Role of allogenic mesenchymal stem cells in the reconstruction of bone defect in rabbits

  • Hadisoebroto D. Ismail Department of Orthopaedic and Traumatology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Phedy Phedy Department of Orthopaedy and Traumatology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Erica Kholinne Department of Orthopaedy and Traumatology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Achmad A. Jusuf Department of Histology, Faculty of Medicine, Universitas Indonesia, Jakarta
  • Nyimas D. Yulisa Department of Radiology, Faculty of Medicine, Universitas Indonesia, Jakarta
DOI: https://doi.org/10.13181/mji.v23i1.683
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Abstract

Background: Despite the advancement of bone reconstruction in the past decade, large bone defect remains a challenge for orthopedic surgery. As mesenchymal stem cells (MSCs) emerges as one of the possible treatment of these defects, we evaluate the effect of its transplantation, particularly in combination with hydroxyapatite-calcium sulphate pellets.

Methods: Twenty eight rabbits were randomly assigned into four different treatment groups. Each group received a different type of grafts (Autograft, hydroxyapatite-calcium sulphate [HA-CaSO4], HA-CaSO4 combined with marrow aspirate, or HA-CaSO4 combined with 2x106 MSCs). One centimeter long bone defects were created then immediately fixated with mini plate-screw and two cerclage wires. It was followed by the graft transplantation. Callus thickness was measured from the x-rays taken at 4, 8, 12 week after transplantation by two authors working independently. At the end of the study, histological staining along with osteocyte index were obtained by sacrificing the rabbits. These data were analyzed by one-way ANOVA test.

Results: At the fourth week, callus thickness showed significant difference (p = 0.018). Although statistically insignificant, callus in MSCs group at the eighth week seemed to be thicker than any other groups of intervention (p = 0.546). The MSCs group also tend to have a higher osteocyte index at the follow-up weeks.

Conclusion: MSC transplantation on bone defect results in faster callus formation and tends to generate a thicker callus.

Keywords: bone defect, callus thickness, hydroxyapatite-calcium sulphate, mesenchymal stem cells, osteocyte index

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Published
2014-03-11
How to Cite
1.
Ismail HD, Phedy P, Kholinne E, Jusuf AA, Yulisa ND. Role of allogenic mesenchymal stem cells in the reconstruction of bone defect in rabbits. Med J Indones [Internet]. 2014Mar.11 [cited 2024Apr.20];23(1):9-14. Available from: http://mji.ui.ac.id/journal/index.php/mji/article/view/683
Issue
Vol. 23 No. 1 (2014): February
Section
Basic Medical Research

Copyright (c) 2014 Hadisoebroto D. Ismail, Phedy Phedy, Erica Kholinne, Achmad A. Jusuf, Nyimas D. Yulisa

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