Formononetin (1), isoliquiritigenin (2), echinatin (3), liquiritigenin (4), p-hydroxybenzoic acid (5), 3'-hydroxyformononetin (6) and isobavachalcone (7) were isolated from callus culture of Glycyrrhiza uralensis which was established on Murashige-Skoog's medium containing naphthaleneacetic acid (NAA, 2ppm), (2, 4-dichlorophenoxy) acetic acid (2, 4-D, 1ppm) and benzyladenine (0.1ppm). Formononetin (1), 3'-hydroxyformononetin (6) and isobavachalcone (7) showed different patterns of production.