doiSerbia

Ameliorative effects of the ethanolic extract of Allium saralicum R.M. Fritsch on CCl4-induced nephrotoxicity in mice: A stereological examination

Sherkatolabbasieh Hamidreza (Lorestan University of Medical Sciences, School of Medicine, Department of Pediatrics, Khoramabad, Iran)
Hagh-Nazari Lida (Kermanshah University of Medical Science, Faculty of Medicine, Department of Biochemistry, Kermanshah, Iran)
Shafiezadeh Shiva (Lorestan University of Medical Sciences, Department of Internal Medicine, Khoramabad, Iran)
Goodarzi Nader (Razi University, Faculty of Veterinary Medicine, Department of Basic Science, Kermanshah, Iran)
Zangeneh Mohammad Mehdi (Razi University, Faculty of Veterinary Medicine, Kermanshah, Iran)
Zangeneh Akram (Razi University, Faculty of Veterinary Medicine, Kermanshah, Iran)

The present study was carried out to investigate the nephroprotective effect of the ethanolic extract of Allium saralicum R.M. Fritsch (ASRMF) in mice. Thirty-five male mice were divided into five groups (n=7). Group 1 (positive control) received 1 mL/kg olive oil intraperitoneally (i.p.) and 0.5 mL distilled water orally; Group 2 (negative control) received CCl4 (50% in olive oil, 1 mg/kg; i.p.); Groups 3, 4 and 5 received CCl4 and 200, 800 and 1600 μg/kg of ASRMF extract, respectively. The renal volume and cortex in Groups 1 and 2 were increased by 55% and 62% (p≤0.001) following CCl4 administration, respectively, and were improved after ASRMF administration. The volume of proximal convoluted tubules (PCTs), glomeruli, vessels and interstitial tissue increased 80%, 150%, 83% and 64% (p≤0.05), respectively, in CCl4-treated mice, and decreased significantly with 800 and 1600 μg/kg of ASRMF. The length of PCTs and vessels increased 51% and 45% and decreased (p≤0.05) with 200, 800 and 1600 μg/kg of ASRMF, respectively. CCl4-treated mice lost 22.5% of glomeruli; the loss was inhibited significantly (p≤0.05) by ASRMF. Urea and creatinine concentrations were increased (p≤0.05) in CCl4-induced nephrotoxicity as compared to the controls, whereas different doses of ASRMF restored the levels of these biomarkers compared to the negative controls. In conclusion, ASRMF has a potent nephroprotective property and can improve renal structural and serum biomarkers in CCl4-induced nephrotoxicity in mice.

Keywords: carbon tetrachloride, Allium saralicum, kidney, nephrotoxicity, stereology