[Purpose] This meta-analysis was undertaken to establish normative reference values for the two-minute walk test. [Methods] Three database searches and a hand search were conducted. Meta-analysis was used to consolidate two-minute walk test data stratified by gender and age group. [Results] Data from four articles was consolidated. Normative two-minute walk test distances varied from 150.3 m (women, 70 to 79 years) to 217.9 m (men, 20 to 29 tears). [Conclusion] Normative reference values derived using meta-analysis should provide a better standard than individual studies for interpreting the two-minute walk test performance of adults.