[Purpose] The purpose of this study was to investigate the preventive effects of stretching and stabilization exercises on muscle fatigue of the neck erector spine and upper trapezius muscles. [Subjects and Methods] The subjects of this study were 26 healthy university students (14 males and 12 females). Each subject was assigned randomly to each of three study groups in order to prevent order effect. The three groups included in this study were the no-exercise, stretching exercise, and neck stabilization exercise groups. The median electromyographic frequency was used as a gauge of muscle fatigue. [Results] Decreased muscle fatigue was demonstrated by an increase in the median electromyographic frequency values in all the four muscle groups in the comparison between conditions 1 and 3. In particular, statistically significant differences were found between the two conditions in the right upper trapezius muscle group. [Conclusion] These findings suggest that the effect of stretching and stabilization exercises can reduce muscle fatigue in mobile phone users.