Figures
Abstract
Background
In line with the Global trend to improve malaria control efforts a major campaign of insecticide treated net distribution was initiated in 1999 and indoor residual spraying with DDT or pyrethroids was reintroduced in 2000 in Zambia. In 2006, these efforts were strengthened by the President's Malaria Initiative. This manuscript reports on the monitoring and evaluation of these activities and the potential impact of emerging insecticide resistance on disease transmission.
Methods
Mosquitoes were captured daily through a series of 108 window exit traps located at 18 sentinel sites. Specimens were identified to species and analyzed for sporozoites. Adult Anopheles mosquitoes were collected resting indoors and larva collected in breeding sites were reared to F1 and F0 generations in the lab and tested for insecticide resistance following the standard WHO susceptibility assay protocol. Annual cross sectional household parasite surveys were carried out to monitor the impact of the control programme on prevalence of Plasmodium falciparum in children aged 1 to 14 years.
Results
A total of 619 Anopheles gambiae s.l. and 228 Anopheles funestus s.l. were captured from window exit traps throughout the period, of which 203 were An. gambiae malaria vectors and 14 An. funestus s.s.. In 2010 resistance to DDT and the pyrethroids deltamethrin, lambda-cyhalothrin and permethrin was detected in both An. gambiae s.s. and An. funestus s.s.. No sporozoites were detected in either species. Prevalence of P. falciparum in the sentinel sites remained below 10% throughout the study period.
Conclusion
Both An. gambiae s.s. and An. funestus s.s. were controlled effectively with the ITN and IRS programme in Zambia, maintaining a reduced disease transmission and burden. However, the discovery of DDT and pyrethroid resistance in the country threatens the sustainability of the vector control programme.
Citation: Chanda E, Hemingway J, Kleinschmidt I, Rehman AM, Ramdeen V, Phiri FN, et al. (2011) Insecticide Resistance and the Future of Malaria Control in Zambia. PLoS ONE 6(9): e24336. https://doi.org/10.1371/journal.pone.0024336
Editor: Roly D. Gosling, London School of Hygiene and Tropical Medicine, United Kingdom
Received: April 15, 2011; Accepted: August 4, 2011; Published: September 6, 2011
Copyright: © 2011 Chanda et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Funding: This study was financially supported by the Innovative Vector Control Consortium (IVCC) and the Health Services and Systems Programme, Zambia. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.
Competing interests: The authors have declared that no competing interests exist.
Introduction
Malaria vector control activities are substantially increasing in many malaria endemic countries [1], with some countries considering elimination [2]. Indoor Residual Spraying (IRS) and Insecticide Treated Nets (ITNs) form the backbone of these activities and both have been proven as excellent vector control strategies [3]. However, the number of insecticides recommended for both methods is severely restricted, hence monitoring and management of insecticide resistance within the control programme is essential if control is to be maintained [4].
Zambia first initiated IRS with DDT in the 1950s, at the same time malaria became a notifiable disease [5]. IRS coverage was reduced by 30% by 1973 due to economic constraints and environmental concerns about the use of DDT. The IRS programme stopped completely in the mid 1980s. With reduced vector control and the development of parasite resistance to anti-malarial drugs [6], malaria cases increased from 121.5 per 1000 in 1976 to 394 cases per 1000 in 2002[5].
In 2000, Konkola Copper Mines, a private company, reintroduced IRS with pyrethroids and DDT in two districts in Zambia [7] The success of these IRS programmes led the National Malaria Control Programme (NMCP) to again implement an IRS programme alongside the distribution of Long Lasting Insecticide Treated Nets (LLINs) [8]. As these malaria vector control interventions are being scaled up their impact has been monitored [9].
In areas of hyper and holoendemic malaria, vector control is critical in reducing human malaria transmission and the related morbidity and mortality. Evidence-based deployment and optimal assessment of vector control interventions is needed to formulate operational control policies. This requires the collation and assessment of a number of different data systems. To facilitate data handling the Zambian NMCP evaluated the Malaria Decision Support System (MDSS) developed by the Innovative Vector Control Consortium (IVCC) [10].
Materials and Methods
Ethics clearance
Ethical clearance for this study was obtained from the University of Zambia Biomedical Ethical Committee (Assurance No. FWA00000338, IRB00001131 of IOR G0000774 reference code 002-07-07). Written informed consent was obtained from all participants in this study.
Study sites and interventions
Zambia is situated in the Southern African region with a population of approximately 13 million, 45% of whom are below the age of fifteen [11]. Data were collected from 18 sentinel sites, distributed amongst nine districts within a 350 km radius of the capital, Lusaka (Figure 1). Sites were selected to assist in monitoring the expanding vector control programme. At each site annual household surveys were carried out to measure Plasmodium falciparum prevalence in children aged 1 to 14. Relative mosquito abundance and infectivity were measured as a proxy for transmission and insecticide resistance status was monitored. Indoor residual spraying was carried out in October each year from 2003 to 2010 with DDT at 2 g/m2 (Avima, South Africa) in 5 sites only (Chimoto, Kabulongo, Kafue Estates, Mufweshya and Mukobeko). Perma Net®, (Verstargaard) and Olyset® (Sumitomo), LLINs were distributed to all areas (Figure 1). Countrywide mass distribution of ITNs commenced in 2005, prior to this ITN distribution was through the antenatal clinics and commercial outlets. Over 5 million ITNs, enough to cover 96% of Zambia population, had been distributed by 2008 [8,9]. All vector control was carried out by the NMCP as part of their ongoing operational control activities.
Plasmodium falciparum prevalence surveys
Prevalence surveys were conducted at the end of the malaria transmission season in April/May in 2008, 2009 and 2010. Households were selected from strata formed by dividing sentinel sites into quadrants. In each quadrant a starting house was selected and all children aged 1 to 14 tested for P. falciparum infection using ICT™ malaria combo rapid diagnostic tests (R&R, Cape Town, South Africa). The investigator then proceeded to the next nearest house and tested all children until a total of 35 children per quadrant had been tested. Children testing positive for P. falciparum were offered treatment with Coartem® (artemether-lumefantrine) according to the National Malaria Control Programme (NMCP) guidelines. Any complicated malaria case was referred to the nearest health centre. The sentinel site specific sample size was calculated to provide evidence at the 5% significance level of an absolute reduction in P. falciparum prevalence of 20%. Prevalence of P. falciparum infection and 95% confidence intervals (CI) for each sentinel site were estimated taking account of clustering by sentinel site using the statistical software package STATA (StataCorp LP. Stata Statistical Software: Release 10. College Station, TX, USA.).
Sentinel sites were considered as the primary sampling unit. Logistic regression, allowing for complex survey designs, was performed to estimate the mean effect of the vector control intervention on prevalence compared to 2008 data.
Mosquito Species Abundance and Infectivity
Window exit traps were fitted to six houses in each sentinel site with the homeowners consent in April 2008 and operated until May 2010. Houses were selected to cover the whole of a sentinel site. Each house was constructed in traditional style and in good repair with a window in the bedroom for the exit trap to be fitted. The Chunga sentinel site was not used for exit window trap collections, as houses were constructed from concrete blocks with corrugated metal roofs. Mosquito collections were made daily by homeowners trained to empty the contents of the window trap into pre-labelled specimen jars containing isopropanol. Checklists were completed specifying nights for which traps were not operating.
Mosquito species identification
Anopheline mosquitoes were identified morphologically as Anopheles gambiae Giles complex, An. funestus Giles group or An. nili Theobald group [12,13]. Sibling species were identified and An. gambiae s.s were identified as M or S molecular form using PCR [14–18].
Malaria transmission
DNA was extracted from the head and thorax of mosquitoes and tested for the presence of P. falciparum sporozoites [19]. Numbers of mosquitoes per trap per night were calculated for each vector species. Using the species specific estimated sporozoite prevalence the number of infectious mosquitoes per species per trap per night was calculated. The relative transmission index, defined as the ratio of infective numbers of mosquitoes per trap per night, relative to the baseline year of 2008, could not be computed because none of the specimens collected contained sporozoites [20].
Insecticide Resistance
Mosquitoes were collected during the rainy seasons of 2009 and 2010 from 17 localities. Anopheles mosquitoes were collected between 06.00 and 08.00 either by larval collections from breeding sites, or as blood fed adult females resting inside homes. Collected mosquitoes were transported to the laboratory, and reared to adults or transferred to individual oviposition tubes and females allowed to lay eggs that were reared to adults at 26±2°C and 70–80% relative humidity.
Insecticide susceptibility assays were carried out on a random sample of 1-3 day old, sugar fed F1 and F0 adults following the procedure described by the World Health Organization (WHO 1998). Insecticides tested included bendiocarb (0.01%), DDT (4%), deltamethrin (0.05%), lambda-cyhalothrin (0.05%), malathion (5%), permethrin (0.75%), propoxur (0.1%).
Both Leu-Phe (west) and Leu-Ser (east) kdr mutations were investigated [21,22] using the taqman PCR [23].
Results
Malaria Prevalence in Children 1 to 14 years old
A total of 1,823 children aged between 1 to 14 years were tested for P. falciparum parasitaemia in all sentinel sites except Manueli and Nyamankalo in April/May 2008. Follow-up surveys were carried out in all sites in 2009 and 2010 with 2,176 children and 2,192 children respectively. The combined prevalence of infection with P. falciparum across all sites was 6.9% (95% CI = 3.9–12.1) in 2008, 4.9% in 2009 (95% CI 1.7–13.4) and 6.8% in 2010 (CI = 2.2–18.9) (Table 1).
No significant change in prevalence was observed in all ITN sites between years 2008, 7.2 (95% CI = 3.3–15.2), 2009, 5.9 (CI 95% = 1.9–17.3) to 2010, 7.9 (95% CI = 2–26.3). In IRS sites there was a significant overall decline in prevalence of infection between 2008 and 2009 from 6.3 (95% CI = 3.4–11.5) to 1.8 (95% CI = 0.4–8.4) (OR = 0..28, 95% CI = 0.05–1.44, P = 0.036). However, prevalence increased again in 2010 to 4.0 (95% CI = 2.47–5.47) (OR = 2.22, 95% CI = 0.57–8.65, P = 0.031) (Table 1).
Mosquito Species Abundance, sporozoite rate and transmission
A total of 619 An. gambiae s.l. and 228 An. funestus s.l. were collected and morphologically identified from 108 window exit traps operating continuously from April 2008 to May 2010. Five hundred and twenty five An. gambiae s.l. were subsequently identified to species level. There were four An. gambiae s.s, 199 An. arabiensis and 322 An. quadriannulatus. Two hundred and four An. funestus s.l were identified to species, these were 14 An. funestus s.s, 98 An. parensis, 20 An. rivulorum, 18 An. leesoni, 16 An. vaneedeni, 14 were identified as the recently described An. funestus-like, 1 An.nili s.s and 23 unidentified. Culicine mosquitoes were recorded in the absence of Anopheles to demonstrate that the traps were being operated correctly.
At 217, the numbers of actual vectors, An. gambiae s.s., An. arabiensis and An. funestus s.s. trapped was extremely low. All of these were tested for sporozoites and all were negative. Due to zero sporozoites a transmission index could not be calculated (Table 2).
Insecticide susceptibility
A total of 1,742 An. gambiae s.s. and 796 An. funestus s.s., from 17 localities, 11 of which were sentinel sites were assayed for insecticide susceptibility using the WHO protocol [24]. Prior to 2009, no resistance had been detected to any insecticide in An. gambiae s.l. or An. funestus in Zambia (Table 3 and Table 4). Between 2009 and 2010 resistance to pyrethroids (deltamethrin, lambda-cyhalothrin and permethrin) and DDT was detected in both An. gambaie s.s.and An. funestus s.s. No resistance was detected to the carbamate (bendiocarb) or the organophosphate (malathion) in either species. Limited numbers of mosquitoes were found at all sites, which correlates with the species abundance calculated from the window trap collections.
Prior to interventions mosquitoes from tested from 8 locations in Zambia were fully susceptible to the WHO discriminating dosage of deltamethrin, lambda-cyhalothrin, DDT, propoxur and malathion in 1999. Three of these locations, Chipulukusu, Mukobeko and Mushili had IRS introduced in 2003. In 2010, significant (P<0.001) resistance was detected in An. gambiae s.s.in Chipulukusu to deltamethrin (13.5%), permethrin (61%) and DDT (43%) and Mushili to DDT (55%). In Mukobeko no resistance was detected in An. gambiae s.s. to deltamethrin but a slight increase in resistance was detected in An. funestus s.s. (96% P<0.1). In Chibombo where only ITNs have been distributed resistance to deltamethrin was detected in An. funestus s.s. (88.9% P<0.001) in 2010 (Table 3 & 4 and Figure 2).
In 2010 new sites were surveyed for resistance. In the IRS sites An. funestus s.s. was susceptible to deltamethrin and DDT in Kabulongo, Katete and Mufweshya, but had low level resistance to deltamethrin (95.6%) and DDT (98%) in Kafue. Resistance to DDT was (3.8%) and deltamethrin (95.2%) was detected in An. gambiae s.s. from Kizingezinge.
Similarly in 2010 nine new sites that had only received ITNs as interventions were surveyed for insecticide resistance. In An. gambiae s.s. resistance to deltamethrin ranged from 41.8–100% in these sites with resistance to lambda-cyhalothrin being detected in Nyamankalo (83.3%) and DDT in Myooye (69%). Similarly a range of resistance was detected to deltamethrin in An. funestus s.s. (66.6–100%), permethrin resistance was also detected in Nanga Farms (90.9%) and to DDT in Myooye (94%) and Nyamankalo (88%) (Table 3 &4).
Molecular forms and kdr mutations
In four localities with pyrethroid and DDT resistant An. gambiae and one (Chipepo) with high pyrethroid resistance, PCR assays, to check for the presence of east (leu-ser) (Ranson et al. 2000) and west (leu-phe) (Martinez-Torres et al. 1998) kdr mutations were carried out on a random sample. All 159 An. gambiae s.s were identified as the molecular S-form and all were negative for east kdr. In four of the sites, Chipepo, Chipulkusu, Kizhingezhinge and Mushili all samples carried the west kdr mutation. In Myooye, only one sample was homozygous for west kdr (Table 5).
Discussion
The Roll Back Malaria, United Nations Millennium Development Goals and World Health Assembly universal access and coverage targets for malaria prevention and treatment have been established to stimulate the reduction in disease transmission. To meet these targets malaria control interventions are now being scaled up [25,26]. In order to obtain and maintain these goals there is a need for continuous surveillance, monitoring and evaluation of malaria control programmes to make informed decisions and guide control efforts.
In Zambia, the initial re-deployment of vector control interventions, ITN (1999) and IRS (2000), was based on minimal empirical evidence. Subsequent monitoring and evaluation has allowed more informed decisions to be made on targeting these interventions. Zambia has achieved high coverage of IRS (20% of households) and ITNs (60% plus of households) as of 2008 [9], and now needs to sustain these efforts.
Prevalence of parasites in children has been frequently used as a surrogate measure for malaria transmission intensity [27]. Previous studies conducted in Zambia, prior to or early into the re-introduction of interventions, as population based surveys, or from hospital based routine surveillance show widely heterogeneous results [7,28,29]. In this study, the overall average P. falciparum prevalence in children between the ages of 1 and 14 years was below 10% implying low transmission. This low prevalence has been achieved in part due to effective vector control interventions. Between 2003 and 2008 approximately 5.9 million ITNs (since 2007 all have been LLINs) have been distributed and IRS expanded from 5 to 36 districts, increasing the number of structures sprayed from less than 100,000 to over 1 million [9]. At the same time Zambia expanded clinical control of malaria through improved diagnostics (microscopy and RDTs), introducing ACTs into all clinics and expanding the network of community health workers.
Species density and infectivity measures are important indicators of malaria transmission [20,30]. The endophilic nature of An. funestus s.s. and An. gambiae s.s makes these species susceptible to both IRS and ITNs, which reduce abundance and sporozoite rates [20,31]. Anopheles arabiensis on the other hand is generally exophilic and may be the cause of underlying transmission in Zambia, a topic that warrants further investigation. In this study, both IRS and ITNs are associated with a lower abundance of An. gambiae s.s, and An. funestus s.s. than An. arabiensis. However, IRS is associated with lower abundance than ITNs, with no An. gambiae s.s collected from window traps in IRS areas. This may account for the greater impact of IRS on prevalence. Similar results of IRS having a more prompt and powerful impact than ITNs on species abundance has been observed [32,33]. The apparent elimination of An. gambiae s.s in IRS areas and suppression of An. funestus s.s. and An. arabiensis to minimal levels, coupled with the absence of vector infectivity in both IRS and ITNs settings is striking. This effect of reducing abundance and infectivity of malaria vectors results in community wide protection [34,35].
In contrast to the current study, where no infected mosquitoes were collected, previous studies before and early into vector control in Zambia found P. falciparum sporozoites in all three major vectors (An. gambaie s.s., An. arabiensis and An. funestus s.s.) [36–38], although this may in part be a function of the different collection methods employed. The identification of An. nili and An. funestus-like species in Zambia, as well as the presence of An. rivulorum increases our knowledge of the range of these species. Further work to assess their transmission potential in Zambia is necessary. Indoor interventions have been shown to significantly reduce transmission in neighboring Tanzania. However, a residual transmission of disease remained through outdoor biting of An. gambiae s.l. [39]. This may account in part for the transmission that is still observed here, which will not have been detected in the methods used. This requires further investigation if the control programme is to reduce prevalence further.
The evidence of pyrethroid resistance spreading in Africa is mounting [20,40,41] and in some cases has resulted in policy changes in vector control interventions [20,41,42]. High levels of pyrethroid and DDT resistance were detected for the first time in both the An. gambiae s.s and An. funestus s.s in Zambia during this study, and these findings were subsequently confirmed by other groups. There was significant variation in the frequency of resistance detected between IRS and ITN localities, with higher levels of resistance (P<0.0001) being detected in IRS areas compared to ITNs areas.
Pyrethroid-DDT cross resistance from a common kdr mechanism, has been reported in An. gambiae s.s in Africa [21,40,43]. Samples of An. gambiae s.s that were pyrethroid and DDT resistant were tested for east and west kdr mutations. The west kdr mutation was found in all sites tested. This is the most southerly documented detection of this mutation. It is not yet clear whether this resistance has arisen de novo in Zambia or whether it has spread from other locations in Africa, but analysis of introns within the kdr gene would clarify the origin of the resistance. Anopheles funestus was also resistant to pyrethroids and DDT at a high frequency. To date there have been no reports of sodium channel mutations in An. funestus. The DDT and pyrethroid resistance detected here could arise from two separate metabolic resistance mechanisms, GST and P450 [44] respectively, or may be the first instance of kdr-type resistance in this species documented. Sequencing of the relevant areas of the sodium channel gene in resistant specimens of An. funestus is needed to clarify this. However it is notable that the pyrethroid resistant An. funestus found in Mozambique is not accompanied by resistance to DDT [41,45]. The detection of multiple resistance in both major vectors of malaria in Zambia may have grave implications for the malaria control programme. It may compromise the efficacy of interventions and potentially lead to the failure of IRS and ITNs based vector control. While this data serves to highlight the threats more information is required on all the underlying mechanisms of insecticide resistance so that a suitable insecticide resistance management plan may be put in place to sustain vector control.
Monitoring malaria cases in Kwa-Zulu Natal, South Africa, picked up the failure of pyrethroids in the IRS programme in the 1990s due to P450 mediated pyrethroid-resistance selection in An. funestus, resulting in DDT being successfully reintroduced [46], The resulting decline in malaria was reinforced by a change in drug treatment policy [47]. In Bioko Island, Equatorial Guinea, IRS with pyrethroids had a significant impact on the abundance of An. funestus but not on An. gambiae. However, a reduced sporozoite level in An. gambiae was observed that will have lowered transmission. With the detection of high frequencies of kdr in An. gambiae on the island a policy change to carbamate was made which appeared to significantly reduce the abundance of An. gambiae and maintained the low levels of An. funestus [20].
In 2008 the percentage of children aged 1–5 years positive for parasites fell from 25% to <15% in Zambia due to increased malaria control efforts (Chizema-Kawesha, 2010).The monitoring, evaluation and surveillance carried out over a three year period demonstrates the success of Zambia's control programme in reducing the burden of malaria further as signified by reduced prevalence of malarial infection to 6.8%. However, the recent detection of high insecticide resistance to pyrethroids and DDT in An. gambiae and An. funestus along with kdr detection in An. gambiae threatens the sustainability of this programme. While the lack of sporozoite positive mosquitoes in the window traps is consistent with reduced levels of transmission the rebound in prevalence of P. falciparum in IRS areas may be due to the increased levels of insecticide resistance and the beginning of control programme failure.
Acknowledgments
We would like to thank the Ministry of Health Zambia, and all the staff at the National Malaria Control Centre whose assistance was essential to this study. We would like to acknowledge John Miller (MACEPA) for all his assistance with the surveys in Zambia.
Author Contributions
Conceived and designed the experiments: MC IK EC-K. Performed the experiments: MC IK EC-K AMR VR FNP MK VM. Analyzed the data: MC IK JH EC-K AMR. Contributed reagents/materials/analysis tools: MC SC. Wrote the paper: EC-K IK JH MC. Managed the project in Zambia: EC KSB. Completed field collection: DM EC MC MK.
References
- 1. Feachem R, Sabot O (2008) A new global malaria eradication strategy. Lancet 371: 1633–1635.
- 2.
W.H.O (2009) World Malaria Report 2009.
- 3. Kleinschmidt I, Schwabe C, Shiva M, Segura JL, Sima V, et al. (2009) Combining indoor residual spraying and insecticide-treated net interventions. Am J Trop Med Hyg 81: 519–524.
- 4.
World Health Organization (2006) Pesticides and their Application.
- 5.
Ministry of Health (2000) Malaria Situation Analysis, National Malaria Control Programme, Lusaka, Zambia.
- 6. Ekue JM, Ulrich AM, Njelesani EK (1983) Plasmodium malaria resistant to chloroquine in a Zambian living in Zambia. Br Med J (Clin Res Ed) 286: 1315–1316.
- 7. Sharp B, van WP, Sikasote JB, Banda P, Kleinschmidt I (2002) Malaria control by residual insecticide spraying in Chingola and Chililabombwe, Copperbelt Province, Zambia. Trop Med Int Health 7: 732–736.
- 8.
Chanda E, Masaninga F, Coleman M, Sikaala C, Katebe C, et al. (2008) Integrated vector management: the Zambian experience. Malar J. 7. (164).164 p.
- 9. Chizema-Kawesha E, Miller JM, Steketee RW, Mukonka VM, Mukuka C, et al. (2010) Scaling up malaria control in Zambia: progress and impact 2005-2008. Am J Trop Med Hyg 83: 480–488.
- 10.
[Anon ymous] (2011) Innovative Vector Control Consortium: www.ivcc.com/projects/mdss.htm.
- 11. Curtis C (1994) The case for malaria control by genetic manipulation of its vectors. Parasitol Today 10: 371–374.
- 12.
Gillies MT, DeMeillon B (1968) The Anophelinae of Africa South of the Sahara. The South African Institute for Medical Research. 111111 p.
- 13.
Gillies MT, Coetzee M (1987) A supplement to: The Anophelinae of Africa South of the Sahara. The South African Institute for Medical Research.
- 14. Koekemoer LL, Kamau L, Hunt RH, Coetzee M (2002) A cocktail polymerase chain reaction assay to identify members of the Anopheles funestus (Diptera: Culicidae) group. Am J Trop Med Hyg 66: 804–811.
- 15. Scott JA, Brogdon WG, Collins FH (1993) Identification of single specimens of the Anopheles gambiae complex by the polymerase chain reaction. Am J Trop Med Hyg 49: 520–529.
- 16. Kengne P, Trung HD, Baimai V, Coosemans M, Manguin S (2001) A multiplex PCR-based method derived from random amplified polymorphic DNA (RAPD) markers for the identification of species of the Anopheles minimus group in Southeast Asia. Insect Mol Biol 2001 Oct 10(5): 427–35 10:427-435.
- 17. Spillings BL, Brooke BD, Koekemoer LL, Chiphwanya J, Coetzee M, et al. (2009) A new species concealed by Anopheles funestus Giles, a major malaria vector in Africa. Am J Trop Med Hyg 81: 510–515.
- 18. Favia G, Della TA, Bagayoko M, Lanfrancotti A, Sagnon N, et al. (1997) Molecular identification of sympatric chromosomal forms of Anopheles gambiae and further evidence of their reproductive isolation. Insect Mol Biol 6: 377–383.
- 19. Bass C, Nikou D, Blagborough AM, Vontas J, Sinden RE, et al. (2008) PCR-based detection of Plasmodium in Anopheles mosquitoes: a comparison of a new high-throughput assay with existing methods. Malar J 7(177.): 177.
- 20. Sharp BL, Ridl FC, Govender D, Kuklinski J, Kleinschmidt I (2007) Malaria vector control by indoor residual insecticide spraying on the tropical island of Bioko, Equatorial Guinea. Malar J 6(52.): 52.
- 21. Martinez-Torres D, Chandre F, Williamson MS, Darriet F, Berge JB, et al. (1998) Molecular characterization of pyrethroid knockdown resistance (kdr) in the major malaria vector Anopheles gambiae s.s. Insect Mol Biol 7: 179–184.
- 22. Janeira F, Vicente JL, Kanganje Y, Moreno M, Do Rosario VE, et al. (2008) A primer-introduced restriction analysis-polymerase chain reaction method to detect knockdown resistance mutations in Anopheles gambiae. J Med Entomol 45: 237–241.
- 23. Bass C, Nikou D, Donnelly MJ, Williamson MS, Ranson H, et al. (2007) Detection of knockdown resistance (kdr) mutations in Anopheles gambiae: a comparison of two new high-throughput assays with existing methods. Malar J 6(111.): 111.
- 24. W.H.O (1998) Test Procedures for Insecticide Resistance Monitoring in Malaria Vectors, Bio-Efficacy and Persistence of Insecticides on Treated Surfaces. Report of the WHO informal Consultation, 28-30 September WHO/CDS/CPC/MAL/98.12.
- 25. Fegan GW, Noor AM, Akhwale WS, Cousens S, Snow RW (2007) Effect of expanded insecticide-treated bednet coverage on child survival in rural Kenya: a longitudinal study. Lancet 370: 1035–1039.
- 26. Komatsu R, Low-Beer D, Schwartlander B (2007) Global Fund-supported programmes contribution to international targets and the Millennium Development Goals: an initial analysis. Bull World Health Organ 85: 805–811.
- 27. Beier JC, Killeen GF, Githure JI (1999) Short report: entomologic inoculation rates and Plasmodium falciparum malaria prevalence in Africa. Am J Trop Med Hyg 61: 109–113.
- 28. McClean KL, Senthilselvan A (2002) Mosquito bed nets: implementation in rural villages in Zambia and the effect on subclinical parasitaemia and haemoglobin. Trop Doct 32: 139–142.
- 29. Chanda P, Hamainza B, Mulenga S, Chalwe V, Msiska C, et al. (2009) Early results of integrated malaria control and implications for the management of fever in under-five children at a peripheral health facility: a case study of Chongwe rural health centre in Zambia. Malar J 8(49.): 49.
- 30. Killeen GF, McKenzie FE, Foy BD, Schieffelin C, Billingsley PF, et al. (2000) A simplified model for predicting malaria entomologic inoculation rates based on entomologic and parasitologic parameters relevant to control. Am J Trop Med Hyg 62: 535–544.
- 31. Protopopoff N, Van BW, Marcotty T, Van HM, Maes P, et al. (2007) Spatial targeted vector control in the highlands of Burundi and its impact on malaria transmission. Malar J 6(158.): 158.
- 32. Roberts JM (1964) The Control of Epidemic Malaria in the Highlands of Western Kenya. II. The Campaign. J Trop Med Hyg 67: 191–9 CONTD.:191-199.
- 33. Guyatt HL, Kinnear J, Burini M, Snow RW (2002) A comparative cost analysis of insecticide-treated nets and indoor residual spraying in highland Kenya. Health Policy Plan 17: 144–153.
- 34. Lengeler C (2004) Insecticide-treated nets for malaria control: real gains. Bull World Health Organ 82: 84.
- 35. Lengeler C (2000) Insecticide-treated bednets and curtains for preventing malaria. Cochrane Database Syst Rev CD000363:
- 36. Kent RJ, Thuma PE, Mharakurwa S, Norris DE (2007) Seasonality, blood feeding behavior, and transmission of Plasmodium falciparum by Anopheles arabiensis after an extended drought in southern Zambia. Am J Trop Med Hyg 76: 267–274.
- 37. Shelly AJ (1973) Observations on the behaviour of Anopheles gambiae sp. B in the Kambole village in the Zambezi valley, Zambia. Ann Trop Med Hyg 67: 237–248.
- 38. Bransby-Williams WR (1979) House catches of adult Anopheles gambiae species B in two areas of Zambia. East Afr Med J 56: 557–561.
- 39. Russell TL, Govella NJ, Azizi S, Drakeley CJ, Kachur SP, et al. (2011) Increased proportions of outdoor feeding among residual malaria vector populations following increased use of insecticide-treated nets in rural Tanzania. Malar J 10(80.): 80.
- 40. Coleman M, Sharp B, Seocharan I, Hemingway J (2006) Developing an evidence-based decision support system for rational insecticide choice in the control of African malaria vectors. J Med Entomol 43: 663–668.
- 41. Casimiro SL, Hemingway J, Sharp BL, Coleman M (2007) Monitoring the operational impact of insecticide usage for malaria control on Anopheles funestus from Mozambique. Malar J 6: 142.
- 42. N'Guessan R, Corbel V, Akogbeto M, Rowland M (2007) Reduced efficacy of insecticide-treated nets and indoor residual spraying for malaria control in pyrethroid resistance area, Benin. Emerg Infect Dis 13: 199–206.
- 43. Ranson H, Jensen B, Vulule JM, Wang X, Hemingway J, et al. (2000) Identification of a point mutation in the voltage-gated sodium channel gene of Kenyan Anopheles gambiae associated with resistance to DDT and pyrethroids. Insect Mol Biol 9: 491–497.
- 44. Hemingway J, Hawkes NJ, McCarroll L, Ranson H (2004) The molecular basis of insecticide resistance in mosquitoes. Insect Biochem Mol Biol 34: 653–665.
- 45. Cuamba N, Morgan JC, Irving H, Steven A, Wondji CS (2010) High level of pyrethroid resistance in an Anopheles funestus population of the Chokwe District in Mozambique. PLoS One 5: e11010.
- 46. Maharaj R, Mthembu DJ, Sharp BL (2005) Impact of DDT re-introduction on malaria transmission in KwaZulu-Natal. S Afr Med J 95: 871–874.
- 47. Barnes KI, Durrheim DN, Little F, Jackson A, Mehta U, et al. (2005) Effect of Artemether-Lumefantrine Policy and Improved Vector Control on Malaria Burden in KwaZulu-Natal, South Africa. PLoS Med 2: e330.