Evaluation of antioxidant activity of flower and seed oil of Azadirachta indica A. juss
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Abstract
The present study on evaluation of antioxidant activity of the flowers and seed oil of Neem plant Azardirachta indica A. Juss revealed that the ethanolic extract of flowers and seed oil at 200 ?g/ml producing the highest free radical scavenging activity i.e. 64.17±0.02% and 66.34±0.06% respectively. The Neem oil has the highest amount of total phenol content (132 ?g/ml) which is responsible for highest percentage of inhibition of DPPH radical. In conclusion Neem flower and seed oil have potential for use in human health which is used as food by common people and in diabetes and Neem seed oil is widely used for variety of diseases and also antioxidant potential for use in different pharmaceutical industries.
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Keywords
Neem oil, Flower, Antioxidant activity, DPPH scavenging assay
References
Aromdee, C. and Sriubolmas, N. (2006). Essential oil of the flowers of Azardirachta indica A. Juss (Meliaceae). Journal of Science and Technology, 28(1): 115-119.
Baratta, M.T., Dorman, H.J.D., Deans, S.G., Figueiredo, C., Barroso, J.G. and Ruberto, G. (1998). Antimicrobial and antioxidant properties of some commercial essential oils. Flav. Fragr. J., 13: 235 - 244.
Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaft and Technologie, 28:25-30.
Colgate, S.M., Molyneux, R.J. (1993). Bioactive natural products - detection, isolation, and structural determination. CRC Press 9-34.
Deans, S.G., Noble, R.C., Penzes, L. and Imre, S.G. (1993). Promotional effects of plant volatile oils on the polyunsaturated fatty acid status during aging. Age, 16 : 71-74.
Dorman, H.J.D., Deans, S.G., Noble, R.C., Surai, P. (1995). Evaluation in vitro of plant essential oils as natural antioxidants. J. Essent. Oil Res., 7: 645 - 651.
Ghimeray, A.K., Jin,C.W., Ghimire,B.K., Cho, D.H. (2009). Antioxidant activity and quantitative estimation of azadirachtin and nimbin in Azardirachta indica A. Juss grown in foothills of Nepal. African Journal of Bio-technology, 8 (33):3084-3091.
Gonçalves, J.L.S., Lopes, R.C., Oliveira, D.B., Costa, S.S., Miranda, M.M.F.S., Romano, M.T.V., Santos, N.S.O., Wigg, M.D. (2005). In vitro anti-rotavirus activity of some medicinal plants used in Brazil against diarrhea. Journal of Ethnopharmacology, 99 (3):403–407.
Gupta, H. (2008). Neem: A Natural Boon. Pharmainfo.net. 6(5):1-11.
Katsube, T., Tabata, H., Ohta, Y., Yamasaki, Y., Anuurad, E., Shiwaku, K., Yamane, Y. (2004). Screening for antioxidant activity in edible plant products: Comparison of low density lipoprotein oxidation assay. Journal of Agriculture and Food Chemistry, 52:2391-2396.
Kim, K.T., Yoo, K.M., Lee, J.W., Eom, S.H., Hwang, I.K., Lee, C.Y. (2007). Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress. J. Ethnopharm., 111: 443-445.
Manuel Viuda-Martos, Yolanda Ruiz Navajas, Elena Sánchez Zapata, Juana Fernández-López and José A. Pérez-Álvarez. (2010). Antioxidant activity of essential oils of five spice plants widely used in a Mediterranean diet. Flavour Fragr. J., 25:13-19.
Maria Yuliana Liauw, F. A. Natan, P. Widiyanti, D. Ikasari, N. Indraswati and F. E. Soetaredjo. (2008). Extraction of neem oil (Azardirachta indica A. Juss) using n-hexane and ethanol: studies of oil quality, kinetic and thermodynamic. ARPN Journal of Engineering and Applied Sciences, 3(3):49-54.
Olabinri, B. M., Adebisi, J. A., Odesomi, O. F., Olabinri, P.F. and Adeleke, G. E. (2009). Experimental classification of the antioxidant capacity of the leaf, stem and root barks of Mengifera indica and A. indica. African Journal of Biotechnology, 8 (13): 2968-2972.
Oyedeji, O.A., Adayan, A.J. (2005). Chemical composition and antibacterial activity of essential oil of Centella asiatica growing in South Africa. Pharmaceutical Biology, 43 (3): 249-252.
Rashid, A., I. Ahmad, Iram S., Mirza J.I., Rauf, C.A. (2004). Efficiency of different Neem (Azardirachta indica A. Juss) products against various life stages of Phytophthora infestans (mont.) de Bary. Pak. J. Bot., 36(4): 881-886.
Svoboda, K.P., Deans, S.G. (1992). A study of the variability of rosemary and sage and their volatile oils on the British market: their antioxidative properties. Flav. Fragr. J., 7:81 - 87.
Svoboda, K., Hampson, J. and Hunter, E.A. (1998a). Production and bioactivity of essential oils in secretory tissues of higher plants. Proceedings of The World of Aromatherapy II Conference of the National Association for Holistic Aromatherapy (NAHA), 25 – 28 September, St. Louis, Missouri, USA, 105 – 127.
Svoboda, K.P., Inglis, A., Hampson, J., Galambosi, B., Asakawa Y. (1998b). Biomass production, essential oil yield and composition of Myrica gale L. harvested from wild populations in Scotland and Finland. Flav. Fragr. J., 13, 367-372.
Wadher, K.J., Lakhotia, C.L. and Umekar, M. J. (2009). Formulation and evolution of cream of Azardirachta indica A. Juss leave extracts on skin renewal rate. Int. J. of Chem. Tech. Research, 1:95.
Yang, J. H., Lin, H.C. and Mau, J. L. (2002). Antioxidant properties of several commercial mushrooms. Food Chem., 77: 229-235.
Baratta, M.T., Dorman, H.J.D., Deans, S.G., Figueiredo, C., Barroso, J.G. and Ruberto, G. (1998). Antimicrobial and antioxidant properties of some commercial essential oils. Flav. Fragr. J., 13: 235 - 244.
Brand-Williams, W., Cuvelier, M.E. and Berset, C. (1995). Use of free radical method to evaluate antioxidant activity. Lebensmittel Wissenschaft and Technologie, 28:25-30.
Colgate, S.M., Molyneux, R.J. (1993). Bioactive natural products - detection, isolation, and structural determination. CRC Press 9-34.
Deans, S.G., Noble, R.C., Penzes, L. and Imre, S.G. (1993). Promotional effects of plant volatile oils on the polyunsaturated fatty acid status during aging. Age, 16 : 71-74.
Dorman, H.J.D., Deans, S.G., Noble, R.C., Surai, P. (1995). Evaluation in vitro of plant essential oils as natural antioxidants. J. Essent. Oil Res., 7: 645 - 651.
Ghimeray, A.K., Jin,C.W., Ghimire,B.K., Cho, D.H. (2009). Antioxidant activity and quantitative estimation of azadirachtin and nimbin in Azardirachta indica A. Juss grown in foothills of Nepal. African Journal of Bio-technology, 8 (33):3084-3091.
Gonçalves, J.L.S., Lopes, R.C., Oliveira, D.B., Costa, S.S., Miranda, M.M.F.S., Romano, M.T.V., Santos, N.S.O., Wigg, M.D. (2005). In vitro anti-rotavirus activity of some medicinal plants used in Brazil against diarrhea. Journal of Ethnopharmacology, 99 (3):403–407.
Gupta, H. (2008). Neem: A Natural Boon. Pharmainfo.net. 6(5):1-11.
Katsube, T., Tabata, H., Ohta, Y., Yamasaki, Y., Anuurad, E., Shiwaku, K., Yamane, Y. (2004). Screening for antioxidant activity in edible plant products: Comparison of low density lipoprotein oxidation assay. Journal of Agriculture and Food Chemistry, 52:2391-2396.
Kim, K.T., Yoo, K.M., Lee, J.W., Eom, S.H., Hwang, I.K., Lee, C.Y. (2007). Protective effect of steamed American ginseng (Panax quinquefolius L.) on V79-4 cells induced by oxidative stress. J. Ethnopharm., 111: 443-445.
Manuel Viuda-Martos, Yolanda Ruiz Navajas, Elena Sánchez Zapata, Juana Fernández-López and José A. Pérez-Álvarez. (2010). Antioxidant activity of essential oils of five spice plants widely used in a Mediterranean diet. Flavour Fragr. J., 25:13-19.
Maria Yuliana Liauw, F. A. Natan, P. Widiyanti, D. Ikasari, N. Indraswati and F. E. Soetaredjo. (2008). Extraction of neem oil (Azardirachta indica A. Juss) using n-hexane and ethanol: studies of oil quality, kinetic and thermodynamic. ARPN Journal of Engineering and Applied Sciences, 3(3):49-54.
Olabinri, B. M., Adebisi, J. A., Odesomi, O. F., Olabinri, P.F. and Adeleke, G. E. (2009). Experimental classification of the antioxidant capacity of the leaf, stem and root barks of Mengifera indica and A. indica. African Journal of Biotechnology, 8 (13): 2968-2972.
Oyedeji, O.A., Adayan, A.J. (2005). Chemical composition and antibacterial activity of essential oil of Centella asiatica growing in South Africa. Pharmaceutical Biology, 43 (3): 249-252.
Rashid, A., I. Ahmad, Iram S., Mirza J.I., Rauf, C.A. (2004). Efficiency of different Neem (Azardirachta indica A. Juss) products against various life stages of Phytophthora infestans (mont.) de Bary. Pak. J. Bot., 36(4): 881-886.
Svoboda, K.P., Deans, S.G. (1992). A study of the variability of rosemary and sage and their volatile oils on the British market: their antioxidative properties. Flav. Fragr. J., 7:81 - 87.
Svoboda, K., Hampson, J. and Hunter, E.A. (1998a). Production and bioactivity of essential oils in secretory tissues of higher plants. Proceedings of The World of Aromatherapy II Conference of the National Association for Holistic Aromatherapy (NAHA), 25 – 28 September, St. Louis, Missouri, USA, 105 – 127.
Svoboda, K.P., Inglis, A., Hampson, J., Galambosi, B., Asakawa Y. (1998b). Biomass production, essential oil yield and composition of Myrica gale L. harvested from wild populations in Scotland and Finland. Flav. Fragr. J., 13, 367-372.
Wadher, K.J., Lakhotia, C.L. and Umekar, M. J. (2009). Formulation and evolution of cream of Azardirachta indica A. Juss leave extracts on skin renewal rate. Int. J. of Chem. Tech. Research, 1:95.
Yang, J. H., Lin, H.C. and Mau, J. L. (2002). Antioxidant properties of several commercial mushrooms. Food Chem., 77: 229-235.
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How to Cite
Evaluation of antioxidant activity of flower and seed oil of Azadirachta indica A. juss. (2011). Journal of Applied and Natural Science, 3(1), 78-81. https://doi.org/10.31018/jans.v3i1.158
