Six steroidal glycosides were isolated from the fresh leaves of Aspidistra elatior BLUME (Liliaceae) and the structures of these glycosides, tentatively named glycosides A (1), B (2), C (3), D (4), E (5) and F (6), were established to be neopentologenin 5-O-β-D-glucopyranoside (1), 26-O-β-D-glucopyranosyl 22-methoxy-5β-furostane-1β, 3β, 4β, 5β, 26-pentaol 5-O-β-D-glucopyranoside (2), aspidistrin (3), 26-O-β-D-glucopyranosyl 22-methoxy-5β-furostane-1β, 2β, 3β, 4β, 5β, 26-hexaol 5-O-β-D-glucopyranoside (4), methyl proto-aspidistrin (5) and magnesium 26-O-β-D-glucopyranosyl 22-methoxy-5β-furostane-1β, 3β, 4β, 5β, 26-pentahydroxy-2β-yl-sulfate monohydroxide (6), respectively. The last compound is the first sulfated steroidal glycoside to be isolated from a Liliaceous plant.