Chest
Original ResearchPulmonary HypertensionIncreased Expression of CD16, CD69, and Very Late Antigen-1 on Blood Monocytes in Active Sarcoidosis
Section snippets
Subjects
Twenty-three consecutive patients presenting to our department because of symptomatic sarcoidosis and ≥ 15% lymphocytes in BAL, a criterion for active pulmonary alveolitis, were included in this study. Table 1 summarizes the clinical characteristics of the patient group. The diagnosis of sarcoidosis was established on the basis of clinical findings and histologic evidence of noncaseating epithelioid granulomas and after exclusion of other known causes of granulomatosis in accordance with the
Flow Cytometric Analysis of Peripheral Blood Monocytes
Analysis of CD14+ monocytes gated within CD45+ leukocytes revealed no significant differences between groups in percentages of monocytes (Table 2). Notably, the side scatter pattern of the monocytes differed between patients and control subjects. The monocyte populations from patients showed an increased and more diverse side-light scatter characteristics (SSC), compatible with a granular appearance and an activated state (Fig 1 in online supplemental). Although no difference in frequencies of
Discussion
Activation of monocyte/alveolar macrophage cell lineage is thought to play an important role in the pathogenesis of sarcoidosis. The present study showed activated peripheral blood monocytes in patients with active pulmonary sarcoidosis, characterized by elevated expression of CD14, CD16, CD69, and VLA-1. The correlations observed between the cells expressing these markers strongly suggest that these cells are proinflammatory monocytes.
Our data in survey suggest that alteration of disease
Acknowledgment
The authors thank Mrs. A. van Heugten-Roeling and Mrs. C. Benschop (Department of Medical Microbiology and Immunology, St. Antonius Hospital) for expert technical assistance.
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2015, BloodCitation Excerpt :Also, all monocyte subsets, including the slan-positive nonclassical monocytes, are clearly separated from the CD1c+ DCs, which establishes that the slan-positive nonclassical monocytes are distinct from DCs. We then analyzed monocyte subsets based on slan and CD16 expression in sarcoidosis, a systemic granulomatous, inflammatory disease in which an increase in CD16-positive monocytes had been shown.8,16 We studied our patients before diagnostic bronchoscopy and before any immunosuppressive therapy with glucocorticoids.
The authors have no conflicts of interest to disclose.
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