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Galactose-functionalized Magnetic Iron-oxide Nanoparticles for Enrichment and Detection of Ricin Toxin

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Abstract

To generate a new specific recognition module for the enrichment and detection of ricin, galactose-functionalized silanized magnetic iron-oxide nanoparticles (Gal-SiMNPs) were assembled by amino-silanized MNPs (amino-SiMNPs) and a galactose linker. Amino-SiMNPs were produced by a coprecipitation method, and were coated with double layers of silica and aminosilane by hydrolysis of tetraethoxysilane and 3-aminopropyltriethoxysilane, respectively. A galactose with an amido-acid linker was synthesized by four steps of chemical modification from O-acetyl protected galactose. The diameters of Gal-SiMNPs were characterized as being 60 ± 20 nm. The average amount of galactose-loading and ricin-binding on Gal-SiMNPs was 30 ± 2 μg galactose and 29 ± 2 μg ricin toxin on the surface of 1 mg of Gal-SiMNPs, respectively. Furthermore, a rapid, simple and efficient colorimetric assay was established for the detection of ricin based on the Gal-SiMNPs, and the limits of detection (LODs) of 2 and 4 ng/mL for ricin in physiological buffer and serum were obtained, respectively.

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Authors and Affiliations

  1. Beijing Institute of Pharmacology and Toxicology, No.27 Tai Ping Road, Hai Dian District, Beijing, 100850, P. R. China

    He-Zhu Liu, Ji-Jun Tang, Xiao-Xi Ma, Lei Guo, Jian-Wei Xie & Yu-Xia Wang

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  1. He-Zhu Liu
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  2. Ji-Jun Tang
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  3. Xiao-Xi Ma
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  4. Lei Guo
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  6. Yu-Xia Wang
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Correspondence to Jian-Wei Xie.

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Liu, HZ., Tang, JJ., Ma, XX. et al. Galactose-functionalized Magnetic Iron-oxide Nanoparticles for Enrichment and Detection of Ricin Toxin. ANAL. SCI. 27, 19–24 (2011). https://doi.org/10.2116/analsci.27.19

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  • DOI: https://doi.org/10.2116/analsci.27.19

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