Vojnosanitetski pregled 2016 Volume 73, Issue 1, Pages: 16-20
https://doi.org/10.2298/VSP140428124L
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The expression and significance of p53 protein and Ki-67 protein in pterygium
Ljubojević Vesna (University of Banja Luka, Faculty of Medicine, Banja Luka, Bosnia and Herzegovina + Clinical Center Banja Luka, Department of Histology and Embriology, Banja Luka, Bosnia and Herzegovina)
Gajanin Radoslav (University of Banja Luka, Faculty of Medicine, Banja Luka, Bosnia and Herzegovina + Clinical Center Banja Luka, Department of Pathology, Banja Luka, Bosnia and Herzegovina)
Amidžić Ljiljana (University of Banja Luka, Faculty of Medicine, Banja Luka, Bosnia and Herzegovina + Clinical Center Banja Luka, Department of Histology and Embriology, Banja Luka, Bosnia and Herzegovina)
Vujković Zoran (University of Banja Luka, Faculty of Medicine, Banja Luka, Bosnia and Herzegovina + Clinical Center Banja Luka, Clinic of Neurology, Banja Luka, Bosnia and Herzegovina)
Background/Aim. Pterygium is considered to be a degenerative disease of the
conjunctiva, however, the presence of tumor markers in pterygium reinforces
the hypothesis that this lesion is similar to tumor. Inactivation of p53
function removes an obstacle to increased proliferation. Factors affecting
the prevalence of p53 expression in pterygium deserve investigation. The
aim of the study was to investigate the expression of p53 and Ki-67 proteins
in pterygium and normal conjunctiva, the effects of gender and age on p53
expression, and the relationship between the expression of p53 and Ki-67
proteins. Methods. A total of 34 samples of pterygium and 34 samples of the
normal conjunctiva were analyzed. The samples were studied by
immunohistochemistry using antibodies against p53 and Ki-67. Results.
Totally 15 (44%) samples of pterygia were p53 positive. Correlations
between the expression of p53 protein and sex, and age were not established.
The number of Ki-67 positive cells in pterygium (9.74%) was significantly
higher than the number of Ki-67 positive cells in the normal conjunctiva
(1.74%), (p = 0.001). Between the expression of p53 protein and Ki-67
protein in pterygium there was a significant positive correlation (p =
0.000). Conclusion. The prevalence of p53 positive samples of pterygium was
44%. The influence of sex and age on p53 protein expression in pterygium was
not found. The increased proliferative acivity was present in the epithelium
of pterygium. The expression of Ki-67 protein is associated with the
expression of p53 protein in pterygium. The findings of our study support
the thesis of pterygium as tissue growth disorder.
Keywords: pterygium, tumor markers, biological, tumor suppressor protein p53, conjunctiva, age factos, sex, immunohistochemistry