Abstract
Calcium phosphate ceramics are bioactive, osteoconductive materials used as guides for bone regeneration. We were able to carry out a histological study of ceramics implanted in large mammals and humans. An identical sequence of events apparently occurred at the contact of the ceramics. The osteoblasts differentiated preferentially in close proximity to the ceramic surface in animals. The early stage lasted longer in humans and was characterized by the presence of a monolayer of multinucleated cells containing TRAP+ (tartrate resistant acid phosphatase activity) granules at the surface of the ceramic. The loose connective tissue invading the pores contained almost exclusively fibroblast-like cells with TRAP+ nucleus. Differentiation of the osteogenic cells around the ceramic is diffuse in humans and not always related to the material surface. Ceramics become embedded in immature bone both in humans and animals. The immature bone and the ceramics are then remodeled and progressively replaced by layered bone. The degradation of the ceramic occurs preferentially at the grain boundaries and the grains released are phagocytosed by the macrophages when below a critical size and degraded in the low pH compartments of the cell. In conclusion: the integration of calcium phosphate ceramics is comparable in animals and man. Although in the cases studied the ceramics were unable to induce osteogenic differentiation, they did favor the differentiation of pre-determined osteogenic cells.
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References
F. Cabanne, J.L. Bonenfant, Inflammation in Anatomie Pathologique. Principes de Pathologie Générale et Spéciale. (Presses de l’Université Laval, Québec, Maloine, SA Editeur, Paris. 1980) p 115.
M. Naito, Archiv. Histol. Cytol. 56 (4), 331–351 (1993).
Y. Takahashi, M. Naito, and M. Takeya., Pathology International 46, 473–485 (1996).
S.B. Goodman, P. Huie, Y. Song, K. Lee, A. Doshi, B. Rushdieh, S. Woolson, W. Maloney, D. Schurman, and R. Sibley, Clin Orthop Rel Res 337, 149–163 (1997).
Y. Kadoya, A. Kobayashi, and H. Ohashi, Acta Orthop Scand 69 (supplementum 278)1–16 (1998).
N. A. Athanasou, J Bone Joint Surg 78 (A), 7–1090 (1996).
P. Frayssinet, J.L. Trouillet, N. Rouquet, E. Azimus, A. Biomaterials, 14 (6), 423–429 (1993).
K. D. Johnson, K. E. Frierson, T. S. Keller, C. Cook, R. Scheinberg, J. Zerwekh, L. Meyers, and M. F. Sciadini. J Orthop Res 14, 351–369 (1996).
L.L. Hench, J.W. Wilson, Sciences, 226, 630–668 (1984)
M. Heughebaert, R. Z. LeGeros, M. Gineste, and A. Guilhem, J Biomed Mat Res 22, 257–268 (1988).
G. Daculsi, R. Z. LeGeros, M. Heughebaert, and I. Barbieux, Calcif Tissue Int 46, 20–27 (1990).
A. El-Ghannam, P. Ducheyne, and I. M. Shapiro. Biomaterials 18, 295–303 (1997).
J. E. Davies, The Anatomical Record 245, 426–445 (1996).
P. Frayssinet, C. Delga, P. Conte, N. Rouquet, M. Nadal, In: Proceedings of the Electron Microscopy Society of America. Fiftieth Annual Meeting 1992, edited by G.W. Bailey, J. Bentley, and J.A. Small (San Francisco Press, Inc., San Francisco 1992), pp 784–785.
J.D. de Bruijn, Thesis Leiden, (1993)
S.P. Bruder, A.I. Caplan, Bone 11, 133–139 (1990)
C. Klein, K. de Groot, W. Chen, Y. Li, X. Zhang, Biomaterials 15, 31–34 (1994).
S.P.Van Eeden, U. Ripamonti, Plast Reconstr Surg 93, 959–966 (1994).
H. Yamasaky, H. Sakai, Biomaterials 13, 308–312 (1992).
Z. Yang, H. Yuan, W. Tong, P. Zou, W. Chen, X. Zhang, Biomaterials 17, 2131–2137 (1996).
P. Frayssinet, N. Rouquet, F. Tourenne, J. Fages, G. Bonel, Cells and materials 4, 383–394 (1994).
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Frayssinet, P., Schwartz, C., Mathon, D. et al. Osteointegration of Calcium Phosphate Ceramics in Humans and Animals. MRS Online Proceedings Library 599, 3–13 (1999). https://doi.org/10.1557/PROC-599-3
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DOI: https://doi.org/10.1557/PROC-599-3