Biological and Pharmaceutical Bulletin
Online ISSN : 1347-5215
Print ISSN : 0918-6158
ISSN-L : 0918-6158
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Inhibition of the Epstein–Barr Virus Lytic Cycle by Andrographolide
Tsuey-Pin LinShih-Ying ChenPin-Der DuhLi-Kwan ChangYen-Ni Liu
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2008 Volume 31 Issue 11 Pages 2018-2023

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Abstract

Andrographis paniculata NEES is a medicinal plant that is commonly used in Asia. This work demonstrates that 25 μg/ml of ethanolic extract from A. paniculata (EEAP) and 5 μg/ml of andrographolide, a bioactive compound in EEAP, effectively inhibit the expression of Epstein–Barr virus (EBV) lytic proteins, Rta, Zta and EA-D, during the viral lytic cycle in P3HR1 cells. Transient transfection analysis revealed that the lack of expression of Rta, Zta and EA-D is caused by the inhibition of the transcription of BRLF1 and BZLF1, two EBV immediate-early genes that encode Rta and Zta, respectively. This study finds that the inhibition prevents the virus from producing mature viral particles. Meanwhile, andrographolide is not toxic to P3HR1 cells when the concentration is below 5 μg/ml, indicating that the compound is potentially useful as an anti-EBV drug.

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© 2008 The Pharmaceutical Society of Japan
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