https://orcid.org/0000-0001-9913-8883
Figures
Abstract
Background & aims
Progranulin (PGRN) is known to promote tumorigenesis and proliferation of several types of cancer cells. However, little is known about the clinicopathological features of patients with gastrointestinal stromal tumors (GISTs) with regard to PGRN expression.
Methods
A retrospective analysis was performed on patients with GISTs who underwent curative surgical resection between 2007 and 2017. PGRN expression was evaluated by immunohistochemical (IHC) analysis and semi-quantitatively categorized (no expression, 0; weak, 1+; moderate, 2+; strong, 3+). Tumors with a staining intensity of 2+ or 3+ were considered high PGRN expression.
Results
Fifty-four patients were analyzed; 31 patients (57%) were male. The median age at surgery was 60 years (range, 33–79), and the most common primary site was the stomach (67%). Thirty-five patients (65%) had spindle histology; 42 patients (78%) were separated as a high-risk group according to the modified National Institutes of Health (NIH) classification. High PGRN-expressing tumors were observed in 27 patients (50%), had more epithelioid/mixed histology (68% vs. 32%; p = 0.046), and KIT exon 11 mutations (76% vs. 24%; p = 0.037). Patients with high PGRN-expressing tumors had a worse recurrence-free survival (RFS) (36% of 5-year RFS) compared to those with low PGRN-expressing tumors (96%; p<0.001). Multivariate analysis showed that high PGRN expression and old age (>60 years) were independent prognostic factors for poor RFS.
Citation: Do I-G, Jung KU, Koo D-H, Lee Y-G, Oh S, Kim K, et al. (2021) Clinicopathological characteristics and outcomes of gastrointestinal stromal tumors with high progranulin expression. PLoS ONE 16(1): e0245153. https://doi.org/10.1371/journal.pone.0245153
Editor: Filomena de Nigris, Universita degli Studi della Campania Luigi Vanvitelli, ITALY
Received: July 16, 2020; Accepted: December 22, 2020; Published: January 7, 2021
Copyright: © 2021 Do et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
Data Availability: Thank you for your understanding that data sharing is limited because this study is a study involving patients who have undergone surgery for a small number of rare diseases and contains sensitive data that participants can be identified. To request a minimum data set for this study, please contact Kangbuk Samsung Hospital Institutional Review Board (IRB, Tel. +82-2001-1943; e-mail: irb.kbsmc@samsung.com). Then, we will share the anonymized data after obtaining approval from this IRB.
Funding: The authors received no specific funding for this work.
Competing interests: The authors have read the journal’s policy and have the following competing interests: GS is a paid employee of A&G Pharmaceutical, Inc. There are no patents, products in development or marketed products associated with this research to declare. This does not alter our adherence to PLOS ONE policies on sharing data and materials.
Introduction
Gastrointestinal stromal tumors (GISTs) are rare tumors, but the most common form of mesenchymal tumors that originate from the interstitial cells of Cajal in the gastrointestinal wall [1]. GISTs are characterized by primary activating mutations in KIT or platelet-derived growth factor alpha (PDGFRA) genes that result in constitutive activation of receptor tyrosine kinase activity [2]. The standard treatment for localized GISTs is macroscopically complete surgical resection. Since the introduction of molecular-targeted therapy, such as imatinib, the outcomes of advanced GISTs have remarkably improved [3].
Recently, the mutations in the progranulin (PGRN) gene has been known to be related with the onset of neurodegenerative diseases [4]. While, in the oncology field, the association between PGRN and several malignancies such as leukemia, breast cancer, thyroid cancer, or ovary cancer has been discovered [5]. PGRN is a secreted glycoprotein recognized as an adipokine involved in diet-induced obesity, insulin resistance, or tumor progression cascade, including proliferation, migration, and angiogenesis [6, 7]. Recently, upregulation of PGRN in colorectal cancer has been shown to be correlated with tumor proliferation and vascular endothelial growth factor A (VEGF-A) expression, as well as increased microvessel density [8]. These increased Ki-67 and VEGF-A expressions, which were mediated by both tumor necrosis factor receptor-2 (TNFR2)/protein kinase B (Akt) and extracellular signal-regulated kinase (ERK) signaling pathways, suggested that PGRN might represent a kind of new growth factors in gastrointestinal cancer.
However, little is currently known about PGRN expression in GISTs with regard to clinicopathological features or the prognostic role of PGRN overexpression in patients with GISTs who have undergone curative resection. Therefore, this study evaluated PGRN expression in patients with GISTs who underwent curative resection and determined its prognostic role.
Materials and methods
Patients
We retrospectively reviewed the medical records of patients with GISTs who underwent surgery at Kangbuk Samsung Hospital (Seoul, Korea) between March 2007 and March 2017, and evaluated their tumor tissues. Patients who met the following criteria were included in our analysis: ≥18 years old; histologically confirmed GIST; curative-intent surgery without residual lesions; no history of other malignancies; and tumor tissue available for immunohistochemical (IHC) examination of PGRN expression. The study was approved by the Institutional Review Board (IRB) of Kangbuk Samsung Hospital (KBSMC 2019-07-014). Our analysis was a retrospective design using fully anonymized data, so the IRB waived the requirement for informed consent.
Immunohistochemical analysis
Specimens from formalin-fixed and paraffin-embedded samples at the time of surgery were collected for histological review and IHC analysis. IHC was performed using a Bond-max Autoimmunostainer (Leica Biosystems, Melbourne, Australia) with BondTM Polymer Refine Detection, DS9800 (Vision Biosystems, Melbourne, Australia) as described previously [9]. Briefly, formalin-fixed, paraffin-embedded, 4-μm-thick tissue sections were deparaffinized three times in xylene for a total of 15 min and subsequently rehydrated. Antigen retrieval was carried out at 97°C for 20 min in ER1 buffer. After blocking endogenous peroxidase activity with 3% hydrogen peroxidase for 10 min, slides were incubated with mouse monoclonal anti-progranulin antibody (PG359-7) (AG-20A-0052, Adipogen Life Sciences, Liestal, Switzerland) at a dilution of 1:500 for 15 min at room temperature. Negative controls (substitution of the primary antibody with Tris-buffered saline) were run simultaneously. The slides were assessed without knowledge of the clinical outcome. Progranulin was expressed in the cytoplasm of tumor or peritumoral cells with a granular pattern. PGRN expression in tumor cells was semi-quantitatively categorized, as previously described (no expression, 0; weak/focal, 1+; moderate/focal or diffuse, 2+; strong/diffuse, 3+) [10]. Tumor cells with ≥2+ PGRN staining were considered to be high PGRN-expressing tumors, while tumors with ≤1+ staining were considered to be low PGRN-expressing tumors (Fig 1).
Progranulin expression in tumor cells by immunohistochemical staining (×200) (A) hematoxylin and eosin stain; (B) intensity 1+; (C) intensity 2+; (D) intensity 3+.
Statistical analysis
Associations between PGRN expression and clinical characteristics were assessed by univariate chi-square analysis using a dichotomized cut-off value for IHC positivity. Recurrence-free survival (RFS) was calculated as the duration from the date of surgery to the date of documented recurrence, death, or last follow-up (whichever occurred first). OS time was calculated as the duration from the date of surgery to the date of death or last follow-up. Survival curves were constructed using the Kaplan–Meier method and were compared using the log-rank test. Multivariate analysis was performed using the Cox proportional hazard regression model with the enter method. A two-sided P-value<0.05 was considered significant, and 95% confidence intervals (CIs) were calculated. All statistical analyses were performed using IBM SPSS ver. 25.0 (IBM Co., NY, USA).
Results
Baseline characteristics
A total of 54 patients were evaluated and their clinical characteristics are summarized in Table 1. Thirty-one patients (57%) were male and the median age at the time of surgery was 60 years (range 33–79). Nine patients (17%) had diabetes mellitus, and the mean body weight and body mass index (BMI) was 62.2 kg (± standard deviation [SD], 8.7) and 23.7 m2 (± SD, 2.7), respectively. Forty-four patients (82%) had localized resectable disease, and 10 patients (19%) had resectable metastatic disease. The most common primary site was the stomach (67%), followed by the small bowel (26%). The common GIST histology was spindle cell type (65%), and the median tumor size was 8 cm (range, 2–47). Using the modified National Institutes of Health (NIH) classification and according to the size and mitosis index of primary tumors, the high-risk group was the most common (80%). Among the 34 patients who had genotyping performed, the KIT exon 11 mutation was the most common (74%), and the PDGFRA exon 8 D842V mutation was observed in 3 patients (9%). The pathologic characteristics are summarized in Table 2 and Fig 2.
Clinicopathological features according to PGRN expression
Twenty-seven patients (50%) had high PGRN-expressing tumors with a staining intensity of 2+ or 3+. There was no significant difference in clinicopathologic factors, including sex, age, diabetes, BMI, cholesterol, primary site, size, mitosis and immunohistochemical staining status between patients with high PGRN-expressing and low PGRN-expressing GISTs (Table 3). However, the patients with high PGRN-expressing tumors showed more epithelioid or mixed histology (48% vs. 22%; P = 0.046) and KIT exon 11 mutations (91% vs. 46%; P = 0.037) than those with low PGRN-expressing tumors.
Survival outcomes according to PGRN expression
The median follow-up duration of 50 living patients was 52.8 months (interquartile range [IQR], 27.7–73.5). Most death events were observed in metastatic tumors (N = 3, 75%) and in high PGRN-expressing tumors (N = 3, 75%). According to tumor status, the 5-year RFS of non-metastatic and metastatic patients was 70.0% (95% CI 56.5–83.5) and 35.6% (95% CI 6.0–65.3), respectively (P = 0.023; Fig 3). In the patients with non-metastatic GISTs, the 5-year RFS of low-risk, intermediate-risk, and high-risk patients according to the modified NIH classification was 100.0%, 53.3% (95% CI 10.0–97.0), and 66.1% (95% CI 50.0–82.3), respectively (P = 0.242). Patients with high PGRN-expressing tumors had a worse 5-year RFS (35.6%, 95% CI 15.1–56.1) compared to those with low PGRN-expressing tumors (95.7%, 95% CI 87.4–100.0, P<0.001; Table 4). Furthermore, the non-metastatic patients were categorized into five groups by combining PGRN expression and the modified NIH classification; the 5-year RFS was evaluated as follows: 1) low risk (regardless of PGRN) = 100.0%; 2) intermediate risk with low PGRN = 100.0%; 3) intermediate risk with high PGRN = 37.5% (95% CI 0.0–84.9); 4) high risk with low PGRN = 94.4% (95% CI 83.8–100.0); and 5) high risk with high PGRN = 21.3% (95% CI 1.0–42.0, P<0.001). In the patients with metastatic GISTs, there was a trend of poor RFS in the high PGRN-expressing tumors (median, 36.8 months, 95% CI 8.6–65.0) compared to the low PGRN-expressing tumors (70.7 months, 95% CI not evaluable; P = 0.104). The 5-year OS of non-metastatic and metastatic patients was 93.8% (95% CI 86.7–100.0) and 75.0% (95% CI 48.2–100.0), respectively (P = 0.021). Multivariate analysis in non-metastatic disease showed that high PGRN expression (hazard ratio [HR] 51.4, 95% CI 3.2–821.9, P = 0.005) and old age (>60 years; HR 5.7, 95% CI 1.01–31.7, P = 0.049) were independent prognostic factors associated with poor RFS (Table 4).
Recurrence or progression-free survival (A) and overall survival (B) according to initial status; recurrence-free survival (C) in non-metastatic gastrointestinal stromal tumors (GISTs) and progression-free survival (D) in metastatic GISTs according to progranulin (PGRN) expression; recurrence-free survival in non-metastatic GISTs according to modified National Institutes of Health (NIH) classification (E) and modified NIH/PGRN expression (F).
Discussion
This study investigated the PGRN expression in resected GISTs and evaluated the clinicopathologic features of patients with GISTs according to PGRN expression. High PGRN-expressing tumors showed more epithelioid type histology and exon 11 mutation tumors. The RFS was worse in patients with GISTs with high PGRN expression regardless of the modified NIH risk assessment.
Clinicopathological features of PGRN-expressing GISTs
In several tumors, the relationships between PGRN expression and prognosis have been reported. Recently, PGRN has been evaluated as an adipose tissue hormone (adipokine) implicated in obesity and insulin resistance [11]. Particularly, PGRN indicated a poor prognosis in tumors mostly associated with metabolic syndrome such as breast cancer [10, 12], colorectal cancer [13], gallbladder cancer [9], bladder cancer [14], and prostate cancer [15]. In practice, patients with GISTs are usually diagnosed without symptoms, even in the advanced stage. Therefore, we evaluated the association between PGRN expression and metabolic parameters, but no association was observed with diabetes mellitus, body weight, BMI, cholesterol level, and fasting glucose levels. It is noteworthy, however, that high PGRN-expressing tumors show increased epithelioid/mixed type histology and KIT exon 11 mutations because several previous studies have shown that non-spindle cell histology and exon 11 mutations are associated with poor prognosis for GIST [16–18].
Clinical impact of PGRN-expressing GISTs
In multivariate analysis for RFS, old age and PGRN expression were found to be significant predictors of poor prognosis. As mentioned above, there was no significant difference in RFS according to risk classification, including tumor size and mitosis index. However, when PGRN expression and risk group classification were combined, intermediate-risk patients with high PGRN-expressing tumors, for whom postoperative imatinib was not recommended, showed worse prognosis than high-risk patients with low PGRN-expressing tumors, although imatinib was administered to some patients. This suggested that PGRN overexpression was significantly associated with poor RFS in patients with GISTs who had undergone curative resection. Furthermore, there was a trend of poor RFS in high PGRN-expressing tumors among the patients with resectable metastatic GISTs. Currently, however, there is limited understanding of the role of PGRN in GISTs. PGRN is known to promote proliferation and angiogenesis through TNFR2/Akt and ERK signaling pathways in other gastrointestinal tumors [8], and further investigation is needed to discover the role of PGRN as a biomarker and therapeutic target in GISTs.
Postoperative imatinib therapy during study periods
Recently, postoperative treatment has been standardized as a 3-year use of the adjuvant imatinib in patients with high-risk GISTs, based on a large phase III trial in which imatinib use for 36 months reduced the risk of recurrence and improved overall survival (OS) compared with imatinib use for 12 months in patients with GISTs with a high risk of recurrence after surgery [4, 5]. In this study, 43 patients were at high risk after surgery, and 25 (58%) received postoperative adjuvant imatinib therapy. Because of the introduction of postoperative imatinib therapy, it seems that there was no significant difference in RFS in the high-risk group and low- or intermediate-risk group according to the modified NIH risk. In Korea, the adjuvant imatinib therapy for 1 year was reimbursed from 2010, and adjuvant treatment for 3 years could become a standard therapy since the reimbursement eventually expanded to 3 years in November 2013. Imatinib was heterogeneously administered because this study included patients who had undergone surgical treatment for 11 years from 2007 to 2017.
Limitations and conclusions
Although the PGRN expression status was a statistically significant factor in terms of prediction of poor RFS, the results should be interpreted with caution. This study might be biased by the small size of cohort and retrospective design in a single institution; therefore, further studies are needed to confirm our results.
In conclusion, high PGRN-expressing GISTs had more epithelioid/mixed histology and KIT exon 11 mutations. Patients with GISTs expressing high levels of PGRN had significantly poorer outcomes than patients whose tumors expressed low levels of PGRN. Therefore, PGRN expression status may be a potential prognostic factor for patients with GISTs who have undergone curative resection.
Acknowledgments
This study was presented in part at the general poster session, Gastrointestinal Cancers Symposium, 2019 January 17–19, San Francisco, CA.
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