The rhizome of Veratrum stamineum Maxim. was extracted by the ammonia-ether-chloroform method and the alkaloids were isolated by the utilization of the difference in the solubility of the hydrochloride and the sulfate. Jervine, veratramine, and the so-called amorphous base were obtained in the respective yield of approximately 0.1%, 0.025%, and 0.28%.