Abstract
Objective: To study the expression level of TRF1 (telomeric repeat binding factor 1) protein in human acute leukemia and relationship between expression level of TRF1 protein and telomerase. Methods: A quantitative Western-Blot technique was developed using anti-TRF133–277 monoclonal antibody and GST-TRF1 pruity protein as a standard to further determine the expression level of TRF1 protein in total proteins extracted from clinical specimens. Results: Bone marrow tissues of 20 acute leukemia patients were studied, 11 healthy donors’ bone marrows were taken as a control. The expression level of TRF1 protein was significantly higher (P<0.01) in normal bone marrow ((2.217±0.462) μg/μl) than that of acute leukemia patients ((0.754±0.343) μg/μl). But there was no remarkable difference between ALL and ANLL patients ((0.618±0.285) μg/μl vs (0.845±0.359) μg/μl, P>0.05). After chemotherapy, TRF1 expression level of patients with complete remission elevated ((0.772±0.307) μg/μl vs (1.683±0.344) μg/μl, P<0.01), but lower than that of normal ((2.217±0.462) μg/μl, P<0.01). There was no significantly difference after chemotherapy ((0.726±0.411) μg/μl vs (0.895±0.339) μg/μl, P>0.05). TRF1 expression level of patients with complete remission is higher than that of patients without complete remission ((1.683±0.344) μg/μl vs (0.895±0.339) μg/μl, P<0.01). All samples were determined for telomerase activity. It was confirmed that the activity of telomerase in normal bone marrow was lower than that of acute leukemia patients ((0.125±0.078) μg/μl vs (0.765±0.284) μg/μl, P<0.01). There was no significant difference of expression level of TRF1 protein between ALL and ANLL patients ((0.897±0.290) μg/μl vs (0.677±0.268) μg/μl, P>0.05). After chemotherapy, telomerase activity of patients with complete remission decreased ((0.393±0.125) μg/μl), but was still higher than that of normal ((0.125±0.078) μg/μl, P<0.01). Conclusion: The expression level of TRF1 protein has correlativity to the activity of telomerase (P<0.001).
Similar content being viewed by others
References
Aragona, M., Buda, C.A., Panetta, S., Morelli, M., Giudice, A., Campagna, F.L., Pontoriero, A., Cascinu, S., La Torre, F., 2000. Immunohistochemical telomeric-repeat binding factor-1 expression in gastrointestinal tumors. Oncol. Rep., 7(5):987–990.
Aragona, M., de Divitiis, O., La Torre, D., Panetta, S., D’Avella, D., Pontoriero, A., Morell, M., La Torre, I., Tomasello, F., 2001. Immunohistochemical TRF1 expression in human primary intracranial tumors. Anticancer Res., 21(3C):2135–2139.
Hiayma, E., Kodama, T., Shinbara, K., Iwao, T., Itoh, M., Hiyama, K., Shay, J.W., Matsuura, Y., Yokoyama, T., 1997. Telomease activity is detected in pancreatic cancer but not in benign tumors. Cancer Res., 57(2):326–331.
Huang, H., Chen, Q.F., Lin, M.F., 2000. Cloning and high level expression of telomeric repeat binging factor 1 33–277 in Escherichia coli. Journal of Zhejiang University (Medical Sciences), 29(5):193–194 (in Chinese).
Huang, H., Shi, J.M., Chen, Q.F., Luo, Y., Ding, W., Lou, J., 2002. Preparation and characterization of monoclonal antibody against human telomeric repeat binding factor 1. Chinese Journal of Hematology, 23(12):629–631 (in Chinese).
LaTorre, D., de Divitiis, O., Conti, A., Angileri, F.F., Cardali, S., Aguennouz, M., Aragona, M., Panetta, S., D’Avella, D., Vita, G., et al., 2005. Expression of telomeric repeat binding factor-1 in astroglial brain tumors. Neurosurgery, 56(4):802–810. [doi:10.1227/01.NEU.0000156468.41461.6F]
Luo, J., Bosy, T.Z., Wang, Y., Yasuda, R.P., Wolfe, B.B., 1996. Ontogeny of NMDA R1 subunit protein expression in five regions of rat brain. Developmental Brain Research, 92(1):10–17. [doi:10.1016/0165-3806(95)00191-3]
Ohyashiki, J.H., Hayashi, S., Yahata, N., Iwama, H., Ando, K., Tauchi, T., Ohyashiki, K., 2001. Impaired telomere regulation mechanism by TRF1 (telomere-binding protein), but not TRF2 expression, in acute leukemia cells. Int. J. Oncol., 18:593–598.
vanSteensel, B., de Large, T., 1997. Control of telomere length by the human telomeric protein TRF1. Nature, 385(6618):740–743. [doi:10.1038/385740a0]
Yamada, K., Yajima, T., Yagihashi, A., Kobayashi, D., Koyanagi, Y., Asanuma, K., Yamada, M., Moriai, R., Kameshima, H., Watanabe, N., 2000. Role of human telomerase reverse transcriptase and telomeric-repeat binding factor proteins 1 and 2 in human hematopoietic cells. Jpn. J. Cancer Res., 91(12):1278–1284.
Author information
Authors and Affiliations
Corresponding author
Additional information
Project (No. 2002CB713704) supported by the National Basic Research Program (973) of China
Rights and permissions
About this article
Cite this article
Shi, Jm., Huang, H., Chen, Qf. et al. A study of the relationship between expression level of TRF1 protein and telomerase activity in human acute leukemia. J. Zhejiang Univ. - Sci. B 7, 154–158 (2006). https://doi.org/10.1631/jzus.2006.B0154
Received:
Accepted:
Issue Date:
DOI: https://doi.org/10.1631/jzus.2006.B0154