doiSerbia

Analysis of polymorphism in the survivin gene promoter as a potential risk factor for head and neck cancers development

Kostić Marija (Stomatološki fakultet, Institut za humanu genetiku, Beograd)
Nikolić Nađa (Stomatološki fakultet, Institut za humanu genetiku, Beograd)
Ilić Branislav (Stomatološki fakultet, Institut za humanu genetiku, Beograd)
Čarkić Jelena (Stomatološki fakultet, Institut za humanu genetiku, Beograd)
Milenković Sanja (Kliničko-bolnički centar Zemun, Služba kliničke patologije, Beograd)
Vukadinović Miroslav (Stomatološki fakultet, Klinika za maksilofacijalnu hirurgiju, Beograd)

Introduction. Association studies have shown that gene polymorphisms in various classes of genes can modulate cancer risk. The -31G/C polymorphism in the promoter of survivin gene, affects the expression of the anti-apoptotic protein survivin which in turn may predispose an individual to some types of cancer. Objective. The aim of the study was to determine whether the survivin promoter -31G/C polymorphism could be a susceptibility factor for squamous cell carcinoma (SCC) of the oral cavity and basal cell carcinoma (BCC) of the skin. Methods. The DNA obtained from 88 patients with SCC, 60 patients with BCC and 111 healthy individuals was subjected to polymerase chain reaction-restriction fragment length polymorphism analysis (PCR- RFLP) in order to determine genotype and allele frequencies in patients and control groups. Logistic regression was used for cancer risk assessment. Results. The following distribution of genotypes was obtained: CC genotype 15% in the SCC group, 13% in the BCC group and 12% in controls; CG genotype 41% in SCCs, 35% in BCCs, 48% in controls; GG genotype 44% in SCCs, 52% in BCCs and 40% in controls. Allelic frequencies were as follows: G allele 0.65 in SCCs, 0.69 in BCCs and 0.64 in the control group; C allele 0.35 in SCCs, 0.31 in BCCs and 0.36 in the control group. There was no statistically significant difference in allele or genotype frequencies between the patients and controls (p>0.05). Conclusion. In Serbian population, -31G/C polymorphism in the promoter of the survivin gene cannot be considered as a risk factor for oral squamous cell carcinoma and skin basal cell carcinoma.

Keywords: polymorphism, survivin, promoter, head and neck cancers

Projekat Ministarstva nauke Republike Srbije, br. 175075