Archives of Biological Sciences 2015 Volume 67, Issue 2, Pages: 535-545
https://doi.org/10.2298/ABS141009016P
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Effect of aspartame on biochemical and oxidative stress parameters in rat blood
Prokić Marko D. (Institute for Biological Research "Siniša Stanković", Department of Physiology, Belgrade)
Paunović Milica G. (Faculty of Science, Institute of Biology and Ecology, Kragujevac)
Matić Miloš M. (Faculty of Science, Institute of Biology and Ecology, Kragujevac)
Đorđević Nataša Z. (State University of Novi Pazar, Department of Biomedical Sciences, Novi Pazar)
Ognjanović Branka I. (Faculty of Science, Institute of Biology and Ecology, Kragujevac)
Štajn Andraš Š. (Faculty of Science, Institute of Biology and Ecology, Kragujevac)
Saičić Zorica S. (Institute for Biological Research "Siniša Stanković", Department of Physiology, Belgrade)
Aspartame (ASP) is one of the most widely used nonnutritive sweeteners. This
study investigates the chronic effects of ASP on hematological and
biochemical parameters, and its effects on the oxidative/antioxidative status
in the red blood cells of Wistar albino rats. Rats were provided with ASP (40
mg/kg/daily for six weeks) in drinking water. Increased food and fluid intake
was observed in the ASP-treated rats. Total body mass was significantly
decreased in the ASP-treated rats. Treatment with ASP caused an increase in
the concentrations of glucose, cholesterol, LDL-cholesterol, and in the
activities of alanine aminotransferase (ALT), aspartate aminotransferase
(AST) and lactate dehydrogenase (LDH), as well as a decrease in the levels of
HDL-cholesterol in the serum. A significant decline in the number of white
blood cells (WBC) was observed after ASP uptake. Based on the results we
conclude that ASP induces oxidative stress, observed as an alteration of the
glutathione redox status, which leads to increased concentrations of nitric
oxide (NO) and lipid peroxides (LPO) in the red blood cells. Changes in
biochemical parameters, lipid metabolism, as well as changes in the levels of
oxidative stress markers and the appearance of signs of liver damage indicate
that chronic use of ASP can lead to the development of hyperglycemia,
hypercholesterolemia and associated diseases.
Keywords: Aspartame, hematological parameters, biochemical parameters, oxidative stress, appetite
Projekat Ministarstva nauke
Republike Srbije, br. 173041