doiSerbia

Hydrogen-rich PBS protects cultured human cells from ionizing radiation-induced cellular damage

Qian Liren (Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, P. R. China)
Li Bailong (Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, P. R. China)
Cao Fei (Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, P. R. China)
Huang Yuecheng (Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, P. R. China)
Liu Shulin (Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, P. R. China)
Cai Jianming (Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, P. R. China)
Gao Fu (Department of Radiation Medicine, Faculty of Naval Medicine, Second Military Medical University, Shanghai, P. R. China)

Hydroxyl radicals play an important role in ionizing radiation-induced cellular damage, while hydrogen can selectively reduce hydroxyl radicals in vitro. This study was designed to test the hypothesis that hydrogen-rich PBS may be an effective radioprotective agent in vitro. Compared to cells pretreated without hydrogen, we demonstrated that treating cells with hydrogen-rich PBS before irradiation could significantly inhibit IR-induced apoptosis, increase viability of human intestinal crypt cells, significantly increase endogenous antioxidant, and decrease malondialdehyde and 8-hydroxydeoxyguanosine concentrations of human lymphocyte AHH-1 cells. It is concluded that hydrogen has a potential as an effective and safe radioprotective agent.

Keywords: hydrogen, ionizing radiation, radioprotection, apoptosis, reactive oxygen species