ISHS Acta Horticulturae 859: International Symposium on Molecular Markers in Horticulture
GENETIC MARKERS FOR IDENTIFICATION OF A MALAYSIAN MEDICINAL MUSHROOM, LIGNOSUS RHINOCERUS (CENDAWAN SUSU RIMAU) |
| Authors: | C.S. Tan, S.T. Ng, S. Vikineswary, F.P. Lo, C.S. Tee |
| Keywords: | Lignosus rhinocerus, tiger¿s milk mushroom, internal transcribed spacer (ITS), 5.8S rDNA, phylogenetic analysis |
| DOI: | 10.17660/ActaHortic.2010.859.19 |
| Abstract:
Lignosus rhinocerus (“Tiger’s Milk” mushroom, or local Malay name “cendawan susu rimau (harimau)”) is one of the most important traditional medicinal mushrooms found in Malaysia. The local communities have been using this mushroom to treat asthma, fever, cough, cancer, food poisoning, wound healing, as a tonic, as well as to improve immunity and maintain general health. In this study, five isolates of L. rhinocerus from three different geographical regions in Malaysia with distances of more than 100 km were collected, and their internal transcribed spacer (ITS) regions of ribosomal RNA were sequenced and compared. Although these isolates were sampled from different geographical locations, they shared high nucleotide identity in ITS regions. A specific primer was designed based on the DNA sequences within the ITS1 region and paired with ITS4 primer in order to use as specific PCR-based genetic marker for identifying L. rhinocerus. | |
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