Laser Capture Microdissection

1. Front Matter

   Pages i-xiv

   PDF

2. Introduction

   1. Front Matter

      Pages 1-1

      PDF

   2. An Introduction to Laser-Based Tissue Microdissection Techniques

      • Stephanie Curran, Graeme I. Murray

      Pages 3-7

3. Microdissection and DNA Analysis

   1. Front Matter

      Pages 9-9

      PDF

   2. Methacarn Fixation for Genomic DNA Analysis in Microdissected Cells

      • Makoto Shibutani, Chikako Uneyama

      Pages 11-26

   3. Multiplex Quantitative Real-Time PCR of Laser Microdissected Tissue

      • H. Patrick

      Pages 27-38

   4. comparative Genomic Hybridization Using DNA From Laser Capture Microdissected Tissue

      • Grace Callagy, Lucy Jackson, Carlos Caldas

      Pages 40-56

   5. Detection of Ki-ras and p53 Mutations by Laser Capture Microdissection/PCR/SSCP

      • Deborah Dillon, Karl Zheng, Brina Negin, José Costa

      Pages 57-67

   6. Whole-Genome Allelotyping Using Laser Microdissected Tissue

      • Colleen M. Feltmate, Samuel C. Mok

      Pages 69-78

   7. Microdissection for Detecting Genetic Aberrations in Early and Advanced Human Urinary Bladder Cancer

      • Arndt Hartmann, Robert Stoehr, Peter J. Wild, Wolfgang Dietmaier, Ruth Knuechel

      Pages 79-92

   8. Laser Microdissection for Microsatellite Analysis in Colon and Breast Cancer

      • Peter J. Wild, Robert Stoehr, Ruth Knuechel, Arndt Hartmann, Wolfgang Dietmaier

      Pages 93-102

   9. Assessment of RET/PTC Oncogene Activation in Thyroid Nodules Utilizing Laser Microdissection Followed by Nested RT-PCR

      • Giovanni Tallini, Guilherme Brandao

      Pages 103-112

   10. Combined Laser-Assisted Microdissection and Short Tandem Repeat Analysis for Detection of In Situ Microchimerism After Solid Organ Transplantation Ulrich Lehmann, Anne Versmold, and Hans Kreipe

       • Makoto Shibutani, Chikako Uneyama

       Pages 113-124

4. RNA and Gene Expression Studies Using Microdissected Cells

   1. Front Matter

      Pages 125-125

      PDF

   2. Laser-Assisted Microdissection of Membrane-Mounted Tissue Sections

      • Lise Mette, Stephen Hamilton-Dutoit

      Pages 127-138

   3. Laser-Assisted Microdissection of Membrane-Mounted Sections Following Immunohistochemistry and In Situ Hybridization

      • Lise Mette Gjerdrum, Stephen Hamilton-Dutoit

      Pages 139-150

   4. Laser-Assisted Cell Microdissection Using the PALM System

      • Patrick Micke, Arne Östman, Joakim Lundeberg, Fredrik Ponten

      Pages 151-166

   5. Laser Microdissection and RNA Analysis

      • Ludger Fink, Rainer Maria Bohle

      Pages 167-185

   6. Gene Expression Profiling of Primary Tumor Cell Populations Using Laser Capture Microdissection, RNA Transcript Amplification, and GeneChip® Microarrays

      • Veronica I. Luzzi, Victoria Holtschlag, Mark A. Watson

      Pages 187-208

   7. 16 Quantification of Gene Expression in Mouse and Human Renal Proximal Tubules

      • Jun-ya Kaimori, Masaru Takenaka, Kousaku Okubo

      Pages 209-220

Expert laboratorians noted for their optimized methods describe in step-by-step detail how to successfully carry out tissue-based laser microdissection in their own laboratory using the different systems available and to apply a wide range of sophisticated molecular technologies to the microdissected tissue samples. Highlights include gene expression profiling of primary tumor cell populations using laser capture microdissection, RNA transcript amplification and GeneChip® microarrays, laser capture microdissection and colorectal cancer proteomics, and proteomic analysis of human bladder tissue using SELDI® approach following microdissection techniques. Applications of the microdissected tissue described encompass PCR, gene expression studies including microarray analysis, and proteomics.

• DNA
• Microarray
• PCR
• Proteomics
• gene expression
• genes
• hybridization
• microorganisms

From the reviews:

"...an excellent help to researchers...." - Neoplasma

"Comprehensive and state of the art, this book provides a highly practical guide to successful tissue-based laser microdissection using available commercial systems, and also a guide to interpreting the results using a wide range of sophisticated molecular technologies." - Clinical Laboratory International

"...provides researchers with practical advise and detailed technical protocols for carrying out tissue-based LCM...." - Vet Pathol

"Comprehensive and up-to-date collection of readily reproducible preparative and analytical methods is presented in each chapter for an in-depth practice of laser microdissection application. … The book is subdivided into 5 parts with a total of 24 chapters featuring step-by-step protocols that will enable novice researchers to isolate specific cell types or microorganisms." (Carlo Alberto Redi, European Journal of Histochemistry, December, 2005)

• Department of Pathology, University of Aberdeen, Aberdeen, UK

  Graeme I. Murray, Stephanie Curran

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