66.9 and 86.5% in 2, respectively). Furthermore, the compounds 1 and 2 inhibited the rat liver xanthine oxidase activity with IC₅₀ values of 2.22 μM and 5.27 μM, respectively. These results demonstrated the hypouricemic action of 1 and 2, which may be attributable to their xanthine oxidase inhibitory activity.

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