This cone beam computed tomography (CBCT) study aimed to describe the maxillary premolar anatomy of a South African subpopulation using two classification systems. A total of 601 premolars were evaluated. For each tooth, the root number and canal configurations were described using the classification systems devised by Vertucci (1984) and Ahmed et al. (2017). Correlations between root number and sex were determined using the chi-squared test (P = 0.05). Two roots were present in approximately half of all maxillary first premolars (54.1%, n = 171/316). The majority of maxillary second premolars displayed one root (78.2%, n = 223/285). Single-rooted maxillary second premolars were more common in females (P < 0.05). The Vertucci type IV configuration was most prevalent in the maxillary first premolars. In contrast, maxillary second premolars showed a greater tendency toward Vertucci’s type I configuration. The classification proposed by Ahmed et al. indicated the most prevalent maxillary first premolar configuration to be ²MP B¹P¹. The most common configuration among the maxillary second premolars was ¹ MP¹. Diverse root and canal anatomical presentations were found in this subpopulation. Both classification systems adequately describe maxillary premolar anatomy; however, the system proposed by Ahmed et al. may more accurately describe complex teeth.