doiSerbia

Edible mycorrhizal species Lactarius controversus Pers. 1800 as a source of antioxidant and cytotoxic agents

Novaković Aleksandra R. (Institute of Food Technology, Novi Sad)
Karaman Maja A. (Faculty of Sciences, Department of Biology and Ecology, Novi Sad)
Milovanović Ivan Lj. (Institute of Food Technology, Novi Sad)
Belović Miona M. (Institute of Food Technology, Novi Sad)
Rašeta Milena J. (Faculty of Sciences, Department of Chemistry, Biochemistry and Environmental Protection, Novi Sad)
Radusin Tanja I. (Institute of Food Technology, Novi Sad)
Ilić Nebojša M. (Institute of Food Technology, Novi Sad)

The aim of this work was to study chemical profile and antioxidant and cytotoxic activities of ethanol and water extracts of Lactarius controversus (Pers.) 1800 mushroom species growing in eastern Serbia. The chemical characterization of phenolic compounds performed by HPLC-MS/MS demonstrated the presence of quinic acid among others. Determination of antioxidant activity, including radical scavenging effects on DPPH•, NO•, OH• and SOA radicals and ferric reducing ability was investigated. The highest DPPH radical scavenging effect was obtained for water extract (LcAq) while ethanol extract (LcEtOH) demonstrated the highest FRAP activity. Hexane extract applied in antibacterial assay against three pathogenic strains demonstrated antibacterial effect only against S. aureus ATCC25922. Anti-proliferative properties against estrogen dependent MCF 7 breast cancer cell lines using MTT showed higher activity for ethanolic extract.

Keywords: Lactarius controversus, phenolic compounds, quinic acid, antioxidants, cytotoxic agents

Projekat Ministarstva nauke Republike Srbije, br. III 46001 i br. 172058