Neuronal Degeneration and Microglial Activation in the Ischemic Dentate Gyrus of the Gerbil

Joong Bum MOON; Choong Hyun LEE; Chan Woo PARK; Jun Hwi CHO; In Koo HWANG; Ki-Yeon YOO; Jung Hoon CHOI; Hyung-Cheul SHIN; Moo-Ho WON

doi:10.1292/jvms.001381

Anatomy

Neuronal Degeneration and Microglial Activation in the Ischemic Dentate Gyrus of the Gerbil

Joong Bum MOON, Choong Hyun LEE, Chan Woo PARK, Jun Hwi CHO, In Koo HWANG, Ki-Yeon YOO, Jung Hoon CHOI, Hyung-Cheul SHIN, Moo-Ho WON

Author information

Joong Bum MOON
Department of Emergency Medicine, College of Medicine, Kangwon National University
Choong Hyun LEE
Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University
Chan Woo PARK
Department of Emergency Medicine, College of Medicine, Kangwon National University
Jun Hwi CHO
Department of Emergency Medicine, College of Medicine, Kangwon National University
In Koo HWANG
Department of Anatomy and Cell Biology, College of Veterinary Medicine and BK21 Program for Veterinary Science, Seoul National University
Ki-Yeon YOO
Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University
Institute of Natural Medicine, College of Medicine, Hallym University
Jung Hoon CHOI
Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University
Institute of Natural Medicine, College of Medicine, Hallym University
Hyung-Cheul SHIN
Department of Physiology, College of Medicine, Hallym University
Institute of Natural Medicine, College of Medicine, Hallym University
Moo-Ho WON
Department of Anatomy and Neurobiology, and Institute of Neurodegeneration and Neuroregeneration, College of Medicine, Hallym University
Institute of Natural Medicine, College of Medicine, Hallym University

Keywords: dentate gyrus, gerbil, ischemia/reperfusion, microglia, neuronal degeneration

JOURNAL FREE ACCESS

2009 Volume 71 Issue 10 Pages 1381-1386

DOI https://doi.org/10.1292/jvms.001381

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Abstract

In the present study, we investigated the time-course changes of neuronal degeneration and microglial activation in the gerbil dentate gyrus after transient cerebral ischemia using Fluoro-Jade B histofluorescence staining and immunohistochemistry for Iba-1. Fluoro-Jade B positive cells were observed from 6 hr and markedly increased 1 day after ischemia/reperfusion. Iba-1-immunoreactive microglia were increased and hypertrophied at early time, and Iba-1 immunoreactivity was highest at 2 days after ischemia/reperfusion. These results may be direct evidence on neuronal degeneration and microglial activation in the gerbil dentate gyrus after ischemia/reperfusion.

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