Abstract
The importance of interactive effects, of minerals in general, on nutrient requirements is becoming increasingly recognized. The interaction between iron and zinc has not been as widely investigated. The metabolic interrelationships between dietary iron and zinc have been known for years, but some subtle relationships may have gone unrecognized. Because nutrient interactions are not necessarily linear in nature, it may be inadequate to apply linear statistical models to study the interaction between zinc and iron. In this study, we used traditional as well as a nonlinear approach in analyzing experimental results from groups of rats fed a wide range of dietary zinc and iron. Male weanling Sprague-Dawley rats were used in a 5 × 4 factorially arranged experiment. Dietary variables were iron (as ferric citrate) at 4, 12, 24, 48, or 96 µg Fe/g diet and zinc (as zinc carbonate) at 5, 10, 20, or 40 µg Zn/g diet. After 7 wk, hematological parameters were measured and plasma ceruloplasmin and cholesterol were determined. In addition to interactive effects as shown by analysis of variance, the application of log-linear analysis to the experimental data revealed a far broader range of interactions between dietary iron and zinc. As a result of our experiment and its quantitative analysis, we conclude that the interaction between iron and zinc is nutritionally important and that dietary iron affected the response of many blood parameters to dietary zinc. The complete dataset can be found at http://www.gfhnrc.ars.usda.gov/fezn.
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U.S. Department of Agriculture, Agricultural Research Service, Northern Plains Area is an equal opportunity/affirmative action employer and all agency services are available without discrimination.
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Uthus, E.O., Zaslavsky, B. Interaction between zinc and iron in rats. Biol Trace Elem Res 82, 167–183 (2001). https://doi.org/10.1385/BTER:82:1-3:167
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DOI: https://doi.org/10.1385/BTER:82:1-3:167