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MALDI-TOF mass spectrometry

A versatile tool for high-performance DNA analysis

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Abstract

Matrix-assisted laser desorption/ionization (MALDI) time-of-flight (TOF) mass spectrometry (MS) has developed during the past decade into a versatile tool for biopolymer analysis. The aim of this review is to summarize this development and outline the applications, which have been enabled for routine use in the field of nucleic acid analysis. These include the anlaysis of mutations, the resequencing of amplicons with a known reference sequence, and the quantitative analysis of gene expression and allelic frequencies in complex DNA mixtures.

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  1. Sequenom, Inc., Johns Hopkins Court, 92121, San Diego, CA

    Christian Jurinke

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Jurinke, C., Oeth, P. & van den Boom, D. MALDI-TOF mass spectrometry. Mol Biotechnol 26, 147–163 (2004). https://doi.org/10.1385/MB:26:2:147

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