H2O2 tolerance of Vibrio rumoiensis S-1T is attributable to the cellular catalase activity
Section snippets
Bacterial strains
The strain examined was V. rumoiensis strain S-1T, which exhibits a high level of catalase activity. Strain S-4 was used as the catalase-deficient strain. Strain S-4 was isolated during repeated cultivations in the early stage of strain S-1T isolation. These strains were cultivated aerobically at 27°C in PYS-2 broth or agar slant (containing 1.5% agar) (pH 7.5) containing (per liter of deionized water) 8.0 g of polypeptone (Nihon Pharmaceutical, Tokyo), 3.0 g of yeast extract (Kyokuto, Tokyo),
Systematic characterization of catalase-deficient strain S-4
Strain S-4 was isolated from the storage culture at 4°C in PYS agar slant (10) using artificial seawater instead of deionized water and NaCl. The 16S rRNA gene sequence (1468 bases) of strain S-4 was analyzed to confirm if the strain was identical to strain S-1T at the species level by sequence similarity. Almost the entire 16S rRNA gene sequence (GenBank/EMBL/DDBJ accession no. AB218691) of strain S-4 was identical to that of strain S-1T (accession no. AB013297). The combined results of the
Discussion
It has been reported that the H2O2 tolerance of strain S-1T is attributable to the catalase (VktA) released from disrupted cells in the part of the population of this strain damaged by H2O2 (12). Cell disruption has been observed during the inoculation of strain S-1T into 5 mM H2O2-containing medium (12). In this study, apparent cell disruption was not observed even when 100 mM H2O2 was added to the culture medium in the mid-exponential growth phase. This discrepancy in results might be due to
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