Inhibition of Cell Growth and Induction of Apoptosis via Inactivation of NF-κB by a Sulfurcompound Isolated From Garlic in Human Colon Cancer Cells

Jung Ok Ban; Dong Yeon Yuk; Koan Sik Woo; Tae Myoung Kim; Ung Soo Lee; Heon-Sang Jeong; Dae Joong Kim; Yeun Bok Chung; Bang Yeon Hwang; Ki Wan Oh; Jin Tae Hong

doi:10.1254/jphs.FP0070789

Full Papers

Inhibition of Cell Growth and Induction of Apoptosis via Inactivation of NF-κB by a Sulfurcompound Isolated From Garlic in Human Colon Cancer Cells

Jung Ok Ban, Dong Yeon Yuk, Koan Sik Woo, Tae Myoung Kim, Ung Soo Lee, Heon-Sang Jeong, Dae Joong Kim, Yeun Bok Chung, Bang Yeon Hwang, Ki Wan Oh, Jin Tae Hong

Author information

Jung Ok Ban
College of Pharmacy, Chungbuk National University, Korea
Dong Yeon Yuk
College of Pharmacy, Chungbuk National University, Korea
Koan Sik Woo
College of Agriculture, Life and Enviroments Sciences, Chungbuk National University, Korea
Tae Myoung Kim
College of Veternary Medicine, Chungbuk National University, Korea
Ung Soo Lee
Department of Food and Biotechnology, Chungju National University, Korea
Heon-Sang Jeong
College of Agriculture, Life and Enviroments Sciences, Chungbuk National University, Korea
Dae Joong Kim
College of Veternary Medicine, Chungbuk National University, Korea
Yeun Bok Chung
College of Pharmacy, Chungbuk National University, Korea
Bang Yeon Hwang
College of Pharmacy, Chungbuk National University, Korea
Ki Wan Oh
College of Pharmacy, Chungbuk National University, Korea
Jin Tae Hong
College of Pharmacy, Chungbuk National University, Korea

Keywords: thiacremonone, sulfurcompound, nuclear transcription factor-κB (NF-κB), colon cancer, apoptosis

JOURNAL FREE ACCESS

2007 Volume 104 Issue 4 Pages 374-383

DOI https://doi.org/10.1254/jphs.FP0070789

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Abstract

Compounds such as S-allylmercaptocysteine, diallyl disulfide, and S-trityl-L-cysteine isolated from garlic have been known to be effective in chemoprevention. Nuclear transcription factor-κB (NF-κB) has been known to be an implicated factor in apoptotic cell death of several cancer cells. In this study, we investigated whether a sulfurcompound (named thiacremonone) isolated from garlic could modulate NF-κB activity and thereby induce apoptotic cell death of colon cancer cells. Treatment with different concentrations (30 – 150 μg/ml) of thiacremonone for various periods (0 – 48 h) inhibited colon cancer cell (SW620 and HCT116) growth followed by induction of apoptosis in a dose-dependent manner. We also found that thiacremonone modulated tumor necrosis factor-α (TNF-α) and tetradeanoyl phorbol acetate (TPA)-induced NF-κB transcriptional and DNA binding activity. Moreover, thiacremonone suppressed NF-κB target anti-apoptotic genes (Bcl-2, cIAP1/2, and XIAP) and inflammatory genes (iNOS and COX-2), whereas it induced apoptotic genes (Bax, cleaved caspse-3, and cleaved PARP) expression. These results suggest that a novel sulfurocompound from garlic inhibited colon cancer cell growth through induction of apoptotic cell death by modulating of NF-κB.

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