Exercise Training Enhances Tumor Necrosis Factor-α–Induced Expressions of Anti-Apoptotic Genes without Alterations in Caspase-3 Activity in Rat Epididymal Adipocytes

Takuya Sakurai; Megumi Takei; Junetsu Ogasawara; Naoto Watanabe; Minori Sampei; Midori Yoshida; Dai Nakae; Tomonobu Sakurai; Norihiko Nakano; Takako Kizaki; Hideki Ohno; Tetsuya Izawa

doi:10.2170/jjphysiol.R2096

Regular Papers

Exercise Training Enhances Tumor Necrosis Factor-α–Induced Expressions of Anti-Apoptotic Genes without Alterations in Caspase-3 Activity in Rat Epididymal Adipocytes

Takuya Sakurai, Megumi Takei, Junetsu Ogasawara, Naoto Watanabe, Minori Sampei, Midori Yoshida, Dai Nakae, Tomonobu Sakurai, Norihiko Nakano, Takako Kizaki, Hideki Ohno, Tetsuya Izawa

Author information

Takuya Sakurai
Department of Kinesiology, Graduate School of Science, Tokyo Metropolitan University
Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine
Megumi Takei
Department of Kinesiology, Graduate School of Science, Tokyo Metropolitan University
Junetsu Ogasawara
Department of Kinesiology, Graduate School of Science, Tokyo Metropolitan University
Naoto Watanabe
Department of Pathology, Sasaki Institute
Minori Sampei
Department of Kinesiology, Graduate School of Science, Tokyo Metropolitan University
Midori Yoshida
Department of Pathology, Sasaki Institute
Dai Nakae
Department of Pathology, Sasaki Institute
Tomonobu Sakurai
Department of Kinesiology, Graduate School of Science, Tokyo Metropolitan University
Norihiko Nakano
Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine
Takako Kizaki
Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine
Hideki Ohno
Department of Molecular Predictive Medicine and Sport Science, Kyorin University, School of Medicine
Tetsuya Izawa
Department of Kinesiology, Graduate School of Science, Tokyo Metropolitan University

Keywords: exercise training, TNF-α, anti-apoptotic gene, lipogenic gene, rat adipocyte

JOURNAL FREE ACCESS

2005 Volume 55 Issue 3 Pages 181-189

DOI https://doi.org/10.2170/jjphysiol.R2096

View "Advance Publication" version (August 31, 2005).

Details

Abstract

The effect of exercise training on tumor necrosis factor-α (TNF-α) signaling was investigated in rat epididymal adipocytes. Incubation of isolated adipocytes with TNF-α (20 ng/ml) for 5 h enhanced the expression of the inhibitor apoptosis protein 2 (IAP2) gene without any enhancement of caspase-3 activity in both the sedentary control (C) and exercise-trained (TR) groups. However, the ability of TNF-α to enhance IAP2 gene expression was significantly greater in TR than in C rats. The basal expression of the IÎºB kinase β (IKK β) gene and that of the BCL-x_L gene were also higher in TR than in C rats. Mn-superoxidedismutase contents in adipocytes were higher in TR than in C rats. Moreover, no apoptotic nucleuses of adipocytes in response to acute exercise were observed in either group at least up to 5 h after exercise. Exercise training also enhanced the inhibitory effect of TNF-α on the gene expression of the fatty acid synthase (FAS), a lipogenic enzyme, suggesting that fatty acid synthesis may be reduced. Thus, exercise training enhanced TNF-α signaling directed toward the expressions of survival signals and the suppression of FAS gene expression.

Corresponding author

Register with J-STAGE for free!