The first 3D-bioprinted personalized active bone to repair bone defects: A case report

Authors

  • Yongqiang Hao Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China
  • Bojun Cao Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China
  • Liang Deng Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China
  • Jiaxin Li Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical University, Harbin, China
  • Zhaoyang Ran Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China
  • Junxiang Wu Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China
  • Boran Pang Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China
  • Jia Tan Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China
  • Dinghao Luo Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China
  • Wen Wu Clinical and Translational Research Center for 3D Printing Technology, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Key Laboratory of Orthopaedic Implants, Department of Orthopaedic Surgery, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China；Shanghai Engineering Research Center of Innovative Orthopaedic Instruments and Personalized Medicine, Shanghai Ninth People’s Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai, 200011, China

DOI:

https://doi.org/10.18063/ijb.v9i2.654

Keywords:

Bone defect, 3D bioprinting, Personalized active bone, Platelet-rich plasma

Abstract

The repair and reconstruction of bone defects are still major problems to be solved in the field of orthopedics. Meanwhile, 3D-bioprinted active bone implants may provide a new and effective solution. In this case, we used bioink prepared from the patient’s autologous platelet-rich plasma (PRP) combined with polycaprolactone/β-tricalcium phosphate (PCL/β-TCP) composite scaffold material to print personalized PCL/β-TCP/ PRP active scaffolds layer by layer through 3D bioprinting technology. The scaffold was then applied in the patient to repair and reconstruct bone defect after tibial tumor resection. Compared with traditional bone implant materials, 3D-bioprinted personalized active bone will have significant clinical application prospects due to its advantages of biological activity, osteoinductivity, and personalized design.

References

Roddy E, DeBaun MR, Daoud-Gray A, et al., 2018, Treatment of critical-sized bone defects: Clinical and tissue engineering perspectives. Eur J Orthop Surg Traumatol, 28: 351–362. https://doi.org/10.1007/s00590-017-2063-0

Li J, Lai Y, Li M, et al., 2022, Repair of infected bone defect with clindamycin-tetrahedral DNA nanostructure complex-loaded 3D bioprinted hybrid scaffold. Chem Eng J, 435: 134855. https://doi.org/10.1016/j.cej.2022.134855

Agarwal R, Garcia AJ, 2015, Biomaterial strategies for engineering implants for enhanced osseointegration and bone repair. Adv Drug Deliv Rev, 94: 53–62. https://doi.org/10.1016/j.addr.2015.03.013

Kruyt MC, van Gaalen SM, Oner FC, et al., 2004, Bone tissue engineering and spinal fusion: The potential of hybrid constructs by combining osteoprogenitor cells and scaffolds. Biomaterials, 25: 1463–1473. https://doi.org/10.1016/s0142-9612(03)00490-3

Visscher DO, Farre-Guasch E, Helder MN, et al., 2016, Advances in bioprinting technologies for craniofacial reconstruction. Trends Biotechnol, 34: 700–710. https://doi.org/10.1016/j.tibtech.2016.04.001

Ashammakhi N, Hasan A, Kaarela O, et al., 2019, Advancing frontiers in bone bioprinting. Adv Healthc Mater, 8: 1801048. https://doi.org/10.1002/adhm.201801048

Menezes DJ, Shibli JA, Gehrke SA, et al., 2017, Effect of platelet-rich plasma in alveolar distraction osteogenesis: A controlled clinical trial. Br J Oral Maxillofac Surg, 55: 447–447

https://doi.org/10.1016/j.bjoms.2016.02.003

Li SH, Liu YH, Tian TR, et al., 2021, Bioswitchable delivery of microRNA by framework nucleic acids: Application to bone regeneration. Small, 17: 2104359. https://doi.org/10.1002/smll.202104359

Masaeli R, Zandsalimi K, Rasoulianboroujeni M, et al., 2019, Challenges in three-dimensional printing of bone substitutes. Tissue Eng Part B Rev, 25: 387–397. https://doi.org/10.1089/ten.teb.2018.0381

Matai I, Kaur G, Seyedsalehi A, et al., 2020, Progress in 3D bioprinting technology for tissue/organ regenerative engineering. Biomaterials, 226: 119536. https://doi.org/10.1016/j.biomaterials.2019.119536

Wuest S, Godla ME, Mueller R, et al., 2014, Tunable hydrogel composite with two-step processing in combination with innovative hardware upgrade for cell-based three-dimensional bioprinting. Acta Biomater, 10: 630–640. https://doi.org/10.1016/j.actbio.2013.10.016

Zheng X, Huang J, Lin J, et al., 2019, 3D bioprinting in orthopedics translational research. J Biomater Sci Polym Ed, 30: 1172–1187. https://doi.prg/10.1080/09205063.2019.1623989

Byambaa B, Annabi N, Yue K, et al., 2017, Bioprinted osteogenic and vasculogenic patterns for engineering 3D bone tissue. Adv Healthc Mater, 6: 1700015. 10.1002/ adhm.201700015

Shahabipour F, Ashammakhi N, Oskuee RK, et al., 2020, Key components of engineering vascularized 3-dimensional bioprinted bone constructs. Transl Res, 216: 57–76. https://doi.org/10.1016/j.trsl.2019.08.010

Santos MI, Reis RL, 2010, Vascularization in bone tissue engineering: Physiology, current strategies, major hurdles and future challenges. Macromol Biosci, 10: 12–27. https://doi.org/10.1002/mabi.200900107

Kamath MS, Ahmed SS, Dhanasekaran M, et al., 2014, Polycaprolactone scaffold engineered for sustained release of resveratrol: Therapeutic enhancement in bone tissue engineering. Int J Nanomed, 9: 183–195. https://doi.org/10.2147/ijn.s49460

Walsh WR, Vizesi F, Michael D, et al., 2008, Beta-TCP bone graft substitutes in a bilateral rabbit tibial defect model. Biomaterials, 29: 266–271. https://doi.org/10.1016/j.biomaterials.2007.09.035

Wei L, Wu S, Kuss M, et al., 2019, 3D printing of silk fibroin-based hybrid scaffold treated with platelet rich plasma for bone tissue engineering. Bioactive Mater, 4: 256–260. https://doi.org/10.1016/j.bioactmat.2019.09.001

Marx RE, Carlson ER, Eichstaedt RM, et al., 1998, Platelet-rich plasma-Growth factor enhancement for bone grafts. Oral Surg Oral Med Oral Pathol Oral Radiol Endod, 85: 638–646. https://doi.org/10.1016/s1079-2104(98)90029-4

Downloads

Published

2022-12-22

Issue

Vol. 9 No. 2 (2023): International Journal of Bioprinting

Section

Clinical Case Study

License

Copyright (c) 2024 International Journal of Bioprinting

Creative Commons License

This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License.